Errors in Spectrophotometry and Colorimetry of Fluorescent Samples Caused by Polarization of Measuring System

Abstract

This article describes a method for the prediction of the reflected and the fluorescent spectral radiance factor which are expected to be measured by use of a measuring system with polarizing properties, and a method for the estimation of the spectrophotometric errors in the predicted radiance factors. Polarization properties are described for the measuring system, the white standard surface, and the two kinds of fluorescent samples used in the two‐monochromator method of the present study. In this method, the spectrophotometric errors in the reflected spectral radiance factor are estimated to be not less than 1% at some wavelengths. However, the error of the fluorescent spectral radiance factor is relatively small. Colorimetric errors of fluorescent samples due to polarization are estimated for the one‐ and two‐monochromator methods. The errors expressed as CIELUV color differences are estimated to be less than 1.0 unit and are negligibly small.

Estimation of bispectral Donaldson matrices of fluorescent objects by using two illuminant projections

“…On the other hand, the two-monochromator method is much more precise and direct for measuring the bispectral radiance factor, as filters are not needed. However, in this case, the polarization effect of the excitation monochromator may happen [11]. Essential problems of the two-monochromator method are that it is time-consuming and expensive, which is confined in the laboratory setup.…”