Erwinia soft rot resistance of potato cultivars expressing antimicrobial peptide tachyplesin I

Allefs, J.H.M.; Jong, E.R. de; Florack, D.E.A.; Hoogendoorn, J.; Stiekema, Willem J.

doi:10.1007/bf00441425

Cited by 44 publications

(15 citation statements)

References 19 publications

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“…Three clones with enhanced resistance were selected after 3 years of testing. Allefs et al [6] fused the sequence encoding a synthetic tachyplesin I gene with that of the barley hordothionin signal peptide. A low expression of this chimaeric gene in three potato cultivars revealed slight inhibitory effects to E. carotovora subsp.…”

Section: Expression Of Antimicrobial Proteinsmentioning

confidence: 99%

Improvement for Bacterial Wilt Resistance in Potato By Conventional and Biotechnological Approaches

2012

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Bacterial wilt (BW) of potato caused by the bacterium Ralstonia solanacearum (Rs) is considered a serious problem particularly in tropical, subtropical and warm temperate regions. Chemical-, cultural-and biological control of BW has limited success. Thus, the control of BW through resistance breeding and biotechnology is considered to be very important and necessary. Rs is considered a 'species complex' and has significant variation at physiological, serological and genetic levels. The bacterium has an unusually wide host range with over 400 hosts belonging to more than 50 botanical families. A large number of Solanum species have been screened for resistance to this bacterium, but so far no Solanum species has been found to have complete immunity. A high degree of resistance to Rs was found only in S. phureja, a diploid relative of cultivated tetraploid potatoes. The resistance has been transferred from S. phureja to cultivated potatoes through introgression breeding as well as somatic hybridization. Although moderate to highly resistant potato varieties have been released, high frequency of latent infection in tubers is still a major problem. Further, the resistant cultivars are not adapted to different agro-climatic zones and are not effective against all the strains of the pathogen. Biotechnological approaches involving the use of antimicrobial peptides, plant defence genes and plant resistance genes are being tried. This paper reviews the global situation with regard to screening of genetic resources and their utilization in resistance breeding for BW in potato and also the status and the opportunities that biotechnology offers to combat this disease.

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Section: Expression Of Antimicrobial Proteinsmentioning

confidence: 99%

Improvement for Bacterial Wilt Resistance in Potato By Conventional and Biotechnological Approaches

2012

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“…Additionally, it is a prerequisite that the product of the transferred gene does not compromise the growth and development of the intact plant. It has been reported that expression of small peptides in plants is an effective way to enhance resistance to plant pathogens (Allefs et al 1996;Cary et al 2000;Liang et al 2002;Alan et al 2004;Osusky et al 2004). However, this strategy is complicated by stability issues of the peptides.…”

Section: Introductionmentioning

confidence: 95%

Increased pathogen resistance and yield in transgenic plants expressing combinations of the modified antimicrobial peptides based on indolicidin and magainin

Xing

Lawrence

Chambers

et al. 2005

Planta

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Reverse peptide of indolicidin (Rev4), a 13-residue peptide based on the sequence of indolicidin, has been shown to possess both strong antimicrobial and protease inhibitory activities in vitro. To evaluate its efficacy in vivo, we produced and evaluated transgenic tobacco (Nicotiana tabacum L.) and Arabidopsis thaliana [(L.) Heynh.] plants expressing Rev4 with different signal peptide sequences for pathogen resistance. All transgenic plants showed normal growth and development, an indication of no or low cytotoxicity of the peptide. Furthermore, the transgenic plants exhibited elevated resistance to three bacterial and two oomycete pathogens. Interestingly, tobacco plants expressing Rev4 displayed enhanced yield compared to the control as indicated by an increased biomass production by as much as 34% in two field trials. When Rev4 was coexpressed with another antimicrobial peptide, Myp30, the disease resistance levels in the transgenic Arabidopsis were enhanced. These findings suggest the potential of using these peptides to protect plants from microbial pathogens and to enhance yield.

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“…These display increased antimicrobial activity against a broader spectrum of microorganisms and higher stability than their natural counterparts yet do not harm animal and plant cells in vitro (Chen et al 1988;Maloy et al 1990; hancing disease resistance in crop plants. Interest in the utilization of AMPs for the protection of economically important plants has led to the generation of transgenic plants expressing cecropins (Jaynes et al 1993;Hightower et al 1994;Allefs et al 1995;Florack et al 1995;Huang et al 1997;Osusky et al 2000;Liu et al 2001), D4E1 (Cary et al 2000), magainins (Smith et al 1998;Alan and Earle 1999;DeGray et al 2001;Li et al 2001;Liang et al 2002;Chakrabarti et al 2003), sarcotoxin IA (Ohshima et al 1999;Mitsuhara et al 2000), and tachyplesin I (Allefs et al 1996). In these studies effects of the AMP expression varied from none to high levels of disease resistance, depending on the genes and expression system used and the pathogenicity tests performed.…”

Section: Introductionmentioning

confidence: 98%

Expression of a magainin-type antimicrobial peptide gene (MSI-99) in tomato enhances resistance to bacterial speck disease

2004

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MSI-99 is a synthetic analog of magainin II (MII), a small cationic peptide highly inhibitory to a wide spectrum of microbial organisms. Tomato plants were transformed to express a gene encoding the MSI-99 peptide and tested for possible enhancement of resistance to important pathogens of this crop. Thirty-six tomato transformants carrying an MSI-99 expression vector designed to target the peptide into extracellular spaces were obtained by Agrobacterium tumefaciens-mediated transformation. Expression of MSI-99 caused no obvious cytotoxic effects in these plants. In the tests with Pseudomonas syringae pv. tomato (bacterial speck pathogen) at 10(5 )CFU/ml, several MSI-99-expressing lines developed significantly fewer disease symptoms than controls. However, MSI-99-expressing lines were not significantly different from controls in their responses to the fungal pathogen Alternaria solani (early blight) and the oomycete pathogen Phytophthora infestans (late blight). These findings are in accordance with our previous in vitro inhibition tests, which showed that the MSI-99 peptide is more inhibitory against bacteria than against fungi and oomycetes. Additional in vitro inhibition assays showed that MSI-99 loses its antimicrobial activity in the total or extracellular fluids from leaflets of non-transformed tomato plants; however, P. syringae pv. tomato could not multiply in the extracellular fluid from an MSI-99-expressing line. Our results suggest that expression strategies providing continuous high expression of MSI-99 will be necessary to achieve significant enhancement of plant disease resistance.

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Erwinia soft rot resistance of potato cultivars expressing antimicrobial peptide tachyplesin I

Cited by 44 publications

References 19 publications

Improvement for Bacterial Wilt Resistance in Potato By Conventional and Biotechnological Approaches

Improvement for Bacterial Wilt Resistance in Potato By Conventional and Biotechnological Approaches

Increased pathogen resistance and yield in transgenic plants expressing combinations of the modified antimicrobial peptides based on indolicidin and magainin

Expression of a magainin-type antimicrobial peptide gene (MSI-99) in tomato enhances resistance to bacterial speck disease

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