Erythrocyte membrane-camouflaged carrier-free nanoassembly of FRET photosensitizer pairs with high therapeutic efficiency and high security for programmed cancer synergistic phototherapy

Zhang, Xuanbo; Xiong, Jianchen; Wang, Kaiyuan; Ye, Han; Sun, Bingjun; Hao, Yue; Zhao, Zhiqiang; Wang, Ning; Wang, Yuequan; Zhang, Shenwu; Zhao, Wenxuan; Zhang, Haotian; Zhang, He; Luo, Cong; Sun, Jin

doi:10.1016/j.bioactmat.2021.01.004

Cited by 48 publications

(30 citation statements)

References 35 publications

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“…According to a previous report [ 42 , 43 ], 1 × 10 7 C6 cells were intracerebrally injected into the brain of ICR mice. The mice were divided into four groups and injected with 100 μL of normal saline, DiR, DiR-CCM-(PTX)NS, or DiR- D WSW-CCM-(PTX)NS [ 44 ]. At 2, 4, 8, 12, 24, and 36 h after administration, the mice were anesthetized with 3% isoflurane and analyzed (748/780 nm) using an IVIS in vivo system (IVIS® Spectrum, PerkinElmer, USA).…”

Section: Methodsmentioning

confidence: 99%

Carrier-free highly drug-loaded biomimetic nanosuspensions encapsulated by cancer cell membrane based on homology and active targeting for the treatment of glioma

Fan

Cui²,

Hao³

et al. 2021

Bioactive Materials

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Nanosuspensions, as a new drug delivery system for insoluble drugs, are only composed of a drug and a small amount of stabilizer, which is dispersed in an aqueous solution with high drug-loading, small particle size, high dispersion, and large specific surface area. It can significantly improve the dissolution, bioavailability, and efficacy of insoluble drugs. In this study, paclitaxel nanosuspensions ((PTX)NS) were prepared by an ultrasonic precipitation method, with the characteristics of simple preparation and easy repetition. With the help of a homologous targeting mechanism, a kind of glioma C6 cancer cell membrane (CCM)-coated (PTX)NS was developed and modified with D WSW peptide to obtain D WSW-CCM-(PTX)NS with the functions of BBB penetration and tumor targeting. The results showed that the cancer cell membrane could effectively camouflage the nanosuspensions so that it was not cleared by the immune system and could cross the blood-brain-barrier (BBB) and selectively target tumor tissues. Cell uptake experiments and in vivo imaging confirmed that the uptake of D WSW-CCM-(PTX)NS by tumor cells and the distribution in intracranial gliomas increased. Cytotoxicity test and in vivo anti-glioma studies showed that D WSW-CCM-(PTX)NS could significantly inhibit the growth of glioma cells and significantly prolong the survival time of glioma-bearing mice. Finally, the cancer cell membrane coating endowed the nanosuspensions with the biological properties of homologous adhesion and immune escape. This study provides an integrated solution for improving the targeting of nanosuspensions and demonstrates the encouraging potential of biomimetic nanosuspensions applicable to tumor therapy.

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Section: Methodsmentioning

confidence: 99%

Carrier-free highly drug-loaded biomimetic nanosuspensions encapsulated by cancer cell membrane based on homology and active targeting for the treatment of glioma

Fan

Cui²,

Hao³

et al. 2021

Bioactive Materials

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“…As for hydrophobic molecules, due to the high surface free energy, a small amount of surfactants may be added to improve the colloidal stability 22 , 25 . The developed PDNAs for cancer therapy are summarized in Table 1 22 , 23 , 25 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 . In this section, the three types of PDNAs will be introduced and we will take an insight into the assembly mechanisms of PDNAs.…”

Section: Nano-assembly Of Pure Drug Moleculesmentioning

confidence: 99%

“…Recently, pure drug nano-assemblies (PDNAs), fabricated by the self-assembly or co-assembly of pure drug molecules, have attracted considerable attention. Drug molecules without any chemical modification could spontaneously form uniform nanoparticles (NPs), usually by the one-step nanoprecipitation method 22 , 23 . This is different from the traditional nanocrystal preparations, obtained by pearl milling, high pressure homogenization, etc.…”

Section: Introductionmentioning

confidence: 99%

Pure drug nano-assemblies: A facile carrier-free nanoplatform for efficient cancer therapy

Zang

et al. 2022

Acta Pharmaceutica Sinica B

124

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Nanoparticulate drug delivery systems (Nano-DDSs) have emerged as possible solution to the obstacles of anticancer drug delivery. However, the clinical outcomes and translation are restricted by several drawbacks, such as low drug loading, premature drug leakage and carrier-related toxicity. Recently, pure drug nano-assemblies (PDNAs), fabricated by the self-assembly or co-assembly of pure drug molecules, have attracted considerable attention. Their facile and reproducible preparation technique helps to remove the bottleneck of nanomedicines including quality control, scale-up production and clinical translation. Acting as both carriers and cargos, the carrier-free PDNAs have an ultra-high or even 100% drug loading. In addition, combination therapies based on PDNAs could possibly address the most intractable problems in cancer treatment, such as tumor metastasis and drug resistance. In the present review, the latest development of PDNAs for cancer treatment is overviewed. First, PDNAs are classified according to the composition of drug molecules, and the assembly mechanisms are discussed. Furthermore, the co-delivery of PDNAs for combination therapies is summarized, with special focus on the improvement of therapeutic outcomes. Finally, future prospects and challenges of PDNAs for efficient cancer therapy are spotlighted.

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“…Photothermal therapy (PTT) has attracted considerable attention as a promising non-invasive therapeutic modality with spatio-temporal selectivity and high security [ 6 – 9 ]. Series of organic photothermal agents have been found to exert antitumor activity by converting the near-infrared (NIR) light into tumor-localized heat [ 10 , 11 ]. Moreover, fluorescence characteristics in most photosensitizers (PSs) endows them a natural advantage in real-time tumor imaging and therapeutic monitoring [ 12 – 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…Despite of all these advantages, PTT alone is usually unable to completely eradicate tumors, including 4T1 breast tumors [ 11 , 15 , 19 – 22 ]. One of the major reasons should be the cytoprotective pathways towards hyperthermia activated in tumor cells under laser irradiation, which inducing the overexpression of heat shock proteins (HSPs) under thermal stimulation [ 19 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Molecularly engineered carrier-free co-delivery nanoassembly for self-sensitized photothermal cancer therapy

et al. 2021

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Background Photothermal therapy (PTT) has been extensively investigated as a tumor-localizing therapeutic modality for neoplastic disorders. However, the hyperthermia effect of PTT is greatly restricted by the thermoresistance of tumor cells. Particularly, the compensatory expression of heat shock protein 90 (HSP90) has been found to significantly accelerate the thermal tolerance of tumor cells. Thus, a combination of HSP90 inhibitor and photothermal photosensitizer is expected to significantly enhance antitumor efficacy of PTT through hyperthermia sensitization. However, it remains challenging to precisely co-deliver two or more drugs into tumors. Methods A carrier-free co-delivery nanoassembly of gambogic acid (GA, a HSP90 inhibitor) and DiR is ingeniously fabricated based on a facile and precise molecular co-assembly technique. The assembly mechanisms, photothermal conversion efficiency, laser-triggered drug release, cellular uptake, synergistic cytotoxicity of the nanoassembly are investigated in vitro. Furthermore, the pharmacokinetics, biodistribution and self-enhanced PTT efficacy were explored in vivo. Results The nanoassembly presents multiple advantages throughout the whole drug delivery process, including carrier-free fabrication with good reproducibility, high drug co-loading efficiency with convenient dose adjustment, synchronous co-delivery of DiR and GA with long systemic circulation, as well as self-tracing tumor accumulation with efficient photothermal conversion. As expected, HSP90 inhibition-augmented PTT is observed in a 4T1 tumor BALB/c mice xenograft model. Conclusion Our study provides a novel and facile dual-drug co-assembly strategy for self-sensitized cancer therapy. Graphic abstract

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Erythrocyte membrane-camouflaged carrier-free nanoassembly of FRET photosensitizer pairs with high therapeutic efficiency and high security for programmed cancer synergistic phototherapy

Cited by 48 publications

References 35 publications

Carrier-free highly drug-loaded biomimetic nanosuspensions encapsulated by cancer cell membrane based on homology and active targeting for the treatment of glioma

Carrier-free highly drug-loaded biomimetic nanosuspensions encapsulated by cancer cell membrane based on homology and active targeting for the treatment of glioma

Pure drug nano-assemblies: A facile carrier-free nanoplatform for efficient cancer therapy

Molecularly engineered carrier-free co-delivery nanoassembly for self-sensitized photothermal cancer therapy

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