Erythrocyte anion exchanger activity and intracellular pH in essential hypertension.

Abstract

The present study was designed to examine the activity of the sodium-independent chloride-bicarbonate anion exchanger and the sodium-proton exchanger in erythrocytes of 30 normotensive and 35 hypertensive subjects and its relation to the previously reported decrease in erythrocyte pH. Erythrocyte cytosolic pH was measured by the pH-sensitive fluorescent probe 2'-7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein. The activity of the anion exchanger was determined by acidifying cell pH and measuring the initial rate of the net sodium-independent, 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid-sensitive, bicarbonate influx driven by an outward proton gradient. The activity of the sodium-proton exchanger was determined by acidifying cell pH and measuring the initial rate of the net sodium-dependent proton efflux driven by an outward proton gradient. The activity of the anion exchanger was higher in hypertensive than control individuals (

Influence of hypertension on pH of saliva in older adults

“…The cause of decreasing salivary pH might be a change in the general status secondary to hypertension. The blood pressure influences the general condition in several ways (Alonso et al , ; Wong, ), which our results helped to demonstrate.…”

Abnormal Na+/H+ antiporter phenotype and turnover of immortalized lymphoblasts from type 1 diabetic patients with nephropathy.

“…In the presence of 24 mM NaHCO3 (pH 7.4, with 5% C02), there was no significant difference between the resting pHi of lymphoblasts from the DN and DCON patients and CON subjects. This could have been because of enhanced buffering capacity in the presence ofHCO3 and/or enhanced activity of anion exchangers as described recently by Alonso et al (30) in hypertensive patients.…”

Utilizing hydrogen sulfide as a novel anti‐cancer agent by targeting cancer glycolysis and pH imbalance

“…Resting pH i of cells was obtained in mammalian Ringer's solution with real‐time monitoring mode. Cells were then challenged with either alkali (20 mM HEPES, 20 mM NH 4 Cl, 5 mM KCl, 2 mM CaCl 2 , 1 mM MgCl 2 , 10 mM glucose; Alonso et al ., ) or acid [20 mM HEPES, 5 mM KCl, 2 mM CaCl 2 , 1 mM MgCl 2 , 10 mM glucose (pH 6.0); Furtado, ] loading buffer. Changes in pH i following challenges correspond to the activity of AEs and were monitored continuously.…”