Erythrocyte osmotic fragility and lipid peroxidation following chronic co-exposure of rats to chlorpyrifos and deltamethrin, and the beneficial effect of alpha-lipoic acid

Uchendu, Chidiebere; Ambali, Suleiman Folorunsho; Ayo, Joseph Olusegun; Esievo, K. A. N.; Umosen, Angela J.

doi:10.1016/j.toxrep.2014.07.002

Cited by 29 publications

(18 citation statements)

References 29 publications

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“…Human beings and animals are often exposed to mixtures of environmental contaminants including pesticides and metals [[31], [32], [33]]. Earlier investigations demonstrated that exposure to pesticides including fipronil, chlorpyrifos and deltamethrin disrupted antioxidant defense system which consequently induced oxidative stress in experimental animals whereas the adverse effects were ameliorated by the administration of exogenous antioxidants [32,34]. However, continuous low-dose dietary exposure to pesticide α-cypermethrin reportedly did not induce liver damage, oxidative stress and impaired antioxidant defense in rats [35].…”

Section: Discussionmentioning

confidence: 99%

Manganese suppresses oxidative stress, inflammation and caspase-3 activation in rats exposed to chlorpyrifos

Owumi

Dim

2019

Toxicology Reports

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The present study investigated the individual and combined impact of organophosphorus pesticide chlorpyrifos (CPF) and manganese (Mn), a naturally occurring trace metal, on hepatorenal function in adult rats. The four experimental groups namely control, CPF alone (5 mg/kg), Mn alone (10 mg/kg) and the co-exposure group consisted of eight rats which were orally gavage for 14 consecutive days. Following sacrifice, the biomarkers of hepatorenal damage, antioxidant enzyme activities, myeloperoxidase (MPO) activity as well as levels of nitric oxide, reactive oxygen and nitrogen (RONS) species and lipid peroxidation (LPO) were analysed spectrophotometrically. Further, the concentration of tumour necrosis factor alpha (TNF-α), interleukin-1 β (IL-1β) and caspase-3 activity were assessed using ELISA. Results showed that the CPF-induced increase in biomarkers of hepatorenal toxicity were significantly (p < 0.05) alleviated in rats co-expose to CPF and Mn. Moreover, the decrease in antioxidant status as well as the elevation in RONS and LPO were significantly assuaged in rats co-treated with CPF and Mn. In addition, CPF mediated increase in TNF-α, IL-1β and caspase-3 activity were significantly diminished in the liver and kidney of rats co-exposed to CPF and Mn. Light microscopic examination evidenced that the severity of histopathological lesions induced by CPF were alleviated in rats co-exposed to CPF and Mn. In conclusion, the results highlight that co-exposure to CPF and Mn in rats assuaged CPF-induced oxidative stress, inflammation and caspase-3 activation in the liver and kidney of the rats.

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Section: Discussionmentioning

confidence: 99%

Manganese suppresses oxidative stress, inflammation and caspase-3 activation in rats exposed to chlorpyrifos

Owumi

Dim

2019

Toxicology Reports

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“…It also interfered with glucose-6-phosphate dehydrogenase activity that are required for membrane integrity (Mayes, 1983;Champe et al, 2005;Ojo et al, 2006). Although erythrocytes are well equipped with several biological mechanisms to defend against intracellular oxidative stress (Prasanthi et al, 2005) they can be oxidatively damaged due to toxic chemicals and environmental pollutants (Uchendu et al, 2014). Another view of the mechanism of fragility is that the hydrocarbon component also interfered with the membrane sodium pump mechanism that maintains a low level of sodium ion concentration inside the cell.…”

Section: Discussionmentioning

confidence: 99%

The ameliorative effects of <i>Moringa oleifera</i> leaf extract on cardiovascular functions and osmotic fragility of wistar rats exposed to petrol vapour

Azeez¹,

Adah²,

Olaifa³

et al. 2017

Sokoto J. Vet. Sc.

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The ameliorative effects of Moringa oleifera leaf extract on cardiovascular functions and osmotic fragility of wistar rats exposed to petrol vapour OM Azeez*, SA Adah, FH Olaifa, A Basiru & R AbdulbakiUniversity of Ilorin, Ilorin, Nigeria *Correspondence: Tel.: +2348033858362; E-mail: misibisol@yahoo.com Abstract The present study was aimed at evaluating the ameliorating effects of Moringa oleifera extract compared to captopril and candesartan cilexetil on cardiovascular functions and osmotic fragility of rats exposed to petrol vapour. Twenty five adult male Wistar rats (130g-200g) were randomly grouped to five with five rats in a group. Group 1 (control) was not exposed to petrol fume. Groups 2 (petrol only), was exposed to petrol fume only. Groups 3, 4 and 5 were pretreated with Moringa oleifera extract (40mg/kg), captopril (25 mg/kg) and candesartan (16mg/kg), respectively before exposure to petrol vapour, 10 minutes every day for eight weeks. All groups were given feed and water ad-libitum. Petrol vapour was generated by using human compressor nebulizer adopted for rats and connected to fume chamber where the rats were kept. The pretreatment were administered by oral cannula. At the end of the exposure, 0.2ml of blood samples obtained from individual rat in each group were suspended in separate sets of Phosphate buffer saline (PBS) solution of decreasing concentrations. Erythrocyte osmotic fragility (EOF) was determined by spectrophotometer. Electrocardiography was done using EDAN 10. There was significant increase (p<0.05) in EOF of the rats exposed to petrol vapour only. However, Moringa oleifera, captopril and candesartan cilexetil significantly ameliorated this effect. There was no significant difference in the amelioration of Moringa oleifera and candesartan cilexetil. There was absence of p-wave and significant increase in heart rate observed in the electrocardiogram of petrol only group, this was significantly restored in the Moringa oleifera, captopril and the candesartan cilexetil group. The results showed that exposure to petrol vapour elevated EOF, resulted in atria arrhythmia and increased heart rate. These effects were ameliorated by pretreatment with Moringa oleifera, captopril and candesartan cilexetil. The amelioration in Moringa oleifera was comparable with that of candesartan cilexetil suggesting that Moringa oleifera may be an Angiotensin II receptor blocker.

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“…Several in vitro [21,22] and in vivo [23,24] studies have demonstrated that chlorpyrifos exposure induces oxidative stress. Oxidative stress is known to activate redox sensitive kinases (RSKs) like NFκB (Nuclear Factor kappa B) and p38 MAPK (p38 Mitogen-Activated Protein Kinase) [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Effect of sub-toxic chlorpyrifos on redox sensitive kinases and insulin signaling in rat L6 myotubes

Shrestha

Singh

Sarkar

et al. 2018

J Diabetes Metab Disord

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Objectives Sub-chronic exposures to chlorpyrifos, an organophosphorus pesticide is associated with incidence of diabetes mellitus. Biochemical basis of chlorpyrifos-induced diabetes mellitus is not known. Hence, effect of its sub-toxic exposure on redox sensitive kinases, insulin signaling and insulin-induced glucose uptake were assessed in rat muscle cell line. Methods In an in vitro study, rat myoblasts (L6) cell line were differentiated to myotubes and then were exposed to subtoxic concentrations (6 mg/L and 12 mg/L) of chlorpyrifos for 18 h. Then total anti-oxidant level in myotubes was measured and insulin-stimulated glucose uptake was assayed. Assessment of activation of NFκB & p38MAPK and insulin signaling following insulin stimulation from tyrosine phosphorylation of insulin receptor substrate-1 (IRS-1) and serine phosphorylation of Akt were done in myotubes after chlorpyrifos exposure by western blot (WB) and compared with those in vehicle-treated controls. Results The glucose uptake and total antioxidant level in L6-derived myotubes after sub-toxic exposure to chlorpyrifos were decreased in a dose-dependent manner. As measured from band density of WB, phosphorylation levels increased for redosensitive kinases (p38MAPK and IκBα component of NFκB) and decreased for IRS-1 (at tyrosine 1222) and Akt (at serine 473) on insulin stimulation following chlorpyrifos exposure as compared to those in controls. Conclusion We conclude that sub-toxic chlorpyrifos exposure induces oxidative stress in muscle cells activating redox sensitive kinases that impairs insulin signaling and thereby insulin-stimulated glucose uptake in muscle cells. This probably explains the biochemical basis of chlorpyrifos-induced insulin resistance state and diabetes mellitus. Keywords Chlorpyrifos. Type 2 diabetes mellitus. Insulin signaling. Insulin resistance. NFκB. p38MAPK Key message 1. Sub-toxic chlorpyrifos exposure imparts oxidative stress and thus activates redox sensitive kinases. 2. The activated redox sensitive kinases impair insulin signaling, that in turn induce insulin resistance in muscle cells leading to decreased glucose uptake.

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Erythrocyte osmotic fragility and lipid peroxidation following chronic co-exposure of rats to chlorpyrifos and deltamethrin, and the beneficial effect of alpha-lipoic acid

Cited by 29 publications

References 29 publications

Manganese suppresses oxidative stress, inflammation and caspase-3 activation in rats exposed to chlorpyrifos

Manganese suppresses oxidative stress, inflammation and caspase-3 activation in rats exposed to chlorpyrifos

The ameliorative effects of <i>Moringa oleifera</i> leaf extract on cardiovascular functions and osmotic fragility of wistar rats exposed to petrol vapour

Effect of sub-toxic chlorpyrifos on redox sensitive kinases and insulin signaling in rat L6 myotubes

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