Erratum: Corrigendum: Evaluation of quantitative miRNA expression platforms in the microRNA quality control (miRQC) study

Mestdagh, Pieter; Hartmann, Nicole; Baeriswyl, Lukas; Andreasen, Ditte; Bernard, Nathalie; Chen, Caifu; Cheo, David L.; D'Andrade, Petula; DeMayo, Mike; Dennis, Lucas; Derveaux, Stefaan; Feng, Yun; Fulmer-Smentek, Stephanie; Gerstmayer, Bernhard; Gouffon, Julia S.; Grimley, Chris; Lader, Eric; Lee, Kathy; Luo, Shujun; Mouritzen, Peter; Narayanan, Aishwarya; Patel, Sunali; Peiffer, Sabine; Rüberg, Silvia; Schroth, Gary P.; Schuster, Dave; Shaffer, James; Shelton, Elliot J; Silveria, Scott; Ulmanella, Umberto; Veeramachaneni, Vamsi; Staedtler, Frank; Peters, Thomas; Guettouche, Toumy; Vandesompele, Jo

doi:10.1038/nmeth0914-971b

Cited by 4 publications

(1 citation statement)

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“…# 218073, Qiagen, Hilden, Germany) according to the manufacturer’s instructions. This system was reported as showing relatively high accuracy and high sensitivity among several quantification methods [ 16 ]. The following real-time PCR protocol was used: initial activation of HotStarTaq DNA Polymerase (from miScript kit; 95 °C, 15 min); 40 cycles of denaturation (94 °C, 15 s), annealing (55 °C, 30 s) and extension (70 °C, 34 s); and melting curve analysis.…”

Section: Methodsmentioning

confidence: 99%

Arginine Alters miRNA Expression Involved in Development and Proliferation of Rat Mammary Tissue

Zhou

et al. 2021

Animals

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This study was designed to determine the effects of dietary arginine on development and proliferation in rat mammary tissue through changes in miRNA profiles. Twelve pregnant Wistar rats were allocated randomly to two groups. A basal diet containing arginine or the control diet containing glutamate on an equal nitrogen basis as the arginine supplemented diet were used. The experiment included a pre-experimental period of four days before parturition and an experimental period of 17 days after parturition. Mammary tissue was collected for histology, RNA extraction and high-throughput sequencing analysis. The greater mammary acinar area indicated that arginine supplementation enhanced mammary tissue development (p < 0.01). MicroRNA profiling indicated that seven miRNA (miR-206-3p, miR-133a-5p, miR-133b-3p, miR-1-3p, miR-133a-3p, miR-1b and miR-486) were differentially expressed in response to Arginine when compared with the glutamate-based control group. In silico gene ontology enrichment and KEGG pathway analysis revealed between 240 and 535 putative target genes among the miRNA. Further verification by qPCR revealed concordance with the differential expression from the sequencing results: 17 of 28 target genes were differentially expressed (15 were highly expressed in arginine and 2 in control) and 11 target genes did not have significant difference in expression. In conclusion, our study suggests that arginine may potentially regulate the development of rat mammary glands through regulating miRNAs.

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Section: Methodsmentioning

confidence: 99%

Arginine Alters miRNA Expression Involved in Development and Proliferation of Rat Mammary Tissue

Zhou

et al. 2021

Animals

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Noncoding RNAs in Cancer Diagnosis

Zeng

2016

Advances in Experimental Medicine and Biology

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The accuracy and efficiency of tumor treatment depends mainly on early and precise diagnosis. Although histopathology is always the gold standard for cancer diagnosis, noninvasive biomarkers represent an opportunity for early detection and molecular staging of cancer. Besides the classical tumor markers, noncoding RNAs (ncRNAs) emerge to be a novel category of biomarker for cancer diagnosis since the dysregulation of ncRNAs is closely associated with the development and progression of human cancers such as liver, lung, breast, gastric, and other kinds of cancers. In this chapter, we will summarize the different types of ncRNAs in the diagnosis of major human cancers. In addition, we will introduce the recent advances in the detection and applications of circulating serum or plasma ncRNAs and non-blood fluid ncRNAs because the noninvasive body fluid-based assays are easy to examine for cancer diagnosis and monitoring.

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Mechanistic Actions of microRNAs in Diabetic Wound Healing

Petković

Sørensen

Leal

et al. 2020

Cells

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Wound healing is a complex biological process that is impaired under diabetes conditions. Chronic non-healing wounds in diabetes are some of the most expensive healthcare expenditures worldwide. Early diagnosis and efficacious treatment strategies are needed. microRNAs (miRNAs), a class of 18–25 nucleotide long RNAs, are important regulatory molecules involved in gene expression regulation and in the repression of translation, controlling protein expression in health and disease. Recently, miRNAs have emerged as critical players in impaired wound healing and could be targets for potential therapies for non-healing wounds. Here, we review and discuss the mechanistic background of miRNA actions in chronic wounds that can shed the light on their utilization as specific wound healing biomarkers.

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Erratum: Corrigendum: Evaluation of quantitative miRNA expression platforms in the microRNA quality control (miRQC) study

Cited by 4 publications

References 1 publication

Arginine Alters miRNA Expression Involved in Development and Proliferation of Rat Mammary Tissue

Arginine Alters miRNA Expression Involved in Development and Proliferation of Rat Mammary Tissue

Noncoding RNAs in Cancer Diagnosis

Mechanistic Actions of microRNAs in Diabetic Wound Healing

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