Erxian herbal pair enhances bone formation in infected bone nonunion models and attenuates lipopolysaccharide-induced osteoblastinhibition by regulating miRNA-34a-5p

Zhang, Li; Zhang, Yang; Miao, Maomao; Hu, Shaoqi; Wang, Xuping; Zhao, Lisha; Huang, Xiaowen; Cao, Gang; Shou, Dan

doi:10.1080/21655979.2022.2085388

Cited by 3 publications

(2 citation statements)

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“…Our group has previously demonstrated that the miR‐34a‐5p/SMAD2 signalling axis can enhances bone formation in infected bone nonunion models and attenuates lipopolysaccharide‐induced osteoblast inhibition. 17 Researches showed that Long non‐coding RNAs could competitively bind to miRNAs, indirectly regulating the expression of miRNA target genes due to their sponge‐like effect. Among these, LncRNA Metastasis Associated in Lung Denocarcinoma Transcript 1 (LncRNA MALAT1), one of the few highly conserved nuclear lncRNAs, has been proved to regulate a variety of physiological processes.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, some of non‐coding RNAs (ncRNAs) were confirmed to be upstream regulators of TGF‐β/SMADs signalling pathway, 16 including miR‐34a‐5p, which associated with an inflammatory response in osteoblasts. Our group has previously demonstrated that the miR‐34a‐5p/SMAD2 signalling axis can enhances bone formation in infected bone nonunion models and attenuates lipopolysaccharide‐induced osteoblast inhibition 17 . Researches showed that Long non‐coding RNAs could competitively bind to miRNAs, indirectly regulating the expression of miRNA target genes due to their sponge‐like effect.…”

Section: Introductionmentioning

confidence: 99%

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Epimedium‐Curculigo herb pair enhances bone repair with infected bone defects and regulates osteoblasts through LncRNA MALAT1/miR‐34a‐5p/SMAD2 axis

Miao,

Li,

Sheng

et al. 2024

J Cellular Molecular Medi

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Infected bone defects (IBDs) are the common condition in the clinical practice of orthopaedics. Although surgery and anti‐infective medicine are the firstly chosen treatments, in many cases, patients experience a prolonged bone union process after anti‐infective treatment. Epimedium‐Curculigo herb pair (ECP) has been proved to be effective for bone repair. However, the mechanisms of ECP in IBDs are insufficiency. In this study, Effect of ECP in IBDs was verified by micro‐CT and histological examination. Qualitative and quantitative analysis of the main components in ECP containing medicated serum (ECP‐CS) were performed. The network pharmacological approaches were then applied to predict potential pathways for ECP associated with bone repair. In addition, the mechanism of ECP regulating LncRNA MALAT1/miRNA‐34a‐5p/SMAD2 signalling axis was evaluated by molecular biology experiments. In vivo experiments indicated that ECP could significantly promote bone repair. The results of the chemical components analysis and the pathway identification revealed that TGF‐β signalling pathway was related to ECP. The results of in vitro experiments indicated that ECP‐CS could reverse the damage caused by LPS through inhibiting the expressions of LncRNA MALAT1 and SMAD2, and improving the expressions of miR‐34a‐5p, ALP, RUNX2 and Collagen type І in osteoblasts significantly. This research showed that ECP could regulate the TGF‐β/SMADs signalling pathway to promote bone repair. Meanwhile, ECP could alleviate LPS‐induced bone loss by modulating the signalling axis of LncRNA MALAT1/miRNA‐34a‐5p/ SMAD2 in IBDs.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Epimedium‐Curculigo herb pair enhances bone repair with infected bone defects and regulates osteoblasts through LncRNA MALAT1/miR‐34a‐5p/SMAD2 axis

Miao,

Li,

Sheng

et al. 2024

J Cellular Molecular Medi

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Herb couplet medicines (Erxian) protect osteoblasts from high glucose-induced damage by reducing cell apoptosis in diabetic osteoporosis: A network pharmacology and experimental verification-based study

Zhou,

Li,

Zhang

et al. 2024

European Journal of Integrative Medicine

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Regulation of ferroptosis in osteoarthritis and osteoarthritic chondrocytes by typical MicroRNAs in chondrocytes

Yu,

Xiao,

Guan

et al. 2024

Front. Med.

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Osteoarthritis (OA) is a progressive degenerative disorder impacting bones and joints, worsened by chronic inflammation, immune dysregulation, mechanical stress, metabolic disturbances, and various other contributing factors. The complex interplay of cartilage damage, loss, and impaired repair mechanisms remains a critical and formidable aspect of OA pathogenesis. At the genetic level, multiple genes have been implicated in the modulation of chondrocyte metabolism, displaying both promotive and inhibitory roles. Recent research has increasingly focused on the influence of non-coding RNAs in the regulation of distinct cell types within bone tissue in OA. In particular, an expanding body of evidence highlights the regulatory roles of microRNAs in OA chondrocytes. This review aims to consolidate the most relevant microRNAs associated with OA chondrocytes, as identified in recent studies, and to elucidate their involvement in chondrocyte metabolic processes and ferroptosis. Furthermore, this study explores the complex regulatory interactions between long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) in OA, with an emphasis on microRNA-mediated mechanisms. Finally, critical gaps in the current research are identified, offering strategic insights to advance the understanding of OA pathophysiology and guide therapeutic developments in this field.

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Erxian herbal pair enhances bone formation in infected bone nonunion models and attenuates lipopolysaccharide-induced osteoblastinhibition by regulating miRNA-34a-5p

Cited by 3 publications

References 50 publications

Epimedium‐Curculigo herb pair enhances bone repair with infected bone defects and regulates osteoblasts through LncRNA MALAT1/miR‐34a‐5p/SMAD2 axis

Epimedium‐Curculigo herb pair enhances bone repair with infected bone defects and regulates osteoblasts through LncRNA MALAT1/miR‐34a‐5p/SMAD2 axis

Herb couplet medicines (Erxian) protect osteoblasts from high glucose-induced damage by reducing cell apoptosis in diabetic osteoporosis: A network pharmacology and experimental verification-based study

Regulation of ferroptosis in osteoarthritis and osteoarthritic chondrocytes by typical MicroRNAs in chondrocytes

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