Erythritol production by Yarrowia lipolytica mutant strain M53 generated through atmospheric and room temperature plasma mutagenesis

Liu, Xiaoyan; Lv, Jinshun; Xu, Jiaxing; Xia, Jun; Dai, Benlin; Xu, Xiangqian; Xu, Jiming

doi:10.1007/s10068-017-0116-0

Cited by 26 publications

(19 citation statements)

References 36 publications

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“…Cells of Y. lipolytica SWJ‐1b were treated using atmospheric and room temperature plasma method (helium gas flow rate: QHe = 10 standard liters per minute, radio frequency power: 120 W, treatment time: 2 min), and the obtained mutants were then screened for high erythritol production strains. Y. lipolytica M53 could produce 0.65 g/g glycerol of erythritol or 0.54 g/g glycerol of citric acid respectively under different culture condition and was selected as the research strain . M53 was grown on yeast peptone dextrose agar slant with 10.0 g/L of yeast extract, 20.0 g/L of peptone, 20.0 g/L of glucose, and 20.0 g/L of agar.…”

Section: Methodsmentioning

confidence: 99%

Effects of osmotic pressure and pH on citric acid and erythritol production from waste cooking oil by Yarrowia lipolytica

Liu

et al. 2018

Engineering in Life Sciences

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Erythritol and citric acid could be produced from waste cooking oil (WCO) by Yarrowia lipolytica under different medium conditions, and osmotic pressure together with pH were considered to be the critical factors in this process. High osmotic pressure (2.76 osmol/L) combined with low pH (pH 3.0) promoted the highest yield of erythritol (21.8 g/L) accompanied by low‐producing citric acid (2.5 g/L). By contrast, the highest citric acid biosynthesis (12.6 g/L) was detected under a pH of 6.0 and an osmotic pressure of 0.75 osmol/L, when only 4.0 g/L of erythritol was yielded. Moreover, lipase activities in these two media were also detected, and pH 3.0–OP 2.76 was supposed to be more beneficial to lipase activity. Biochemical pathways involved in the biosynthesis of erythritol and citric acid were subsequently investigated, and the products yielded from WCO were assumed to be correlated with the activities of transketolase, erythrose reductase, citrate synthase, and glycerol kinase. However, RT‐PCR analysis revealed that mRNA levels of these enzymes did not significantly differ, confirming that metabolic flux regulations of erythritol and citric acid mostly took place at the post‐transcriptional level.

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Section: Methodsmentioning

confidence: 99%

Effects of osmotic pressure and pH on citric acid and erythritol production from waste cooking oil by Yarrowia lipolytica

Liu

et al. 2018

Engineering in Life Sciences

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“…Beside this, atmospheric and room temperature plasma mutagenesis was also used to generate mutants of Y. lipolytica with increased EOL productivity. With such a mutant strain (M53), glycerol fed-batch cultivation in a 5 L bioreactor led to an EOL titer of 169.3 g/L within 168 h with a yield of 0.65 g/g [108]. Figure 2.…”

Section: Erythritolmentioning

confidence: 99%

“…Fermented okara is a soybean residue, which was used as a feedstock for the production of EOL by mutant strain M53. This allowed an EOL titer of 14.7 g/L with a yield of 0.49 g/g in a 5 L bioreactor within 144 h [108]. By overexpressing SUC2 genes from Saccharomyces cerevisiae encoding invertase and native GUT1 gene (YALI0F00384g), encoding glycerol kinase (GK), EOL was produced from raw industrial molasses (60 g/L) and crude glycerol (100 g/L) and reached a titer of 100 g/L with a productivity and yield of 1.1 g/(L•h) and a yield of 0.67 g/g [109].…”

Section: Erythritolmentioning

confidence: 99%

Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We?

2020

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Sugar alcohols and organic acids that derive from the metabolism of certain microorganisms have a panoply of applications in agro-food, chemical and pharmaceutical industries. The main challenge in their production is to reach a productivity threshold that allow the process to be profitable. This relies on the construction of efficient cell factories by metabolic engineering and on the development of low-cost production processes by using industrial wastes or cheap and widely available raw materials as feedstock. The non-conventional yeast Yarrowia lipolytica has emerged recently as a potential producer of such metabolites owing its low nutritive requirements, its ability to grow at high cell densities in a bioreactor and ease of genome edition. This review will focus on current knowledge on the synthesis of the most important sugar alcohols and organic acids in Y. lipolytica.

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“…For example, Zhao et al utilized plasma discharge to mutate a polyunsaturated fatty acids producing strain of Schizochytrium sp [ 40 ]. Liu et al obtained a Yarrowia lipolytica M53 strain with high glycosyl alcohol content by plasma discharge mutagenesis [ 41 ]. Li et al showed that after Aspergillus terreus was treated with ARTP mutagenesis, it reached a higher itaconic acid yield of 19.3 g/L with 36.01% sugar conversion [ 42 ].…”

Section: Introductionmentioning

confidence: 99%

Improved production of polysaccharides in Ganoderma lingzhi mycelia by plasma mutagenesis and rapid screening of mutated strains through infrared spectroscopy

Zhang

et al. 2018

PLoS ONE

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As a traditional Chinese medicine, Ganoderma lingzhi has attracted increasing attention for both scientific research and medical application. In this work, in order to improve the production of polysaccharides from an original wide-type (WT) strain (named "RWY-0") of Ganoderma lingzhi, we applied atmospheric-pressure dielectric barrier discharge (DBD) nonthermal plasma to the protoplasts of RWY-0 for mutagenesis treatment. Through a randomly amplified polymorphic DNA (RAPD) assay, at least 10 mutagenic strains were confirmed. They also showed different mycelium characteristics in terms of shape, color, size and biomass in liquid fermentation. The mutant strains were examined by infrared spectroscopy, and based on the established near-infrared (NIR) quantification model, the polysaccharide contents in these mutants were quantitatively evaluated. As a result, we found that the Ganoderma polysaccharide contents in some of the mutant strains were significantly changed compared with that of the original WT strain. The polysaccharide content of RWY-1 G. lingzhi was considerably higher than that of the WT strain, with an increase of 25.6%. Thus, this preliminary work demonstrates the extension of the plasma mutagenesis application in acquiring polysaccharide-enhanced Ganoderma lingzhi mutants and shows the usefulness of NIR spectroscopy in the rapid screening of mutagenic strains for other important ingredients.

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Erythritol production by Yarrowia lipolytica mutant strain M53 generated through atmospheric and room temperature plasma mutagenesis

Cited by 26 publications

References 36 publications

Effects of osmotic pressure and pH on citric acid and erythritol production from waste cooking oil by Yarrowia lipolytica

Effects of osmotic pressure and pH on citric acid and erythritol production from waste cooking oil by Yarrowia lipolytica

Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We?

Improved production of polysaccharides in Ganoderma lingzhi mycelia by plasma mutagenesis and rapid screening of mutated strains through infrared spectroscopy

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