Erythrocyte Glutathione Synthesis in the Neonate

Minnich, Virginia; Smith, Mary E.; Rajanasathit, Chinda; Majerus, Philip W.

doi:10.1159/000240642

Cited by 8 publications

(5 citation statements)

References 7 publications

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“…Konrad et al [33] found higher GSH concentrations in cord erythrocytes than in normal nonneonatal blood and in nonneonatal blood with comparable reticulocytosis. Minnich et al [35] found higher GSH concentrations in cord blood of neonates than in maternal blood. The small but significant increase (+13%) in GSH levels found by us in term newborns with respect to adults confirms results of other studies reported in the literature [34,35].…”

Section: Discussionmentioning

confidence: 96%

“…Minnich et al [35] found higher GSH concentrations in cord blood of neonates than in maternal blood. The small but significant increase (+13%) in GSH levels found by us in term newborns with respect to adults confirms results of other studies reported in the literature [34,35]. Moreover, our data are the first ever to indicate simultaneously higher GSH and GSSG levels and antioxidant enzyme activities in RBC of premature newborns than in those of adults.…”

Section: Discussionmentioning

confidence: 96%

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Glutathione Recycling and Antioxidant Enzyme Activities in Erythrocytes of Term and Preterm Newborns at Birth

Frosali¹,

Simplicio²,

Perrone

et al. 2004

Neonatology

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We previously demonstrated a high susceptibility of neonatal red blood cells (RBC) to oxidative stress at birth. The aim of this study was to compare the RBC antioxidant capacity and redox cycle enzyme activities as well as glutathione (GSH) recycling in full-term and preterm infants at birth and in normal adults. GSH and GSH disulfide (GSSG) concentrations, GSH/GSSG ratio, and the activities of glucose-6-phosphate dehydrogenase (G-6-PDH), GSH peroxidase, GSH reductase (GR), catalase (CAT), superoxide dismutase (SOD), and hexokinase (HK) were measured in RBC of 25 healthy adults and 56 newborns (23 term, 33 preterm) at birth. The GSH recycling was measured in adult and newborn RBC exposed to oxidative stress (1 mM tert-butylhydroperoxide). The RBC of term and preterm babies showed higher GSH, GSSG, G-6-PDH, GR, and HK levels/activities and lower GSH/GSSG ratios and higher GSH-recycling rates than those of adults. In preterm babies significant correlations were found between G-6-PDH and CAT, GSH, GSH/GSSG ratio, and GSSG (r = –0.67, r = 0.71, r = –0.66, p < 0.01; r = 0.71, p < 0.05, respectively). In term newborns, statistically significant correlations were observed between G-6-PDH and CAT, SOD, and GSH (r = –0.65, r = –0.65, r = –0.69, p < 0.01, respectively). The results indicate the central role of the G-6-PDH activity in antioxidant defenses. We speculate that preterm babies have prompter involvement of antioxidant defenses than term babies.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 96%

Glutathione Recycling and Antioxidant Enzyme Activities in Erythrocytes of Term and Preterm Newborns at Birth

Frosali¹,

Simplicio²,

Perrone

et al. 2004

Neonatology

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“…Red cells of newborn infants possess a higher level of adenosine triphosphate (ATP) (14,15), higher activity of many glycolytic enzymes and consume glucose at rates considerably faster than those observed in the cells of the normal adult (12,15,16). In spite of similar levels of 2,3-diphosphoglycerate (2,3-DPG) in the red blood cells, there are considerable differences between fetal and adult haemoglobins for oxygen affinity (13).…”

Section: Introductionmentioning

confidence: 99%

<i>In vitro</i> Metabolism of Red Blood Cells from Newborn and Adult Goats

Agar¹,

Harley²

1976

Neonatology

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The erythrocytes of newborn and adult goats were studied with respect to GSH stability, GSH regeneration, levels of ATP and 2,3-DPG, glucose consumption and activities of nine different enzymes of Embden-Meyerhof and pentose phosphate pathways of glucose metabolism. Red cells from newborn goats had significantly higher levels of ATP and 2,3-DPG. The activities of all the enzymes measured were also significantly greater in the newborn. It is suggested that newborn goats possess red blood cells that are metabolically more active than those of adult goats.

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“…26 Minnich et al found higher GSH concentrations in cord blood of neonates than in maternal blood. 28 Frosali et al reported simultaneously higher GSH and GSSG levels and antioxidant enzyme activities in RBC of premature newborns than in those of adults. 20 All these results clearly show the great antioxidant resources of these cells against stressors associated with prematurity.…”

Section: Glutathione Recycling In Term and Preterm Newbornsmentioning

confidence: 97%

Enzyme Activities in Erythrocytes of Term and Preterm Newborns

et al. 2016

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The decrease of erythrocyte antioxidant enzyme activity should be considered as a possible cause of hemolysis when there is no evidence of an immune-mediated hemolytic anemia, no consumptive red blood cell disorder, no morphologic or laboratory data to suggest a problem of the red cell membrane, and no evidence of a quantitative or qualitative defect in hemoglobin synthesis. Glutatione has a pilot role in orchestrating the redox balance when there is an excess reactive oxygen species production or defective antioxidant defenses that may have deleterious consequences in various perinatal diseases. Neonatal erythrocytes are a target of extracellular free radicals and in the same time generators of free radicals through Fenton reaction. The complex mechanism underlying the increased oxidative stress susceptibility of neonatal erythrocytes requires further investigation.

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Erythrocyte Glutathione Synthesis in the Neonate

Cited by 8 publications

References 7 publications

Glutathione Recycling and Antioxidant Enzyme Activities in Erythrocytes of Term and Preterm Newborns at Birth

Glutathione Recycling and Antioxidant Enzyme Activities in Erythrocytes of Term and Preterm Newborns at Birth

<i>In vitro</i> Metabolism of Red Blood Cells from Newborn and Adult Goats

Enzyme Activities in Erythrocytes of Term and Preterm Newborns

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