Erratum to “Behavior and characteristics of biogenic amines, ornithine and lysine derivatized with the o-phthalaldehyde–ethanethiol–fluorenylmethyl chloroformate reagent” [J. Chromatogr. A 1087 (2005) 210–222]

Advancement in the derivatizations of the amino groups with the o-phthaldehyde-thiol and with the 9-fluorenylmethyloxycarbonyl chloride reagents

“…Thus, the best solution of the problem might has been the use of aged reagents. Unfortunately, the OPA/MPA, the OPA/NAC and the OPA/MCE reagents can be applied until 9 days, while the OPA/ET one until two days only: remaining on the safe side, in order to obtain quantitative derivatizations [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. …”

“…Before going into details it is worthy of mention that in the literature as model compounds of stability studies (from the beginning and thereafter continuously), glycine, GABA, ␤-alanine, histidine, ornithine and lysine have been selected as the less stable ones: in shortage of knowledge of their intrinsic characteristics, clarified recently [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. Since (i) these six AAs provide more than one OPA derivative, (ii) resulting in products of similar high stability as any other AAs: so long as, the total of their FL or UV responses, i.e., the sum of derivatives obtained from the given AA were taken into account in their quantitation.…”

“…Certainly these two derivatization protocols did have and they still have their uncertainties, limitations, shortages and advantages: however, in the last decade the overwhelming part of uncertainties were examined and cleared up: resulting in the trouble-free, conscious and reliable use the OPA [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] and that of the FMOC [20,21] techniques, as well.…”

“…In order to get answer to these questions in author's laboratory two approaches were followed: stoichiometric investigations were carried out with simultaneous PDA and FL detections [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] while structure elucidation were confirmed by on line HPLC/PDA/mass spectrometry (MS) applying electro spray ionization (ESI) in the positive mode [7,12,13,15]. The literature contains a variety of contradictory conditions regarding derivatization conditions.…”

“…; () = in parentheses percentages, expressed in the number of the total, given in the last two horizontal line. own experiences [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] the following questions had to be answered:…”