Erythrocyte 2,3-Diphosphogly cerate, Po250%, and Available Oxygen in Young Rabbits with and without Postnatal Fall in Hemoglobin

Holter, P. H.; Halvorsen, S; Refsum, H. E.

doi:10.1203/00006450-198402000-00008

Cited by 10 publications

(8 citation statements)

References 12 publications

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“…The fact that these factors do not act independently of each other, the complete lack of corresponding relationship between 2,3-DPG and HbF in the whole population although FF 2,3-DPG and P 50 had similar patterns in both groups, indicate that the significantly increased concentration of 2,3-DPG seen in EPO recipients compared to controls, improves oxygen delivery and available O 2 and gives another advantage to rHu-EPO on top of that of enhanced erythropoiesis. We did not find changes in 2,3-DPG levels, before and after transfusions, in both groups; this demonstrates that the rise in 2,3-DPG is independent of variations in Hb [32].…”

Section: Discussionmentioning

confidence: 64%

Use of Erythropoietin and Its Effects on Blood Lactate and 2,3-Diphosphoglycerate in Premature Neonates

Soubasi¹,

Kremenopoulos²,

Tsantali

et al. 2000

Neonatology

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The aim of this study was to investigate the effect of recombinant human erythropoietin (rHu-EPO) on oxygen affinity and adequate oxygen delivery to the tissues of stable premature infants. 36 very-low-birth-weight infants were randomly assigned to either receive rHu-EPO (200 units/kg every other day) or not, and both groups were supplemented with iron, folic acid and vitamin E. Arterial blood gases, oxygen saturation, complete blood counts, fetal haemoglobin, 2,3-diphosphoglycerate (2,3-DPG) and blood lactate were analysed weekly, from the 1st week till discharge. Patients in the two groups were comparable. There was a trend in increasing lactate values towards the 4th to 5th weeks of life, which did not reach statistical significance. There was no correlation between lactate values and the studied variables (pH, BE, oxygen saturation). In 35 transfusions, pre- and 24 h post-transfusion blood lactate status was studied. In 23 of them, a decrease in post-transfusion lactate was noticed, whilst an increased post-transfusion level was shown in 10 cases and no change in 2 cases. The mean pre-transfusion lactate value was significantly higher than the post-transfusion one (24.04 ± 11.9 mg/dl before and 16.27 ± 8.5 mg/dl after transfusion; p = 0.0025). In both groups there was a steady rise in 2,3-DPG concentration over the period of study, and the 2,3-DPG values at the end of our study were significantly increased in the rHu-EPO group (rHu-EPO 5.98 ± 0.9, control 4.84 ± 0.7; p = 0.04). In conclusion, the use of rHu-EPO did not affect blood lactate levels compared to the control group. Regarding oxygen affinity, it seems that rHu-EPO causes a shift of the oxy-haemoglobin dissociation curve to the right. This is a previously unreported effect of rHu-EPO and its clinical use may, thus, confer to preterm babies an added advantage.

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Section: Discussionmentioning

confidence: 64%

Use of Erythropoietin and Its Effects on Blood Lactate and 2,3-Diphosphoglycerate in Premature Neonates

Soubasi¹,

Kremenopoulos²,

Tsantali

et al. 2000

Neonatology

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“…This may indicate that the marked rise in 2,3-DPG and the consequent shift of the O2 Hb dissociation curve to the right which regularly take place at this age (10,15), were sufficient to compensate for the fall in Hb and prevent tissue hypoxia on the 22nd day, but not around the nadir of the anemia. However, the difference in estimated O2 delivery capacity of the blood from the 22nd and to the 29th day was probably small, and the marked increase in plasma ESF may also reflect that the regions governing erythropoietin production/release became increasingly sensitive to low O2 tension at this stage of development (10).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that the marked postnatal rise in erythrocyte 2,3-DPG and blood POz 50% in rabbit is independent of Hb (15,16). Thus, the 0 2 delivery capacity of the blood in the iron-treated rabbits must have increased markedly in the course of the observation period (1 5).…”

Section: Discussionmentioning

confidence: 99%

“…The possibility also exists that the increase in plasma ESF only in part is due to hypoxic stimulation, and that other, hypoxia-independent mechanisms play a role for the regulation of erythropoiesis at this age. The fact that the postnatal fall in Hb in rabbits can be avoided by parenteral iron supplements [14,15) makes it possible to distinguish between anemic-hypoxia dependent and independent regulating mechanisms.…”

mentioning

confidence: 99%

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Regulation of Erythropoiesis in Suckling Rabbits with and without Postnatal Anemia: Partial Suppression of Production/Release of Erythropoiesis Stimulating Factor(s) by Iron Supplements

et al. 1987

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“…Animal care, anaesthesia and blood sampling were performed as previously (Holter et al 1984, Holter & Refsum 1985. Newborn (age 12-24 h) and adult Dutch Belted or Chinchilla rabbits were used for the studies.…”

Section: A T E R I a L S And M E T H O D Smentioning

confidence: 99%

Haemoglobin O₂ binding in newborn and adult rabbits

Holter

Kierulf

Refsum

1987

Acta Physiologica Scandinavica

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The relationship between haemoglobin O2 saturation and 2,3-diphosphoglycerate concentration (2,3-DPG) in haemoglobin solutions equilibrated with 3.7% O2-5.7% CO2 were identical in newborn and adult rabbits over a wide range of 2,3-DPG (0.5-40.0 mumol g-1 Hb), and independent of the haemoglobin concentration (1-10 g dl-1). Also, the resistance of the haemoglobin to alkali denaturation and the spectrophotometric absorption spectra of carboxy and oxyhaemoglobin between 500 nm and 650 nm, as well as the isoelectric focusing pattern of the haemoglobin after early and late stabilization with CO, were the same in newborn and adult animals. Both demonstrated one dominant band, focusing at pH 7.17, and one less conspicuous at pH 7.40. The erythrocyte pH was higher in the newborn than in the adult animals. Isoelectric focusing of erythrocyte pyruvate kinase demonstrated two isoenzyme bands in the newborn animals, one dominant fraction at pH 5.85 and one minor fraction at pH 7.97, whereas only the pH 7.97 band was observed in the adult. It is concluded that the physical properties of the haemoglobin molecule itself are the same in newborn and adult rabbits, and that the postnatal decrease in haemoglobin O2 affinity is solely due to changes in the erythrocyte metabolism, leading to a marked rise in 2,3-DPG and a minor decline in erythrocyte pH. The rise in 2,3-DPG is caused by decrease of pyruvate kinase, probably due to proteolytic inactivation of the enzyme.

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Erythrocyte 2,3-Diphosphogly cerate, Po250%, and Available Oxygen in Young Rabbits with and without Postnatal Fall in Hemoglobin

Cited by 10 publications

References 12 publications

Use of Erythropoietin and Its Effects on Blood Lactate and 2,3-Diphosphoglycerate in Premature Neonates

Use of Erythropoietin and Its Effects on Blood Lactate and 2,3-Diphosphoglycerate in Premature Neonates

Regulation of Erythropoiesis in Suckling Rabbits with and without Postnatal Anemia: Partial Suppression of Production/Release of Erythropoiesis Stimulating Factor(s) by Iron Supplements

Haemoglobin O₂ binding in newborn and adult rabbits

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Erythrocyte 2,3-Diphosphogly cerate, Po250%, and Available Oxygen in Young Rabbits with and without Postnatal Fall in Hemoglobin

Cited by 10 publications

References 12 publications

Use of Erythropoietin and Its Effects on Blood Lactate and 2,3-Diphosphoglycerate in Premature Neonates

Use of Erythropoietin and Its Effects on Blood Lactate and 2,3-Diphosphoglycerate in Premature Neonates

Regulation of Erythropoiesis in Suckling Rabbits with and without Postnatal Anemia: Partial Suppression of Production/Release of Erythropoiesis Stimulating Factor(s) by Iron Supplements

Haemoglobin O2 binding in newborn and adult rabbits

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Haemoglobin O₂ binding in newborn and adult rabbits