Erratum to: Production of an oligosaccharide-specific cellobiohydrolase from the thermophilic fungus Thielavia terrestris

Woon, James Sy Keen; Mackeen, Muhammad Mukram Mohamed; Sudin, Amirul Husna; Mahadi, Nor Muhammad; Illias, Rosli Md.; Murad, Abdul Munir Abd.; Bakar, Farah Diba Abu

doi:10.1007/s10529-016-2065-8

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“…3B, which was similar to cellobiohydrolases from other thermophilic fungi, such as Fusarium chlamydosporum, Thermoascus aurantiacus and Thielavia terrestris. (Qin et al, 2010;Hong et al, 2003;Woon et al, 2016). The bivalent metallic cations Mg 2+ and Ca 2+ significantly enhanced the activity of CtCel6, while the monovalent cations K + and Na + were not detected with obvious effect (Table 1).…”

Section: Discussionmentioning

confidence: 96%

A Novel Application Potential of GH6 Cellobiohydrolase CtCel6 from Thermophilic Chaetomium thermophilum for Gene Cloning, Heterologous Expression and Biological Characterization

Zhou¹,

Jia²,

Ji³

et al. 2017

IJAB

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Chaetomium thermophilum is a thermophilic fungus expressed a series of glycoside hydrolases. Genome sequence analysis of C. thermophilium revealed that ctcel6 gene encoded a putative cellobiohydrolase which composed of 397 amino acid residues including a predicted signal peptide sequence. Ctcel6 gene was cloned, heterologously expressed in Pichia pastoris and purified by Ni 2+ affinity chromatography. Sequence alignment indicated that CtCel6 enzyme belonged to glycoside hydrolase family 6 (GH6) and the molecular mass of purified recombinant enzyme CtCel6 was 42 kDa by SDS-PAGE analysis. Characterization of recombinant CtCel6 exhibited high hydrolysis activity and excellent thermostability. The optimum reaction temperature and pH was 70 o C and pH 5, respectively. The bivalent metallic cations Mg 2+ and Ca 2+ significantly enhanced the activity of CtCel6. The specific activity of CtCel6 enzyme was 1.27 U/mg and Km value was 0.38 mM on β-Dglucan. The substrate specificity and hydrolysis products insisted that CtCel6 was an exo-/endo-type cellobiohydrolase. The biochemical properties of recombinant CtCel6 made it potentially effective for bioconversion of biomass and had tremendous potential in industrial applications such as enzyme preparation industry and feed processing industry.

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Section: Discussionmentioning

confidence: 96%

A Novel Application Potential of GH6 Cellobiohydrolase CtCel6 from Thermophilic Chaetomium thermophilum for Gene Cloning, Heterologous Expression and Biological Characterization

Zhou¹,

Jia²,

Ji³

et al. 2017

IJAB

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Erratum to: Production of an oligosaccharide-specific cellobiohydrolase from the thermophilic fungus Thielavia terrestris

Abstract: Unfortunately, Author name ''Mukram Mohamed Mackeen'' had been published incorrectly as ''Mukram MohamedMackeen'' without a space. This error has been rectified in the original article.

Cited by 1 publication

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A Novel Application Potential of GH6 Cellobiohydrolase CtCel6 from Thermophilic Chaetomium thermophilum for Gene Cloning, Heterologous Expression and Biological Characterization

A Novel Application Potential of GH6 Cellobiohydrolase CtCel6 from Thermophilic Chaetomium thermophilum for Gene Cloning, Heterologous Expression and Biological Characterization

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