Erythrocyte‐magnetized technology: an original and innovative method for blood group serology

Bouix, O.; Ferrera, Virginie; Delamaire, M.; Redersdorff, Jean Claude; Roubinet, F.

doi:10.1111/j.1537-2995.2008.01790.x

Cited by 19 publications

(27 citation statements)

References 13 publications

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“…Discrepancies were usually a result of weak reactions in manual testing; both studies indicated that this device is more sensitive in detecting agglutination reactions. The QWALYS system utilizes erythrocyte-magnetized technology [25], and its performance in ABO blood typing has been reviewed by many researchers. The QWALYS-2 device was examined by Schoenfeld et al [26], who concluded that it was suitable for ABO grouping.…”

Section: Discussionmentioning

confidence: 99%

ABO Mistyping of cis-AB Blood Group by the Automated Microplate Technique

Chun

Ryu

Cha

et al. 2018

Transfus Med Hemother

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Background: The cis-AB phenotype, although rare, is the relatively most frequent of ABO subgroups in Koreans. To prevent ABO mistyping of cis-AB samples, our hospital has applied a combination of the manual tile method with automated devices. Herein, we report cases of ABO mistyping detected by the combination testing system. Methods: Cases that showed discrepant results by automated devices and the manual tile method were evaluated. These samples were also tested by the standard tube method. The automated devices used in this study were a QWALYS-3 and Galileo NEO. Exons 6 and 7 of the ABO gene were sequenced. Results: 13 cases that had the cis-AB allele showed results suggestive of the cis-AB subgroup by manual methods, but were interpreted as AB by either automated device. This happened in 87.5% of these cases by QWALYS-3 and 70.0% by Galileo NEO. Genotyping results showed that 12 cases were ABO*cis-AB01/ABO*O01 or ABO*cis-AB01/ABO*O02, and one case was ABO*cis-AB01/ ABO*A102. Conclusion: Cis-AB samples were mistyped as AB by the automated microplate technique in some cases. We suggest that the manual tile method can be a simple supplemental test for the detection of the cis-AB phenotype, especially in countries with relatively high cis-AB prevalence.

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Section: Discussionmentioning

confidence: 99%

ABO Mistyping of cis-AB Blood Group by the Automated Microplate Technique

Chun

Ryu

Cha

et al. 2018

Transfus Med Hemother

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“…[12] The introduction of newer techniques such as column agglutination technique (CAT), Solid Phase Red Cell Adherence Assay (SPRCA), and erythrocyte-magnetized technique (EMT) have tried to overcome these short comings and bring about an improvement in the quality of testing and the reproducibility of results. [1–3] These new technologies are amenable to automation and major manufacturers in this field have come up with user-friendly semi-automated and fully automated equipments for immunohematology tests with varying throughputs depending upon the work load of a blood bank. We, here, present a brief introduction of major equipments available and the requirements for shifting from the conventional technology to the automated systems.…”

Section: Introductionmentioning

confidence: 99%

“…In the available studies, it has been shown a performance comparable with that of gel CAT. [3] A comparison of the newer technologies with the conventional tube testing has been compiled in Table 1. [178101415] The newer methods have added to the quality of testing but none of the methods has been found to be unequivocally superior to others.…”

Section: Introductionmentioning

confidence: 99%

Automation in Immunohematology

Bajpai

Kaur

Gupta

2012

Asian J Transfus Sci

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There have been rapid technological advances in blood banking in South Asian region over the past decade with an increasing emphasis on quality and safety of blood products. The conventional test tube technique has given way to newer techniques such as column agglutination technique, solid phase red cell adherence assay, and erythrocyte-magnetized technique. These new technologies are adaptable to automation and major manufacturers in this field have come up with semi and fully automated equipments for immunohematology tests in the blood bank. Automation improves the objectivity and reproducibility of tests. It reduces human errors in patient identification and transcription errors. Documentation and traceability of tests, reagents and processes and archiving of results is another major advantage of automation. Shifting from manual methods to automation is a major undertaking for any transfusion service to provide quality patient care with lesser turnaround time for their ever increasing workload. This article discusses the various issues involved in the process.

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“…The principles of EMT were described previously 1,2 . The EMT is based on the adsorption of paramagnetic compounds on the membrane of the RBCs.…”

Section: Methodsmentioning

confidence: 99%

“…The principle of EMT is based on magnetization of red blood cells (RBCs) and avoids centrifugation and washing steps. This new method was recently described in detail 2 …”

mentioning

confidence: 99%