Erythrocyte-enabled immunomodulation for vaccine delivery

Wang, Fei; Zong, Rongling; Chen, Gang

doi:10.1016/j.jconrel.2021.11.035

Cited by 19 publications

(7 citation statements)

References 124 publications

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“…The irons released from cellfree hemoglobin also contributed to bacterial growth and in ammation [50,51]. In addition, erythrocytes are involved in immune regulation by controlling the function of T lymphocytes, macrophages and DCs through surface molecules, and they can also adsorb and kill bacteria in circulation before presenting them to APCs for phagocytosis [52]. Therefore, in sepsis patients, ADRB2 may be regulated by hsa-miR-34b-3p and subsequently affects SPTB function, leading to abnormal erythrocytes in vivo, releasing intracellular contents, and preventing erythrocyte immune function, ultimately worsening sepsis.…”

Section: Discussionmentioning

confidence: 99%

Hsa-miR-34b-3p alleviates sepsis by relieving autoimmunosuppressive effects of ADRB2

Chen

Zheng

et al. 2023

Preprint

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Objective In this study, we aimed to identify the key microRNAs (miRNAs) and potential target genes through bioinformatics analysis, and investigate the underlying mechanisms of sepsis.Materials and Methods We collected miRNA expression profiles from sepsis patients and healthy individuals, screened differentially expressed miRNAs (DEMs) between sepsis patients and healthy individuals by bioinformatics analysis, and constructed miRNA-mRNA regulatory networks using online databases. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was used to annotate the biological functions and pathways of the genes. Single Sample Gene Set Enrichment Analysis (ssGSEA) assessed immunological characteristics in sepsis samples. Single cell sequencing (scRNA-seq) data were used to discover gene expression in different cell clusters.Results Four miRNAs were significantly differentially expressed in sepsis patients compared to healthy controls, with hsa-miR-34b-3p, hsa-miR-3663-3p and hsa-miR-4446-5p upregulated and hsa-miR-625-5p downregulated. ADRB2 may be a potential target of hsa-miR-34b-3p, and DisGeNET database showed that ADRB2 may be related to sepsis. Receiver operating characteristic (ROC) analysis suggested that ADRB2 has potential as a diagnostic marker for sepsis. The ssGSEA result showed that ADRB2 expression was positively correlated with T cell co-inhibition, and negatively correlated with dendritic cell infiltration. ScRNA-seq data showed that ADRB2 expression was increased in natural killer (NK) cells and natural killer T (NKT) cells in sepsis patients in contrast to healthy controls.Conclusion ADRB2 may suppress the autoimmunity of patients with sepsis, thus aggravating sepsis. It can be used as a new diagnostic biomarker and molecular therapeutic target. Hsa-miR-34b-3p can inhibit the expression of ADRB2, relieve its immunosuppressive effect and alleviate sepsis to a certain extent.

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Section: Discussionmentioning

confidence: 99%

Hsa-miR-34b-3p alleviates sepsis by relieving autoimmunosuppressive effects of ADRB2

Chen

Zheng

et al. 2023

Preprint

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“…This approach has great potential for the treatment of a number of diseases 98 . Nanocarriers that hitch a ride on RBCs in a non-covalent manner can travel further and more efficiently throughout the body, which can improve their therapeutic effects 99 . This is because RBCs are naturally transported to specific organs, such as the lungs and liver.…”

Section: Red Cell-based Advanced Drug Delivery Systemmentioning

confidence: 99%

“…This is because these cells are not as effective at presenting antigens to APCs as healthy erythrocytes. This makes it crucial to control the type of erythrocytes used in EDIT for tumor immunotherapy 99 .…”

Section: Rbc-inspired Cancer Immunotherapymentioning

confidence: 99%

Red blood cells in biology and translational medicine: natural vehicle inspires new biomedical applications

Zhang,

Lin,

Xin

et al. 2024

Theranostics

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Red blood cells (RBCs) are the most abundant cell type in the blood, and play a critical role in oxygen transport. With the development of nanobiotechnology and synthetic biology, scientists have found multiple ways to take advantage of the characteristics of RBCs, such as their long circulation time, to construct universal RBCs, develop drug delivery systems, and transform cell therapies for cancer and other diseases. This article reviews the component and aging mystery of RBCs, the methods for the applied universal RBCs, and the application prospects of RBCs, such as the engineering modification of RBCs used in cytopharmaceuticals for drug delivery and immunotherapy. Finally, we summarize some perspectives on the biological features of RBCs and provide further insights into translational medicine.

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“…[4][5] Over the last decade, the importance of the role of this organ in response to ischemic myocardial damage has been investigated. [4,[6][7][8][9] Upon infarction, increased angiotensin II levels promote the migration of monocytes from the spleen to the heart where they differentiate into macrophages and partake in the inflammatory phase of the insult. [10] Days after the initial injury, the inflammatory milieu changes to one that is reparative, suppressing inflammation and inducing matrix deposition and angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

Biomimetic Nanomaterials for the Immunomodulation of the Cardiosplenic Axis Postmyocardial Infarction

Bose,

Kessinger,

Dhammu

et al. 2023

Advanced Materials

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The spleen is an important mediator of both adaptive and innate immunity. As such, attempts to modulate the immune response provided by the spleen may be conducive to improved outcomes for numerous diseases throughout the body. Here, biomimicry is used to rationally design nanomaterials capable of splenic retention and immunomodulation for the treatment of disease in a distant organ, the postinfarct heart. Engineered senescent erythrocyte‐derived nanotheranostic (eSENTs) are generated, demonstrating significant uptake by the immune cells of the spleen including T and B cells, as well as monocytes and macrophages. When loaded with suberoylanilide hydroxamic acid (SAHA), the nanoagents exhibit a potent therapeutic effect, reducing infarct size by 14% at 72 h postmyocardial infarction when given as a single intravenous dose 2 h after injury. These results are supportive of the hypothesis that RBC‐derived biomimicry may provide new approaches for the targeted modulation of the pathological processes involved in myocardial infarction, thus further experiments to decisively confirm the mechanisms of action are currently underway. This novel concept may have far‐reaching applicability for the treatment of a number of both acute and chronic conditions where the immune responses are either stimulated or suppressed by the splenic (auto)immune milieu.

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Erythrocyte-enabled immunomodulation for vaccine delivery

Cited by 19 publications

References 124 publications

Hsa-miR-34b-3p alleviates sepsis by relieving autoimmunosuppressive effects of ADRB2

Hsa-miR-34b-3p alleviates sepsis by relieving autoimmunosuppressive effects of ADRB2

Red blood cells in biology and translational medicine: natural vehicle inspires new biomedical applications

Biomimetic Nanomaterials for the Immunomodulation of the Cardiosplenic Axis Postmyocardial Infarction

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