Erythropoietin and Its Angiogenic Activity

Kimáková, Patrícia; Solár, Peter; Solárová, Zuzana; Komel, Radovan; Debeljak, Nataša

doi:10.3390/ijms18071519

Cited by 94 publications

(67 citation statements)

References 99 publications

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“…We conclude that the virulence of SARS-CoV-2 is reduced at high-altitude due to the physiological acclimatization of its inhabitants, and due to particular environmental characteristics. Furthermore, additional physiological acclimatization of high-altitude living associated with increased ventilation (Soliz et al 2005), augmented arterial oxygen transport (Lundby et al 2007), and higher tissue oxygenation (Kimakova et al 2017), mainly (but not exclusively) mediated by erythropoietin could be explored for potential therapy (see Soliz et al, 2020, same issue RSPNB) of acute respiratory distress associated with COVID-19.…”

Section: Discussionmentioning

confidence: 99%

Does the pathogenesis of SARS-CoV-2 virus decrease at high-altitude?

Arias‐Reyes

Zubieta-DeUrioste

Poma-Machicao

et al. 2020

Respiratory Physiology & Neurobiology

204

224

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Does the pathogenesis of SAR-CoV-2 virus decrease at high-altitude?, Respiratory Physiology and amp; Neurobiology (2020), doi: https://doi. J o u r n a l P r e -p r o o fHighlights  COVID-19 infection is decreased in populations living at an altitude of above 3,000 masl  Highland inhabitants may be less susceptible to SARS-CoV-2 virus infection due to physiological acclimatization to hypoxia  High-altitude environmental factors may contribute to reduce the virulence of SARS-CoV-2 ABSTRACTIn the present study we analyze the epidemiologic data of COVID-19 of Tibet and high-altitude regions of Bolivia and Ecuador, and compare to lowland data, to test the hypothesis that high-altitude inhabitants (+2500 m above sea-level) are less susceptible to develop severe adverse effect in acute SARS-CoV-2 virus infection. Analysis of available epidemiological data suggest that physiological adaptations that counterbalance the hypoxic environment altitude may protect from severe impact of acute SARS-CoV-2 virus infection. Potential underlying mechanisms such as: (i) a compromised half-live of the virus caused by the high-altitude environment, and (ii) a hypoxia mediated down regulation of angiotensin-converting enzyme 2 (ACE2), which is the main binding target of SARS-CoV-2 virus in the pulmonary epithelia are discussed.

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Section: Discussionmentioning

confidence: 99%

Does the pathogenesis of SARS-CoV-2 virus decrease at high-altitude?

Arias‐Reyes

Zubieta-DeUrioste

Poma-Machicao

et al. 2020

Respiratory Physiology & Neurobiology

204

224

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“…The rapid establishment of a sufficient vascularisation is crucial for the in vivo survival and long-term function of tissue-engineered constructs (Laschke et al, 2006;Novosel et al, 2011). Since the ingrowth of blood vessels from the surrounding host tissue into the implants is a time-consuming angiogenic process, sophisticated pre-vascularisation strategies have recently been developed (Muscari et al, 2014;Laschke and Menger, 2016a).…”

Section: Introductionmentioning

confidence: 99%

Erythropoietin promotes network formation of transplanted adipose tissue-derived microvascular fragments

Karschnia¹,

Scheuer²,

Heß³

et al. 2018

eCM

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The seeding of tissue constructs with adipose tissue-derived microvascular fragments (ad-MVF) is an emerging pre-vascularisation strategy. Ad-MVF rapidly reassemble into new microvascular networks after in vivo implantation. Herein it was analysed whether this process was improved by erythropoietin (EPO). Ad-MVF were isolated from green fluorescent protein (GFP)+ as well as wild-type C57BL/6 mice and cultivated for 24 h in medium supplemented with EPO (20 IU/mL) or vehicle. Freshly isolated, non-cultivated ad-MVF served as controls. Protein expression, cell viability and proliferation of ad-MVF were assessed by proteome profiler array and fluorescence microscopy. GFP+ ad-MVF were seeded on collagen-glycosaminoglycan matrices, which were implanted into dorsal skinfold chambers of C57BL/6 mice, to analyse their vascularisation over 14 d by intravital fluorescence microscopy, histology and immunohistochemistry. Cultivation up-regulated the expression of pro- and anti-angiogenic factors within both vehicle- and EPO-treated ad-MVF when compared with non-cultivated controls. Moreover, EPO treatment suppressed cultivation-associated apoptosis and significantly increased the number of proliferating endothelial cells in ad-MVF when compared with vehicle-treated and non-cultivated ad-MVF. Accordingly, implanted matrices seeded with EPO-treated ad-MVF exhibited an improved vascularisation, as indicated by a significantly higher functional microvessel density. The matrices of the three groups contained a comparably large fraction of GFP+ microvessels originating from the ad-MVF, whereas the tissue surrounding the matrices seeded with EPO-treated ad-MVF exhibited a significantly increased microvessel density when compared with the other two groups. These findings indicated that EPO represents a promising cytokine to further boost the excellent vascularisation properties of ad-MVF in tissue-engineering applications.

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“…Of interest for skeletal tissue engineering, the pleiotropic capabilities of EPO include osteogenic and angiogenic potencies. (6)(7)(8)(9)(10) The studies coupling the EPO to bone healing reported that the use of EPO was supraphysiologic dosage and had potential harmful implication like high blood viscosity and consecutive thromboembolic events. Many researches shifted to the use of topical administration of EPO into the bone defect to improve the efficiency of EPO on the bone formation with minimal systemic effect.…”

Section: Discussionmentioning

confidence: 99%

Effects of Erythropoietin on The Healing of Calvarial Bone Defect

Ahmed

Elmagd

Khairy

et al. 2019

Egyptian Dental Journal

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Objectives To evaluate the effect of a single-dose local administration versus the systemic administration of Erythropoietin on bone healing in calvarial bone defectsMaterial & Methods Critical-size cranial osteotomy defects were created in 30 rabbits. The animals were randomly divided into 3 groups. The animals were randomly divided into 3 groups (n= 10 animals in each group). In the Group I, the bone defect was only filled with a collagen sponge soaked with erythropoietin. In the Group II, it was filled with a collagen sponge and erythropoietin injected systemically. While in the Group III, the defect was filled with a collagen sponge. The groups were further split in two for euthanasia 10-and 21-days post-surgery. New bone formation and neovascularization were evaluated by hematoxylin-eosin. For the 10-days samples, all the groups analyzed for area percent of blood vessels while on the 21-day samples, the area of new bone formation was calculated. Differences between groups were analyzed by one-way ANOVA.Results At 10 days post-surgery, the histological analysis showed that the erythropoietin Groups exhibited a significantly higher percentage of bone formation compared with the other Group. At 21 days post-surgery, a higher percentage of new bone was observed in the erythropoietin group. ConclusionsThe results suggest that both local and systemic administration of erythropoietin hormone encouraged the bone healing in critical-size calvarial defects in Rabbits.

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Erythropoietin and Its Angiogenic Activity

Cited by 94 publications

References 99 publications

Does the pathogenesis of SARS-CoV-2 virus decrease at high-altitude?

Does the pathogenesis of SARS-CoV-2 virus decrease at high-altitude?

Erythropoietin promotes network formation of transplanted adipose tissue-derived microvascular fragments

Effects of Erythropoietin on The Healing of Calvarial Bone Defect

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