Erwinia wuhanensis sp. nov. isolated from human blood

Abstract

A novel Erwinia strain BC051422T was isolated from the blood of a patient at the Central Hospital of Wuhan, Wuhan, PR China in 2022. The strain was found to be Gram stain negative, facultatively anaerobic, motile, and rod-shaped. A preliminary analysis based on the 16S rRNA gene sequence and multi-locus sequence analysis of the atpD, infB, rpoB, and gyrB gene revealed that this strain is closely related to members of the genus Erwinia. The strain was subjected to whole genome sequencing and both average nucleotide identity (ANI) and in silico DNA–DNA hybridization (isDDH) values between strain BC051422T and type strains of all known species in Erwiniaceae were 68.8 to 83.4% and 19.2 to 35.5%, respectively, which are lower than the 95% (ANI) and 70% (isDDH) cut-off for delineation of bacterial species. The major cellular fatty acids of strain BC051422T is C16:0, C16:1 ω7c/C16:1 ω6c, and C17: 0 cyclo, which are similar to other Erwinia species. The genomic DNA G + C content of strain was 55.95 mol%. Strain BC051422T can be differentiated from other Erwinia species by its ability to ferment sucrose, but is negative for mannose, rhamnose, melibiose, and sorbitol. Genotypic and phenotypic characteristics indicate that strain BC051422T represent a novel species of the genus Erwinia, for which the names Erwinia wuhanensis sp. nov. is proposed. The type strain of Erwinia wuhanensis sp. nov. is BC051422T (= GDMCC 1.4074T = JCM 36319T).