Erratum for Schooley et al., “Development and Use of Personalized Bacteriophage-Based Therapeutic Cocktails To Treat a Patient with a Disseminated Resistant Acinetobacter baumannii Infection”

Schooley, Robert T.; Biswas, Biswajit; Gill, Jason J.; Hernandez-Morales, Adriana; Lancaster, Jacob; Lessor, Lauren; Barr, Jeremy J.; Reed, Sharon L.; Rohwer, Forest; Benler, Sean; Segall, Anca M.; Taplitz, Randy; Smith, Davey M.; Kerr, Kim M.; Kumaraswamy, Monika; Nizet, Victor; Lin, Leo; McCauley, Melanie; Strathdee, Steffanie A.; Benson, Constance A.; Pope, Robert K.; Leroux, Brian M.; Picel, Andrew C.; Mateczun, Alfred; Cilwa, Katherine E.; Regeimbal, James; Estrella, Luis A.; Wolfe, David M.; Henry, Matthew; Quiñónes, Javier; Salka, Scott; Bishop‐Lilly, Kimberly A.; Young, Ralph H.; Hamilton, Theron

doi:10.1128/aac.02221-18

“…The vast potential of bacteriophages (phages) and their component parts as diagnostic and antibacterial tools is being realized in medicine (e.g., phage therapy) (Schooley et al, 2017;Skurnik et al, 2007), biotechnology (Karimi et al, 2016;Lee et al, 2012;O'Sullivan et al, 2016), and the food industry (Guenther et al, 2009;Schmelcher and Loessner, 2014). The advantage of using phages as antibacterial agents is their natural ability to infect and kill a specific range of bacteria within highly complex microbial populations.…”

Section: Introductionmentioning

confidence: 99%

Salmonella Phage S16 Tail Fiber Adhesin Features a Rare Polyglycine Rich Domain for Host Recognition

Dunne

¹

,

Denyes

²

,

Arndt

³

et al. 2018

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The ability of phages to infect specific bacteria has led to their exploitation as bio-tools for bacterial remediation and detection. Many phages recognize bacterial hosts via adhesin tips of their long tail fibers (LTFs). Adhesin sequence plasticity modulates receptor specificity, and thus primarily defines a phage's host range. Here we present the crystal structure of an adhesin (gp38) attached to a trimeric b-helical tip (gp37) from the Salmonella phage S16 LTF. Gp38 contains rare polyglycine type II helices folded into a packed lattice, herein designated ''PG II sandwich.'' Sequence variability within the domain is limited to surface-exposed helices and distal loops that form putative receptor-binding sites. In silico analyses revealed a prevalence of the adhesin architecture among T-even phages, excluding the archetypal T4 phage. Overall, S16 LTF provides a valuable model for understanding binding mechanisms of phage adhesins, and for engineering of phage adhesins with expandable or modulated host ranges.

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“…It is possible that shortly after its beginning, PT could have resulted in selection of phage resistant K. pneumoniae strain that required additional antibiotic application. Interestingly, a synergistic effect between meropenem and anti-Acinetobacter baumanii phage was recently reported [39].…”

Section: Therapy With Bacteriophages (Phage Therapy)mentioning

confidence: 97%

Treatment of recurrent urinary tract infections in a 60-year-old kidney transplant recipient – last-chance antibiotics and phage therapy

Rostkowska

¹

,

Międzybrodzki

²

,

Miszewska-Szyszkowska

³

et al. 2019

Preprint

0

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Background: The article underlines the problem of antimicrobial resistance in transplant departments where wide-spectrum antibiotics must often be used as first line treatment for immunocompromised patients. This applies especially to kidney transplant recipients who suffer from urinary tract infections. Additional means to control infections and support treatment methods are much needed in departments treating transplant patients. Case presentation: This article presents a case of a 60-year-old patient after kidney transplantation repeatedly admitted to the hospital due to recurrent urinary tract infections, including an episode of urosepsis, caused by Klebsiella pneumoniae producing extended-spectrum beta-lactamases (ESBL). Kidney transplantation was performed due to renal insufficiency caused by polycystic kidney disease, without removing of the patient’s own kidneys. As a consequence of numerous episodes of urinary tract infections (12 episodes within 15 months since transplantation), the patient developed chronic infection caused by ESBL-producing K. pneumoniae which showed variable susceptibility to carbapenems and full susceptibility to colistin only. In the meantime, the patient developed accompanying urinary tract infection caused by a vancomycin-resistant Enterococcus faecium and subsequent colonisation of the gastro-intestinal tract by this strain. In an attempt to curb the K. pneumoniae infection, bacteriophage therapy was applied on the experimental basis, coordinated by the Phage Therapy Unit of the Hirszfeld Institute in Wrocław, Poland. Probiotics were also used in an attempt to modify the bacterial gut colonisation. Eventually, the patient fully recovered following nephrectomy of his own left kidney in which cysts were suspected to be the reservoir of bacteria causing recurring infections. In this article we discuss some treatment methods complementary to classic antibiotic therapy, in times when antimicrobial resistance is on the rapid rise. Conclusions: It is necessary to further research methods of decreasing our reliance on antibiotics in view of falling susceptibility to those medicines. Phage therapy or altering patient’s microbiome can give us an edge in tackling infections which today are treated solely with antimicrobials. This would be of great significance to transplant departments.

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“…It conducts PT in outpatients based on the rules of a 'therapeutic experiment' pneumoniae strain that required additional antibiotic application. Interestingly, a synergistic effect between meropenem and anti-Acinetobacter baumanii phage was recently reported [39].…”

Section: Therapy With Bacteriophages (Phage Therapy)mentioning

confidence: 97%

Treatment of recurrent urinary tract infections in a 60-year-old kidney transplant recipient – last-chance antibiotics and phage therapy

Rostkowska

¹

,

Międzybrodzki

²

,

Miszewska-Szyszkowska

³

et al. 2019

Preprint

0

View full text Add to dashboard Cite

Background: The article underlines the problem of antimicrobial resistance in transplant departments where wide-spectrum antibiotics must often be used as first line treatment for immunocompromised patients. This applies especially to kidney transplant recipients who suffer from urinary tract infections. Additional means to control infections and support treatment methods are much needed in departments treating transplant patients. Case presentation: This article presents a case of a 60-year-old patient after kidney transplantation repeatedly admitted to the hospital due to recurrent urinary tract infections, including an episode of urosepsis, caused by Klebsiella pneumoniae producing extended-spectrum beta-lactamases (ESBL). Kidney transplantation was performed due to renal insufficiency caused by polycystic kidney disease, without removing of the patient’s own kidneys. As a consequence of numerous episodes of urinary tract infections (12 episodes within 15 months since transplantation), the patient developed chronic infection caused by ESBL-producing K. pneumoniae which showed variable susceptibility to carbapenems and full susceptibility to colistin only. In the meantime, the patient developed accompanying urinary tract infection caused by a vancomycin-resistant Enterococcus faecium and subsequent colonisation of the gastro-intestinal tract by this strain. In an attempt to curb the K. pneumoniae infection, bacteriophage therapy was applied on the experimental basis, coordinated by the Phage Therapy Unit of the Hirszfeld Institute in Wrocław, Poland. Probiotics were also used in an attempt to modify the bacterial gut colonisation. Eventually, the patient fully recovered following nephrectomy of his own left kidney in which cysts were suspected to be the reservoir of bacteria causing recurring infections. In this article we discuss some treatment methods complementary to classic antibiotic therapy, in times when antimicrobial resistance is on the rapid rise. Conclusions: It is necessary to further research methods of decreasing our reliance on antibiotics in view of falling susceptibility to those medicines. Phage therapy or altering patient’s microbiome can give us an edge in tackling infections which today are treated solely with antimicrobials. This would be of great significance to transplant departments.

show abstract

Erratum for Schooley et al., “Development and Use of Personalized Bacteriophage-Based Therapeutic Cocktails To Treat a Patient with a Disseminated Resistant Acinetobacter baumannii Infection”

Abstract: Erratum for Schooley et al., "Development and use of personalized bacteriophage-based therapeutic cocktails to treat a patient with a disseminated resistant Acinetobacter baumannii infection." Antimicrob Agents Chemother 62:e02221-18.

Cited by 27 publications

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Salmonella Phage S16 Tail Fiber Adhesin Features a Rare Polyglycine Rich Domain for Host Recognition

Salmonella Phage S16 Tail Fiber Adhesin Features a Rare Polyglycine Rich Domain for Host Recognition

Treatment of recurrent urinary tract infections in a 60-year-old kidney transplant recipient – last-chance antibiotics and phage therapy

Treatment of recurrent urinary tract infections in a 60-year-old kidney transplant recipient – last-chance antibiotics and phage therapy

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