Erythropoietic changes in rats after 2.45 GHz nonthermal irradiation

Bušljeta, Ivana; Trošić, Ivančica; Milković-Kraus, Sanja

doi:10.1078/1438-4639-00326

Cited by 24 publications

(10 citation statements)

References 14 publications

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“…Polychromatic erythrocyte (PCE) count showed no signifi cant differences between RF/MWexposed and control animals on the fi nal day of our experiment. After a sharp rise at the beginning of exposure, PCE counts normalised by the end of the experiment thanks to an adaptive mechanism involved in cell proliferation and maturation (15,16). These results are consistent with our fi ndings of a transitory infl ux of immature erythrocytes into the peripheral circulation in rats exposed to sub-chronic 2.45 GHz irradiation (17).…”

Section: Rf/mw Influence On the Haematopoiesis In Ratssupporting

confidence: 82%

Non-Thermal Biomarkers of Exposure to Radiofrequency/Microwave Radiation

Trošić¹,

Pavičić²,

Marjanović³

et al. 2012

Archives of Industrial Hygiene and Toxicology

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This article gives a review or several hypotheses on the biological effects of non-thermal radiofrequency/ microwave (RF/MW) radiation and discusses our own fi ndings from animal and in vitro studies performed over the last decade. We have found that RF/MW radiation disturbs cell proliferation and leads to cell differentiation in the bone marrow, which is refl ected in the peripheral blood of rats. Repeated RF/MW radiation can also temporarily disrupt melatonin turnover. The observed changes seem to be a sign of adaptation to stress caused by irradiation rather than of malfunction. The article looks further into the basic mechanisms of RF/MW biological action, including cell growth parameters, colony-forming ability, viability, and the polar and apolar protein cytoskeleton structures. The observed reversible cell changes signifi cantly obstructed cell growth. In contrast to the apolar intermediate proteins, the intracellular polar microtubule and actin fi bres were damaged by radiation in a time-dependent manner. These signifi cantly altered parameters can be considered as the biomarkers of exposure. Future research should combine dosimetry, experimental studies, and epidemiological data.

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Section: Rf/mw Influence On the Haematopoiesis In Ratssupporting

confidence: 82%

Non-Thermal Biomarkers of Exposure to Radiofrequency/Microwave Radiation

Trošić¹,

Pavičić²,

Marjanović³

et al. 2012

Archives of Industrial Hygiene and Toxicology

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“…Recently, Trosic and coworkers showed that rat blood forming system could be affected by low level RF/MW fields (Trosic et al, 2001;2004a,b). In addition, there have been a number of biological effects observed in animals during the radiofrequency field at non-thermal level exposure (Repacholi et al, 1997;Trosic et al, 2002;Busljeta et al, 2004). Previous studies in the field of mobile phones frequencies often presented results that have been contradictory, and thus confusing to the readers.…”

Section: Introductionmentioning

confidence: 81%

Influence of 864 MHz electromagnetic field on growth kinetics of established cell line

Pavičić

Trošić

2006

Biologia

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Considering often contradictory data on biological effects of mobile phones frequencies on established cell culture lines, our study aimed at evaluating the influence of 864 MHz electromagnetic field on proliferation, colony forming ability and viability of Chinese hamster lung cells continuous line V79. Prior to exposure for 1, 2 and 3 hours in transversal electromagnetic mode cell (TEM-cell) equipped by Philips PM 5508 signal generator cell samples were sub-cultivated for one day. Cell samples were exposed to 864 MHz continuous wave at an average specific absorption rate (SAR) of 0.08 W/kg. To determine cell growth, V79 cells were plated in concentration of 1 × 10 4 cells per milliliter of nutrient medium RPMI 1640, and raised in a humified atmosphere at 37• C in 5% CO2. Cell proliferation was determined by cell counts for each hour of exposure on post-exposure day 1, 2, 3, 4 and 5. To identify colony-forming ability, cells were cultivated in concentration of 40 cells/mL of RPMI 1640 and incubated according to the deliberated experimental protocol. Colony forming ability for each hour of exposure was defined by colony counts on experimental day 7. Trypan blue exclusion test was used to determine viability of cells. In comparison to sham-exposed cells, growth curve of irradiated cell samples showed significant decrease (p < 0.05) after 2 and 3 hours of exposure on experimental day 3, respectively. Both, the colony forming ability and viability of irradiated cells did not significantly differ from exposed "mock" condition. Under strictly controlled laboratory conditions, applied radiofrequency microwaves (RF/MW) irradiation significantly affected cell proliferation kinetics but not viability or ability of V79 cells to form colonies. Sophisticated mechanism of action is intending to be elucidated in the further research which will include insight into the RF/MW related event at the subcellular level.

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“…Also, Busljeta et al (2004) revealed that exposure of adult male rats to 2.45 GHz usually resulted a significant increase in micronucleus formation in peripheral blood and that continuous radiofrequency/microwave field for 2 h daily, 7 d a week might cause disturbance in red cell maturation and proliferation, as well as induce micronucleus formation in erythropoietin cells.…”

Section: Micronucleus Test and Emws Emitted From Mphbs And Mphmentioning

confidence: 99%

Effect of Electromagnetic Waves on the Frequency of Micronucleated Polychromatic Erythrocytes and the Sperm Morphology of Rats (<i>Rattus norvegicus</i>)

2012

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Every year, millions of people are added to the total number of mobile telephone users. The convenience of this device is indisputable but its safety remains inconclusive. Questions are still asked all around the world about mobile telephone safety and whether these phones pose a hazard to health. Electromagnetic waves (EMWs) are made up of linked electric and magnetic components moving together through space at the speed of light. The electric and magnetic components that form EMWs can be referred to as radiofrequency fields. The present work searched for the cytogenetic effect of EMWs emitted from mobile telephones and their relay stations. This was achieved using Micronucleated polychromatic erythrocytes (MNPCEs) and an examination of the sperm abnormality of rats. The data obtained revealed a marked increase in the frequency of micronucleated polychromatic erythrocyte cells that were taken from bone marrow cells and sperm abnormality from the testes of rats exposed for 2 weeks to electromagnetic waves emitted from global systems for mobile telephone communication base stations and mobile telephone systems. The obtained results were significant at the statistical level. In addition, the frequency of micronucleus formation was more affected in the group of rats exposed to mobile telephone systems than others and remained high in the recovery group. Also, the frequency of sperm abnormality was more sensitive in the day group of telephone base station rats than others. In the present work, the results indicated that prolonged exposure to the EMWs emitted from global systems for mobile communication base stations or cellular mobile telephone communication causes clastogenic effects at the level of MNPCEs as well as abnormally shaped sperm.

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Erythropoietic changes in rats after 2.45 GHz nonthermal irradiation

Cited by 24 publications

References 14 publications

Non-Thermal Biomarkers of Exposure to Radiofrequency/Microwave Radiation

Non-Thermal Biomarkers of Exposure to Radiofrequency/Microwave Radiation

Influence of 864 MHz electromagnetic field on growth kinetics of established cell line

Effect of Electromagnetic Waves on the Frequency of Micronucleated Polychromatic Erythrocytes and the Sperm Morphology of Rats (<i>Rattus norvegicus</i>)

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