Erythromycin Suppresses Interleukin 6 Expression by Human Bronchial Epithelial Cells: A Potential Mechanism of Its Anti-inflammatory Action

Takizawa, Hajime; Desaki, Masashi; Ohtoshi, Takayuki; Kikutani, Takehiko; Okazaki, Hideki; Sato, Makoto; Akiyama, Norihisa; Shoji, Shuichi; Hiramatsu, Kazumasa; Ito, Kazuhiro

doi:10.1006/bbrc.1995.1727

Cited by 122 publications

(81 citation statements)

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“…As for mRNA, in contrast to the previously published report of TAKIZAWA et al [8], a decrease in the expression of cytokine mRNA was not demonstrated. In the present study, mRNA levels were slightly increased rather than decreased.…”

Section: Discussioncontrasting

confidence: 99%

“…Erythromycin suppressed the release of IL-6 in human bronchial epithelial cells [8]. Also, KHAIR et al [16] reported that erythromycin significantly blocked the Haemophilus influenzae endotoxin-induced release of IL-6, IL-8 and soluble intercellular adhesion molecule-1 from airway epithelial cells.…”

Section: Discussionmentioning

confidence: 96%

“…In the present study, mRNA levels were slightly increased rather than decreased. In the work of TAKIZAWA et al [8], a different cell types was used (bronchial epithelial cells), and the culture period with erythromycin was only 24 h. It was important that the administration of erythromycin was performed in vivo and for a long period of time in the present study. This is an essential difference between the study of TAKIZAWA et al [8] and the present one.…”

Section: Discussionmentioning

confidence: 99%

“…Although, in view of these pharmacological actions, erythromycin may function as an anti-inflammatory or immunomodulatory agent, the precise mechanism of the effects of erythromycin has not been clarified. TAKIZAWA et al [8] reported that erythromycin decreased the steady-state levels of IL-6 messenger ribonucleic acid (mRNA) expression in bronchial epithelial cells.…”

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confidence: 99%

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Effects of long-term administration of erythromycin on cytokine production in rat alveolar macrophages

Sugiyama¹,

Yanagisawa²,

Tominaga³

et al. 1999

Eur Respir J

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Effects of long-term administration of erythromycin on cytokine production in rat alveolar macrophages. Y. Sugiyama, K. Yanagisawa, S-I. Tominaga, S. Kitamura. #ERS Journals Ltd 1999. ABSTRACT: Low-dose long-term erythromycin treatment has recently been reported to be very effective in patients with chronic respiratory infection and inflammation. This effect of erythromycin was thought to be not antibacterial but anti-inflammatory. However, the exact mechanism of the effect of erythromycin has not yet been clarified. The aims of this study were to investigate the effects of erythromycin on cytokine production and its mechanisms of actions in rat alveolar macrophages.Using rats with or without administration of erythromycin for 3 months, the production of the cytokines tumour necrosis factor-a (TNF-a), cytokine-induced neutrophil chemoattractant (CINC)-1 and CINC-2a by enzyme-linked immunosorbent assay and the expression of TNF-a and CINC-1 messenger ribonucleic acid (mRNA) by Northern blotting in rat alveolar macrophages were analysed. CINC-1 is the rat counterpart of human interleukin-8, and CINC-2a of human macrophage inflammatory peptide-2.Erythromycin reduced cytokine production and secretion when cytokines was induced by lipopolysaccharide treatment. Conversely, erythromycin slightly upregulated the expression of cytokine mRNA.These results suggest that erythromycin inhibits cytokine production and exhibits anti-inflammatory effects by means of a translational and/or posttranslational mechanism.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Effects of long-term administration of erythromycin on cytokine production in rat alveolar macrophages

Sugiyama¹,

Yanagisawa²,

Tominaga³

et al. 1999

Eur Respir J

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“…Clinical studies have shown that macrolides have beneficial effects in diffuse panbronchiolitis (19), bronchial asthma (20), bronchiectasis (21), bronchiolitis obliterans syndrome (22), and cystic fibrosis (23). Furthermore, erythromycin suppresses NF-B activation and inhibits the production of IL-6 and IL-8 in human bronchial epithelial cells (24,25). In animal models, AZM attenuates the lipopolysaccharide (LPS)-induced increase in plasma TNF-␣ concentrations and improves inflammatory markers (total cell count, neutrophil percentage and macrophage inflammatory protein-2) in bronchoalveolar lavage fluid (26).…”

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confidence: 99%

Azithromycin Suppresses Activation of Nuclear Factor-kappa B and Synthesis of Pro-inflammatory Cytokines in Tracheal Aspirate Cells From Premature Infants

et al. 2007

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Nuclear factor-kappaB (NF-B) plays a central role in regulating key proinflammatory mediators. The activation of NF-B is increased in tracheal aspirate (TA) cells from premature infants developing bronchopulmonary dysplasia (BPD). We studied the effect of azithromycin (AZM) on the suppression of NF-B activation and the synthesis of pro-inflammatory cytokines IL-6 and IL-8 by TA cells obtained from premature infants. Tracheal aspirate cells were stimulated with tumor necrosis factor-alpha (TNF-␣) and incubated with AZM. The nuclear NF-B-DNA binding activity, the levels of inhibitory kappaB-alpha (IB-␣) in the cytoplasmic fraction and IL-6 and IL-8 release in the cell culture media were measured. Stimulation of TA cells by TNF-␣ increased the activation of NF-B, which was suppressed by the addition of AZM. Increased activation of NF-B was also associated with increased levels of pro-inflammatory cytokines (IL-6 and IL-8). AZM significantly reduced the IL-6 and IL-8 production to the levels similar to control. TNF-␣ stimulation also increased the degradation of IB-␣, which was restored with the addition of AZM. Our data suggest that AZM therapy may be an effective alternative to steroids in reducing lung inflammation and prevention of BPD in ventilated premature infants. (Pediatr Res 62: [483][484][485][486][487][488] 2007)

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Anti‐Infective Treatments in Asthma and COPD

Macintyre

Johnston

2012

Advances in Combination Therapy for Asthma and COPD

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Erythromycin Suppresses Interleukin 6 Expression by Human Bronchial Epithelial Cells: A Potential Mechanism of Its Anti-inflammatory Action

Cited by 122 publications

References 0 publications

Effects of long-term administration of erythromycin on cytokine production in rat alveolar macrophages

Effects of long-term administration of erythromycin on cytokine production in rat alveolar macrophages

Azithromycin Suppresses Activation of Nuclear Factor-kappa B and Synthesis of Pro-inflammatory Cytokines in Tracheal Aspirate Cells From Premature Infants

Anti‐Infective Treatments in Asthma and COPD

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