Erratum: Composite hemangioendothelioma with neuroendocrine marker expression: an aggressive variant

Perry, Kyle; Al-Lbraheemi, Alyaa; Rubin, Brian P.; Jen, Jin; Ren, Hongzheng; Jang, Jin Sung; Nair, Asha; Davila, Jaime; Pambuccian, Stefan E.; Horvai, Andrew E.; Sukov, William R.; Tazelaar, Henry D.; Folpe, Andrew L.

doi:10.1038/modpathol.2017.116

Cited by 10 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RNA exome capture detects more fusion genes and alternatively spliced genes compared to standard mRNA-Seq and total RNA-Seq in FFPE samples [ 8 , 9 , 12 ]. Also, previous reports showed that gene expression quantification data is comparable with mRNA-Seq in high-quality RNA samples and total RNA-Seq in degraded samples [ 11 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

“…High-quality RNA from fresh biological tissues is optimal to generate reliable transcriptome data. As FFPE samples are highly modified and fragmented with wide ranges of nucleotides, standard mRNA-Seq (poly-A selection) methods for transcriptome analysis are challenging [ 6 , 7 ]; total RNA-Seq (with rRNA depletion) or RNA exome capture are the preferred methods [ 8 – 10 ]. However, total RNA-Seq using FFPE RNA is not generally consistent likely due to variation in RNA quality, with an abundance of intronic, intergenic, and rRNA reads and fewer exonic reads [ 7 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Application of the 3′ mRNA-Seq using unique molecular identifiers in highly degraded RNA derived from formalin-fixed, paraffin-embedded tissue

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background Archival formalin-fixed, paraffin-embedded (FFPE) tissue samples with clinical and histological data are a singularly valuable resource for developing new molecular biomarkers. However, transcriptome analysis remains challenging with standard mRNA-seq methods as FFPE derived-RNA samples are often highly modified and fragmented. The recently developed 3′ mRNA-seq method sequences the 3′ region of mRNA using unique molecular identifiers (UMI), thus generating gene expression data with minimal PCR bias. In this study, we evaluated the performance of 3′ mRNA-Seq using Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit FWD with UMI, comparing with TruSeq Stranded mRNA-Seq and RNA Exome Capture kit. The fresh-frozen (FF) and FFPE tissues yielded nucleotide sizes range from 13 to > 70% of DV200 values; input amounts ranged from 1 ng to 100 ng for validation. Results The total mapped reads of QuantSeq 3′ mRNA-Seq to the reference genome ranged from 99 to 74% across all samples. After PCR bias correction, 3 to 56% of total sequenced reads were retained. QuantSeq 3′ mRNA-Seq data showed highly reproducible data across replicates in Universal Human Reference RNA (UHR, R > 0.94) at input amounts from 1 ng to 100 ng, and FF and FFPE paired samples (R = 0.92) at 10 ng. Severely degraded FFPE RNA with ≤30% of DV200 value showed good concordance (R > 0.87) with 100 ng input. A moderate correlation was observed when directly comparing QuantSeq 3′ mRNA-Seq data with TruSeq Stranded mRNA-Seq (R = 0.78) and RNA Exome Capture data (R > 0.67). Conclusion In this study, QuantSeq 3′ mRNA-Seq with PCR bias correction using UMI is shown to be a suitable method for gene quantification in both FF and FFPE RNAs. 3′ mRNA-Seq with UMI may be applied to severely degraded RNA from FFPE tissues generating high-quality sequencing data.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Application of the 3′ mRNA-Seq using unique molecular identifiers in highly degraded RNA derived from formalin-fixed, paraffin-embedded tissue

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…8,9 Despite this, while local recurrence is common, typical composite hemangioendotheliomas are less aggressive than angiosarcomas with only 1 reported case of metastasis and death. 10 Given its nebulous definition and variety of presentations, there is question as to whether hemangioendotheliomas may represent a spectrum of disease from hemangioma to angiosarcoma, more benign or aggressive variants of angiosarcomas themselves, or entirely separate entities altogether. 8,10…”

Section: Discussionmentioning

confidence: 99%

“…10 Given its nebulous definition and variety of presentations, there is question as to whether hemangioendotheliomas may represent a spectrum of disease from hemangioma to angiosarcoma, more benign or aggressive variants of angiosarcomas themselves, or entirely separate entities altogether. 8,10…”

Section: Discussionmentioning

confidence: 99%

Malignant Conversion of Eyelid Capillary Hemangioma to Cutaneous Angiosarcoma

Heinze

Pham

Lin

et al. 2021

Ophthalmic Plastic &Amp; Reconstructive Surgery

View full text Add to dashboard Cite

Angiosarcomas represent a rare aggressive malignancy of endothelial cell origin that carry a poor prognosis. Here, the authors report the case of a 71-year-old male presenting with a well-differentiated angiosarcoma of the right upper eyelid which arose at the site of a congenital eyelid hemangioma. The patient had undergone multiple surgical resections of the hemangioma previously without adjunctive radiation therapy. This lends evidence to the potential for malignant transformation of benign hemangiomas, and long-term monitoring of benign cutaneous vascular lesions should be considered.

show abstract

“…Herein, we report two cases of CHE, the first case occurred in the left pubis (female, 21 years old), and the second case is a patient post-resection of CHE in the manubrium sterni 10 years ago [ 1 ], who developed of new lesions in the left ilium and T6, T12 vertebra (male, 66 years old). To the best of our knowledge, it is the 3rd report describing CHE occurring originally in bone [ 1 , 6 ]. These cases highlighted the relative frequency of occurrence in bone.…”

Section: Introductionmentioning

confidence: 99%

Composite hemangioendothelioma- report of two cases located in bone and review of the literature

Deng,

2023

BMC Musculoskelet Disord

View full text Add to dashboard Cite

Background Composite hemangioendothelioma (CHE) is a rare intermediate-grade vascular tumor characterized by a complex histologic component. It occasionally metastasizes, but local recurrence is not uncommon. CHE is mainly located in the extremities’ distal dermis and subcutaneous soft tissues. It is rarely located in the bone. We report here two cases originally occurred in bone. Case presentation The first case of CHE occurred solely on the left pubis. The second case is a patient post-resection of CHE in the manubrium sterni 10 years ago [1], who presented with multiple lesions in the left ilium and T6, T12 vertebra. All these lesions in the two cases showed osteolytic bone destruction on computed tomography (CT) scans and showed relatively high signal intensity on the fat-suppressed sequences of T2-weighted magnetic resonance (MR) images and isointense signal intensity on T1-weighted MR images. After injection of contrast agent (Gd-DTPA), the lesions showed inhomogeneous enhancement. 2-deoxy-2 [F-18] fluoro-D-glucose positron emission tomography-computed tomography (18FDG PET-CT) showed increased FDG uptake in these osteolytic bone destruction areas with SUVmax around 5.0. Both patients underwent surgery. Lesions in the left pubis and left ilium were confirmed by postoperative pathology while lesions on the vertebrae were only biopsied, not surgically resected. The first patient had no recurrence or metastasis in 5 years after surgery. The second patient had surgery recently and is still being followed up. Conclusions CHE occurring in bone is rarely reported. Our report provides more detailed information on the diagnosis of CHE. Given that CHE is locally aggressive and occasionally metastatic, PET-CT may be helpful in staging and follow-up.

show abstract

Erratum: Composite hemangioendothelioma with neuroendocrine marker expression: an aggressive variant

Abstract: In this article, the name of the second author is incorrect; the correct name is Alyaa Al-Ibraheemi.

Cited by 10 publications

References 0 publications

Application of the 3′ mRNA-Seq using unique molecular identifiers in highly degraded RNA derived from formalin-fixed, paraffin-embedded tissue

Application of the 3′ mRNA-Seq using unique molecular identifiers in highly degraded RNA derived from formalin-fixed, paraffin-embedded tissue

Malignant Conversion of Eyelid Capillary Hemangioma to Cutaneous Angiosarcoma

Composite hemangioendothelioma- report of two cases located in bone and review of the literature

Contact Info

Product

Resources

About