Erythema Chronicum Migrans in Sweden: Clinical Manifestations and Antibodies to Ixodes ricinus Spirochete Measured by Indirect Immunofluorescence and Enzyme-linked Immunosorbent Assay

Stiernstedt, Göran; Eriksson, G; Enfors, Wera; Jörbeck, H; Svenungsson, Bo; Sköldenberg, Birgit; Granström, Marta

doi:10.3109/00365548609032330

Cited by 28 publications

(11 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The low sensitivity of the Diagast HA in both early and late Lyme borreliosis (8 % and 62 %) and the relatively low sensitivity of the IFAs in early Lyme borreliosis compared to the EIAs (31% versus ~ 46 %), is in agreement with the findings of others (19,(21)(22)(23)(24).…”

Section: Discussionsupporting

confidence: 90%

Evaluation of nine serological tests for diagnosis of Lyme borreliosis

Nohlmans

Blaauw

Bogaard

et al. 1994

Eur. J. Clin. Microbiol. Infect. Dis.

View full text Add to dashboard Cite

Two hundred serum specimens including 13 sera from patients with early Lyme borreliosis, 21 patients with late Lyme borreliosis, 15 rheumatoid factor positive sera, 31 sera from patients with syphilis and 84 sera from healthy controls were used to evaluate the following assays for the detection of antibodies to Borrelia burgdorferi: two in-house enzyme immunoassays (EIAs), two in-house immunofluorescent antibody assays (IFAs), a commercial haemagglutination assay (HA) (Diagast) and four commercial EIAs (Diagast, Dako, Diamedix, Whittaker Bioproducts). In early and late Lyme borreliosis sera sensitivity ranged from 8% to 62% and from 62% to 86% respectively. With the exception of the Dako EIA, which was signifcantly more sensitive in early Lyme borreliosis (62%) than the Diagast HA (8%) (p = 0.05), differences in sensitivity were not significant. In healthy controls the specificity was > or = 95% for all tests. Taking into account sensitivity, specificity, intra-test and inter-test precision, ease of performance and cost, the Dako EIA and Diamedix EIA were shown to be good alternatives to the in-house EIA and in-house IFA. Because of its low sensitivity in diagnosis of both early and late Lyme borreliosis, use of the Diagast HA should be discouraged.

show abstract

Section: Discussionsupporting

confidence: 90%

Evaluation of nine serological tests for diagnosis of Lyme borreliosis

Nohlmans

Blaauw

Bogaard

et al. 1994

Eur. J. Clin. Microbiol. Infect. Dis.

View full text Add to dashboard Cite

show abstract

“…Serological studies have shown seropositivity rates of 25 to 50% in patients with EM and 50 to 80% in patients with neuroborreliosis (9,10,14,21,22). The diagnostic sensitivity of ELISA has been increased by the introduction of new antigens, e.g., the flagellum antigen (10,14), and capture ELISA instead of indirect ELISA for the immunoglobulin M (IgM) analysis (12).…”

mentioning

confidence: 99%

Serological follow-up after treatment of patients with erythema migrans and neuroborreliosis

et al. 1994

View full text Add to dashboard Cite

To investigate the duration and kinetics of immunoglobulin M (IgM) and IgG antibodies against Borrelia burgdorferi in serum after treatment of Lyme borreliosis, consecutive serum samples from 30 seropositive patients with erythema migrans and 91 seropositive patients with neuroborreliosis were analyzed with a capture IgM enzyme-linked immunosorbent assay (ELISA) and an indirect IgG ELISA, both using B. burgdorferi flagella as the antigen. All the patients improved after treatment: 97 patients had a complete clinical recovery, while 24 patients had sequelae. The results showed that patients with erythema migrans and early neuroborreliosis more often initially had highly elevated IgM optical density (OD) values and low IgG OD values against B. burgdorferi, while the opposite was found in patients with late neuroborreliosis. During follow-up, the majority of patients had developed negative or significantly declining IgM ODs after 1 to 1.5 years but persistently positive IgM ODs were found up to 17 months after treatment of erythema migrans and 3 years after treatment of neuroborreliosis. IgG antibody levels declined more slowly and remained elevated to a larger extent, but more than half of the patients had developed negative IgG ODs within 5 years after therapy. However, positive IgG OD values were found after 9 to 10 years for patients treated for neuroborreliosis as well as erythema migrans. Both IgM and IgG antibodies against B. burgdorferi may persist for months to years after successful treatment of Lyme borreliosis. Consequently, a single serum sample with antibodies against B. burgdorferi must always be carefully evaluated and correlated to clinical symptoms.

show abstract

“…It should immediately be confirmed by determination of antibodies against B. burgdorferi (6)(7)(8)(9). In atypical patients serology is essential, with the reservation that a positive titer to B. burgdorferi may represent a "serologic scar" due to a previous infection without actual interest.…”

mentioning

confidence: 99%

Immunoblot Analysis of Antibody Binding to Polypeptides of Borrelia burgdorferi in Children with Different Clinical Manifestations of Lyme Disease

1989

View full text Add to dashboard Cite

ABSTRACT. We analyzed by means of immunoblot technique the patterns of antibodies binding to polypeptides of Borrelia burgdorferi B31 in the sera of 21 children with different stages of Lyme disease. All sera but one recognized the flagellar protein 41 kD and all but two the 83-kD protein. The number of proteins recognized rose from clinical stage I to stage 111. The polypeptides of the mol wt 55 and 31 kD were exclusively bound by IgM and the proteins 66, 58, 39, and 36 kD exclusively by IgG. Based on the number of proteins visualized by single sera, IgM was the predominant isotype in stages I and I1 peaking in stage 11, whereas in stage I11 IgG predominated. Considering the number of proteins recognized and the corresponding antibody isotype, a serologic differentiation between the three stages of the disease is feasible: within stage I and within stage I11 patients with different clinical signs had distinct antibody patterns. No clearcut pattern could be discriminated in stage I1 for patients with different settings. Immunoblotting yields a possible distinction between active infection and serological scar by the detection of specific antibody patterns. (Pediatr Res 26:377-382, 1989)

show abstract

Erythema Chronicum Migrans in Sweden: Clinical Manifestations and Antibodies to Ixodes ricinus Spirochete Measured by Indirect Immunofluorescence and Enzyme-linked Immunosorbent Assay

Cited by 28 publications

References 18 publications

Evaluation of nine serological tests for diagnosis of Lyme borreliosis

Evaluation of nine serological tests for diagnosis of Lyme borreliosis

Serological follow-up after treatment of patients with erythema migrans and neuroborreliosis

Immunoblot Analysis of Antibody Binding to Polypeptides of Borrelia burgdorferi in Children with Different Clinical Manifestations of Lyme Disease

Contact Info

Product

Resources

About