Erratum to “Magnetic attraction of iron-endocytosed corneal endothelial cells to Descemet's membrance”, by T. Mimura, N. Shimomura, T. Usui, Y. Noda, Y. Kaji, S. Yamagami, S. Amano, K. Miyata, M. Arie [Experimental Eye Research 76 (2003) 745–751]

Mimura, Tsuyoshi; Shimomura, Naoki; Usui, Tomohiro; Noda, Yasuo; Kaji, Yuichi; Yamagami, Satoru; Amano, Shiro; Araie, M

doi:10.1016/s0014-4835(03)00130-1

Cited by 12 publications

(20 citation statements)

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“…Some reports have also shown that suspension cultures of limbal stromal cells, under serum-free conditions, could generate spheres containing cells with neural potential and also very early progenitors expressing Oct4, Sox2, and Nanog [22,25,[27][28][29]. As shown in Figure 4B-4D, we observed self-organizing 3D spheres in midto late-stage suspension cultures, specifically originating at the leading edges or at junctions of adjacent epithelial holoclones that merge with each other.…”

Section: Self-organizing Niche-like 3d Compartments In Adherent Cultusupporting

confidence: 70%

Spatial Distribution of Niche and Stem Cells in Ex Vivo Human Limbal Cultures

Mariappan

Kacham

Purushotham

et al. 2014

Stem Cells Translational Medicine

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Stem cells at the limbus mediate corneal epithelial regeneration and regulate normal tissue homeostasis. Ex vivo cultured limbal epithelial transplantations are being widely practiced in the treatment of limbal stem cell deficiency. In this report, we examined whether the limbal niche cells that nurture and regulate epithelial stem cells coexist in ex vivo limbal cultures. We also compared the inherent differences between explant and suspension culture systems in terms of spatial distribution of niche cells and their effect on epithelial stem cell proliferation, migration, and differentiation in vitro. We report that the stem cell content of both culture systems was similar, explaining the comparable clinical outcomes reported using these two methods. We also showed that the niche cells get expanded in culture and the nestin-positive cells migrate at the leading edges to direct epithelial cell migration in suspension cultures, whereas they are limited to the intact niche in explant cultures. We provide evidence that C/EBPd-positive, p15-positive, and quiescent, label-retaining, early activated stem cells migrate at the leading edges to regulate epithelial cell proliferation in explant cultures, and this position effect is lost in early suspension cultures. However, in confluent suspension cultures, the stem cells and niche cells interact with each another, migrate in spiraling patterns, and self-organize to form three-dimensional niche-like compartments resembling the limbal crypts and thereby reestablish the position effect. These 3D-sphere clusters are enriched with nestin-, vimentin-, S100-, and p27-positive niche cells and p15-, p21-, p63a-, C/EBPd-, ABCG2-, and Pax6-positive quiescent epithelial stem cells. STEM CELLS

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Section: Self-organizing Niche-like 3d Compartments In Adherent Cultusupporting

confidence: 70%

Spatial Distribution of Niche and Stem Cells in Ex Vivo Human Limbal Cultures

Mariappan

Kacham

Purushotham

et al. 2014

Stem Cells Translational Medicine

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“…[2,5–8] In contrast to the stromal layers, tissue engineered corneal epithelium [9] and endothelium [10] have been successfully developed in animal models due to their relatively simple features. New approaches for engineering corneal equivalents have been proposed by using hydrogel scaffolds, [5,11,12] porous sponges, [13] fibers, [1] and films. [2,7] Recent efforts have focused on employing corneal fibro-blasts and ECM alignment toward reconstrued stroma.…”

Section: Introductionmentioning

confidence: 99%

“…[2,7] Recent efforts have focused on employing corneal fibro-blasts and ECM alignment toward reconstrued stroma. [1,2,7,11,12] The proper composition and well-aligned microstructure of the stroma determine the biomechanical and optical properties, thus detailed control of cell and ECM deposition become critical to the process.…”

Section: Introductionmentioning

confidence: 99%

Response of Human Corneal Fibroblasts on Silk Film Surface Patterns

Gil

Park

Marchant

et al. 2010

Macromolecular Bioscience

128

125

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Transparent, biodegradable, mechanically robust, and surface-patterned silk films were evaluated for the effect of surface morphology on human corneal fibroblast (hCF) cell proliferation, orientation, and ECM deposition and alignment. A series of dimensionally different surface groove patterns were prepared from optically graded glass substrates followed by casting poly(dimethylsiloxane) (PDMS) replica molds. The features on the patterned silk films showed an array of asymmetric triangles and displayed 37–342 nm depths and 445–3 582 nm widths. hCF DNA content on all patterned films were not significantly different from that on flat silk films after 4 d in culture. However, the depth and width of the grooves influenced cell alignment, while the depth differences affected cell orientation; overall, deeper and narrower grooves induced more hCF orientation. Over 14 d in culture, cell layers and actin filament organization demonstrated that confluent hCFs and their cytoskeletal filaments were oriented along the direction of the silk film patterned groove axis. Collagen type V and proteoglycans (decorin and biglycan), important markers of corneal stromal tissue, were highly expressed with alignment. Understanding corneal stromal fibroblast responses to surface features on a protein-based biomaterial applicable in vivo for corneal repair potential suggests options to improve corneal tissue mimics. Further, the approaches provide fundamental biomaterial designs useful for bioengineering oriented tissue layers, an endemic feature in most biological tissue structures that lead to critical tissue functions.

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“…The expression of VEGF-C/VEGFR-3 mRNA was dramatically up-regulated 3 days after the injury but gradually decreased. Importantly, VEGF-C was mainly detected in inflammatory cells, in comparison with VEGFR-3 expression in newly formed vessels in the corneal stroma [116]. After the penetrating keratoplasty, newly formed lymphatics occurred de novo within the stroma from limbal corneal area and extended into central area on 7 days, and then dimensionally established lymphatic connection [63,117].…”

Section: Inflamed Cornea (Penetrating Keratoplasty)mentioning

confidence: 96%

Lymphatic Endothelial Cells, Inflammatory Lymphangiogenesis, and Prospective Players

2007

CMC

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De novo lymphangiogenesis influences different pathological courses via modulating tissue fluid homeostasis, macromolecule absorption, and leukocyte transmigration. During the past decade, improved understanding of lymphatic biology, especially VEGF-C/-D/VEGFR-3-mediating lymphangiogenesis has substantially promoted clinical research in lymphatic insufficiency. The role of lymphangiogenesis in the setting of the inflammatory processes observed in transplants, inflamed cornea, wound healing, acquired lymphedema and tumor invasion, however, remains to be elucidated. The chemokine family of peptide chemoattractants and other mediators, e.g., CCL21-CCR7, D6, NF-kappaB and TNF-alpha may be important contributors to pathophysiological changes of lymphatic endothelial cells (LECs) and inflammatory lymphangiogenesis. Dendritic cells and macrophages may also involve in LEC proliferation and differentiation. Increased knowledge of LEC-specific modulators in inflammatory microenvironment is vital for prevention and treatment of lymphatic-associated diseases.

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Erratum to “Magnetic attraction of iron-endocytosed corneal endothelial cells to Descemet's membrance”, by T. Mimura, N. Shimomura, T. Usui, Y. Noda, Y. Kaji, S. Yamagami, S. Amano, K. Miyata, M. Arie [Experimental Eye Research 76 (2003) 745–751]

Cited by 12 publications

References 0 publications

Spatial Distribution of Niche and Stem Cells in Ex Vivo Human Limbal Cultures

Spatial Distribution of Niche and Stem Cells in Ex Vivo Human Limbal Cultures

Response of Human Corneal Fibroblasts on Silk Film Surface Patterns

Lymphatic Endothelial Cells, Inflammatory Lymphangiogenesis, and Prospective Players

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