Erythrobacter odishensis sp. nov. and Pontibacter odishensis sp. nov. isolated from dry soil of a solar saltern

Abstract

Two bacterial strains (JC130T and JA747T) were isolated from dry soils of a solar saltern. Phylogenetic analysis showed that strain JA747T clustered with species of the genus Erythrobacter belonging to the family Erythrobacteraceae of the class Alphaproteobacteria in the phylum Proteobacteria , while strain JC130T clustered with species of the genus Pontibacter belonging to the family Cytophagaceae of the phylum Bacteroidetes . Based on 16S rRNA gene sequence analysis, strain JA747T had highest similarity with Erythrobacter gangjinensis K7-2T (96.7 %) and other members of the genus Erythrobacter (<96 %). Strain JC130T had highest sequence similarity with Pontibacter korlensis X14-1T (98.1 %), Pontibacter actiniarum KMM 6156T (96.9 %) and other members of the genus Pontibacter (<96 %). However, strain JC130T showed less than 32 % DNA reassociation value (based on DNA–DNA hybridization) with Pontibacter korlensis NRRL B-51097T ( = X14-1T) and Pontibacter actiniarum LMG 23027T ( = KMM 6156T). Strain JA747T was positive for catalase and oxidase activity and negative for nitrate reduction, and hydrolysis of starch and casein. Phosphatidylglycerol, diphostatidylglycerol, phosphatidylethanolamine and phosphatidylcholine were the major polar lipids for strain JA747T. C18 : 1ω7c, C16 : 1ω6c/C16 : 1ω7c, C17 : 1ω6c and C16 : 0 were the major fatty acids of strain JA747T. Strain JC130T was positive for catalase and oxidase, and negative for gelatinase and nitrate reduction. Phosphatidylethanolamine was the major polar lipid of strain JC130T. Major fatty acids of strain JC130T were iso-C15 : 0 and summed feature 4 (anteiso-C17 : 1B/iso I). Based on the phenotypic, chemotaxonomic and molecular evidence presented, strains JA747T and JC130T are considered to represent two novel species of the genera Erythrobacter and Pontibacter , for which the names Erythrobacter odishensis sp. nov. (type strain JA747T = KCTC 23981T = NBRC 108930T) and Pontibacter odishensis sp. nov. (type strain JC130T = KCTC 23982T = LMG 26962T), respectively, are proposed. Emended descriptions of the genera Erythrobacter and Pontibacter are provided.

Genomic-based taxonomic classification of the family Erythrobacteraceae

“…The family Erythrobacteraceae , belonging to the order Sphingomonadales , class Alphaproteobacteria [1], is distributed globally, inhabiting various environments including subterrestrial, lake, intertidal areas, mangrove, coastal and deep-sea sediments [2–10], soil [11–13], desert sands [14, 15], a stadium seat [16], seawater [17–19], estuary water [20–22], fresh water [23, 24], hot springs [25–27], air [28] as well as plants and animals [29–36] (Table S1, available in the online version of this article). The members of the family Erythrobacteraceae are Gram-stain-negative, rod or pleomorphic coccoid-shaped, pink-, red-, orange- or yellow-pigmented, and aerobic chemoorganotrophs [1].…”

Rhodobacter sediminis sp. nov., isolated from lagoon sediments

“…Cellular fatty acids of photoheterotrophically grown cultures were analysed as described by Sasser (1990). Polar lipid analyses were performed using 1 g freeze-dried cells of photoheterotrophically grown cultures as described previously (Subhash et al, 2013b, c;Oren et al, 1996). Quinone analysis was performed after extraction with chloroform and methanol (2 : 1, v/v) by previously described methods (Imhoff, 1984;.…”

Spirochaeta lutea sp. nov., isolated from marine habitats and emended description of the genus Spirochaeta

“…16S rRNA gene amplification and sequencing was done as described previously [22]. Identification of the phylogenetic neighbours and calculation of pair wise 16S rRNA gene sequence similarity was done on the EzTaxon server [10].…”

“…pH tolerance range was examined by using buffered medium as described previously [21]. Growth on organic substrates (d-glucose, dgalactose, d-fructose, cellobiose, d-Mannitol, starch, l-rhamnose, d-lactose, d-mannose, d-sorbitol, pyruvate, l-glutamate, l-aspartic acid, fumarate, ethanol, glycerol, succinate, and benzoate) was tested in a minimal medium described by Subhash et al [22]. Vitamin requirements were tested by replacing yeast extract with single and also combinations of vitamins as growth factors.…”