Erythrocyte lysis and angle‐resolved light scattering measured by scanning flow cytometry result to 48 indices quantifying a gas exchange function of the human organism

Abstract

Molecular/cell level of gas exchange function assumes the accurate measurement of erythrocyte characteristics and rate constants concerning to molecules involved into the CO 2 /O 2 transport. Unfortunately, common hematology analyzers provide the measurement of eight indices of erythrocytes only and say little about erythrocyte morphology and nothing about rate constants of cellular function. The aim of this study is to demonstrate the ability of the Scanning Flow Cytometer (SFC) in the complete morphological analysis of mature erythrocytes and characterization of erythrocyte function via measurement of lysing kinetics. With this study we are introducing 48 erythrocyte indices. To provide the usability of application of the SFC in clinical diagnosis, we formed four categories of indices which are as follows: content/ concentration (9 indices), morphology (26 indices), age (5 indices), and function (8 indices). The erythrocytes of 39 healthy volunteers were analyzed with the SFC to fix the first-ever reference intervals for the new indices introduced. The essential measurable reliability of the presented method is expressed in terms of errors of characteristics of single erythrocytes retrieved from the solution of the inverse lightscattering problem and errors of parameters retrieved from the fitting of the experimental kinetics by molecular-kinetics model of erythrocyte lysis.

A light scatter based model relating erythrocyte vesiculation to lifetime in circulation

“…Finally, the algorithm allows us to construct the distribution and to calculate the mean and standard deviation of erythrocyte derived age. The symmetry of distribution is hereinafter defined as a percent of cells, whose characteristics do not exceed the distribution mode, the value that appears most often in a set of data values [28]. Moreover, the mean daily acts of vesiculation M d and additional volume V add can be determined by the proposed algorithm from analysis of SI & S scatterplots of erythrocyte samples.…”

“…(Novosibirsk, Russia) and equipped with a 40 mW laser of 660 nm (LM-660-20-S) to illuminate individual cells for angle-resolved light scattering and 488-nm laser (15 mW, FCD488-020, JDS Uniphase Corporation, Milpitas, CA) to illuminate the cells for forward and side scattering signals in a conventional manner. Detailed technical features of the SFC and the operational function of the optical cell were previously described elsewhere [28,33,38]. The operational angular range θ of the SFC was determined from the analysis of polystyrene microspheres to be from 10 to 70 [39].…”

“…Finally, the algorithm allows us to construct the distribution and to calculate the mean and standard deviation of erythrocyte derived age. The symmetry of distribution is hereinafter defined as a percent of cells, whose characteristics do not exceed the distribution mode, the value that appears most often in a set of data values [28].…”

“…In our previous study [28] the derived age of an erythrocyte was evaluated using the phenomenon that erythrocytes lose a part of their volume and hemoglobin during their 115-day lifespan [29] in the bloodstream. Have to note that the derived age does not exactly mean the real age of erythrocytes and its evaluation utilizes changes of cellular morphology during a lifespan.…”

The effect of silver-containing sorbent on red blood cells during hemosorption: an <i>in vitro</i> study

“…Как видно из табл. 1, наблюдается статистически незначимое увеличение площади и объема клеток под влиянием сорбентов относительно исходных показателей, однако все морфологические параметры остаются в пределах нормы [11,12], т.е. сорбенты не оказывают повреждающего токсического воздействия.…”