Erythromycin and Clindamycin Resistance and Telithromycin Susceptibility in
            <i>Streptococcus agalactiae</i>

Betriú, Carmen; Culebras, Esther; Gómez, Manuel; Rodríguez-Avial, Iciar; Sánchez, Blas Ali; Ágreda, M.; Picazo, Juan J.

doi:10.1128/aac.47.3.1112-1114.2003

Cited by 68 publications

(55 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This low prevalence of the mef(A) gene among our isolates agrees with that described in France, Spain and Canada (Fitoussi et al, 2001;Betriu et al, 2003;de Azavedo et al, 2001). An overall concordance was noted between the MLS phenotype and resistance genotype.…”

Section: Discussionsupporting

confidence: 78%

See 1 more Smart Citation

Macrolide and tetracycline resistance in clinical strains of Streptococcus agalactiae isolated in Tunisia

Hraoui¹,

Boubaker²,

Rachdi³

et al. 2012

Journal of Medical Microbiology

View full text Add to dashboard Cite

clinical strains of Streptococcus agalactiae, recovered from female genital specimens and from gastric fluid or ear specimens from infected newborns, were isolated at the Laboratory of Microbiology of Charles Nicolle Hospital of Tunis. They were investigated to determine the prevalence of antibiotic resistance and to characterize the mechanisms of resistance to macrolide and tetracycline. All strains were susceptible to penicillin, ampicillin and quinupristin-dalfopristin. They were resistant to chloramphenicol (3.1 %), rifampicin (19.1 %), erythromycin (40 %) and tetracycline (97.3 %); 3.1 % were highly resistant to streptomycin and 1.3 % to gentamicin. Among the erythromycin-resistant isolates, 78.7 % showed a constitutive macrolide-lincosamide-streptogramin B (MLS B ) phenotype with high-level resistance to macrolides and clindamycin (MIC 50 .256 mg ml ) and low MICs of clindamycin (MIC 50 58 mg ml "1 ) and 2.2 % showed an M phenotype with a low erythromycin-resistance level (MIC range512-32 mg ml "1 ) and low MICs of clindamycin (MIC range: 0.75-1 mg ml). All strains were susceptible to quinupristin-dalfopristin and linezolid (MIC 90 : 0.75 mg ml "1 for each). MLS B phenotypes were genotypically confirmed by the presence of the erm(B) gene and the M phenotype by the mef(A) gene. Resistance to tetracycline was mainly due to the tet(M) gene (93.1 %) encoding a ribosome protection mechanism. This determinant is commonly associated with the conjugative transposon Tn916 (P¡0.0002). tet(O) and tet(T) existed in a minority (2.2 % and 0.4 %, respectively). The efflux mechanism presented by tet(L) was less frequently present (4.5 %). No significant association was found between erm(B) and tet(M) genes.

show abstract

Section: Discussionsupporting

confidence: 78%

“…In the present study, target site modification mediated by the presence of the erm(B) gene was the main mechanism (97.7 %) associated with cMLSB or iMLSB, as reported in Spain (Betriu et al, 2003).…”

Section: Discussionmentioning

confidence: 73%

Macrolide and tetracycline resistance in clinical strains of Streptococcus agalactiae isolated in Tunisia

Hraoui¹,

Boubaker²,

Rachdi³

et al. 2012

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…Most of the erythromycin-resistant GBS isolated in our study (Table 3) contained ermB or ermTR. Other studies have also reported high prevalence of ermB among macrolide-resistant GBS isolates (Betriu et al, 2003;Diekema et al, 2003;Zhao et al, 2008), and ermTR was the most common macrolideresistance determinant in the USA and Canada, followed by ermB (de Azavedo et al, 2001;Dogan et al, 2005). The low prevalence of mef genes in the Kuwaiti GBS isolates is similar to those reported by others (Aracil et al, 2002;de Azavedo et al, 2001).…”

Section: B-lactamsupporting

confidence: 70%

“…This increase may be related to the increased use of macrolides in the hospital. A higher prevalence of erythromycin resistance has been reported in other studies, namely from Korea (20 %) (Uh et al, 2001), Brazil (53 %) (Betriu et al, 2003) and Taiwan (46 %) (Hsueh et al, 2001). Most of the erythromycin-resistant GBS isolated in our study (Table 3) contained ermB or ermTR.…”

mentioning

confidence: 48%

“…However, erythromycin and clindamycin resistance have emerged in several countries (Aracil et al, 2002;Betriu et al, 2003;de Azavedo et al, 2001;De Mouy et al, 2001). Erythromycin resistance in GBS isolates is due to target-site modification by rRNA methylase, encoded by the erm gene (Roberts et al, 1999), and drug efflux encoded by the mef gene (macrolide efflux), which gives rise to the M Abbreviation: GBS, group B streptococcus.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Serotypes and antibiotic resistance in Group B streptococcus isolated from patients at the Maternity Hospital, Kuwait

Boswihi

Udo

Al‐Sweih

2012

Journal of Medical Microbiology

View full text Add to dashboard Cite

A total of 143 group B streptococcus (GBS) isolates collected from mothers at the Maternity Hospital in Kuwait were investigated for their serotypes and antibiotic resistance, and screened by PCR for the carriage of genes for resistance to tetracycline (tetk, tetM, tetL, tetO), erythromycin (ermA, ermB, ermC, ermTR, ermM, mefA, mefE, msrA) and aminoglycosides (aph3, ant4, ant6). All isolates were serotyped using a latex agglutination test. Most of the isolates belonged to serotypes V (38.5 %), III (20.9 %), Ia (7.7 %) and II (11.2 %). Sixteen isolates (11.2 %) were nontypable. All isolates were susceptible to penicillin, ampicillin and cefotaxime (MICs 0.016-0.094 mg ml "1 ) but were resistant to trimethoprim (92.3 %), tetracycline (89.5 %), minocycline (89.5 %), high-level kanamycin (76.9 %), chloramphenicol (30.0 %), erythromycin (12.6 %), clindamycin (7.0 %), high-level streptomycin (3.5 %) and ciprofloxacin (0.7 %). The tetracycline-resistant isolates contained tetM (94.5 %), tetO (3.9 %), tetL (1.6 %) and tetK (0.8 %). The erythromycin-resistant isolates contained ermB (61.1 %), ermTR (38.9 %), ermA (5.5 %), mefA (5.5 %) and mefE (11 %). All high-level kanamycin-resistant isolates contained aph3. One of the high-level streptomycin-resistant isolates contained ant6. Partial DNA sequencing of aph3 revealed sequences with 99 % similarity to aph3 found in Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus and Staphylococcus epidermidis, suggesting that the GBS isolates could have acquired aph3 from other Gram-positive cocci. The high proportion of isolates with resistance to tetracycline, high-level kanamycin and trimethoprim, and the increase in the prevalence of erythromycin resistance, represents an emerging public health concern that needs further surveillance. INTRODUCTIONGroup B streptococcus (GBS), also known as Streptococcus agalactiae, is a leading cause of serious infections in neonates and immunocompromised adults (Truog et al., 1976;Baker, 1979;High et al., 2005).GBS are encapsulated and have been classified into ten serotypes (Ia, Ib, II, III, IV, V, VI, VII, VIII and IX) on the basis of the capsular polysaccharide components of their cell wall (Victor & Rubens, 2000;Slotved et al., 2007). The capsular polysaccharide, as a virulence factor, assists GBS survival in vivo by avoiding recognition by the host defence system (Rubens et al., 1987). There are major differences between countries in the distribution of GBS serotypes. In the USA, Germany and Sweden, the most common serotypes isolated from infants and colonized mothers have been types Ia, III, and V (Persson et al., 2004;Brimil et al., 2006;Harrison et al., 1998). Serotype V strains emerged in the 1990s as a frequent cause of neonatal sepsis and adult diseases in the USA. On the other hand, serotypes VI and VIII represented 35.6 % and 24.7 %, respectively, of GBS isolated from pregnant women in Japan (Lachenauer et al., 1999).Penicillin G is the drug of choice for the intrapartum prophylaxis of GBS infections (Figueira-Coelho e...

show abstract

Antibiotic Resistance of Non-Pneumococcal Streptococci and Its Clinical Impact

Jalava¹,

Seppälä

2009

Antimicrobial Drug Resistance

View full text Add to dashboard Cite

Erythromycin and Clindamycin Resistance and Telithromycin Susceptibility in Streptococcus agalactiae

Cited by 68 publications

References 19 publications

Macrolide and tetracycline resistance in clinical strains of Streptococcus agalactiae isolated in Tunisia

Macrolide and tetracycline resistance in clinical strains of Streptococcus agalactiae isolated in Tunisia

Serotypes and antibiotic resistance in Group B streptococcus isolated from patients at the Maternity Hospital, Kuwait

Antibiotic Resistance of Non-Pneumococcal Streptococci and Its Clinical Impact

Contact Info

Product

Resources

About