Erythropoietin non-viral gene therapy does not affect motility, viability, morphology or concentration of rabbit sperm

Collares, Tiago; Campos, Vinícius Farias; Urtiaga, G.; Leon, Priscila Marques Moura de; Amaral, Marta; Hartleben, Cláudia Pinho; McBride, Alan J. A.; Dellagostin, Odir A.; Deschamps, João Carlos; Seixas, Fabiana Kömmling

doi:10.1017/s1751731112002157

Cited by 3 publications

(2 citation statements)

References 21 publications

(29 reference statements)

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“…In another experimental study using adult rats with testicular torsion/detorsion, in vivo administration of an EPO analogue prevented testicular damage and reduced oxidative makers again, suggesting that EPO may be acting as a direct and/or indirect antioxidant (27). On the other hand, the EPO gene transfer or EPO administration in male rabbits had no significant effects on sperm concentration, motility or vitality (28).…”

Section: Discussionmentioning

confidence: 97%

Erythropoitein IncreasesIn VitroMotility and Vitality of Human Spermatozoa

Asimakopoulos

Tiptiri-Kourpeti

Metalinou

2021

In Vivo

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Background/Aim: Erythropoietin and its receptor are expressed in the male reproductive system. Initial studies have shown that erythropoietin affects the motility of spermatozoa. The aim of the present study was to investigate the in vitro effect of erythropoietin in the motility and vitality of human spermatozoa. Materials and Methods: Forty-three semen samples, obtained after 2-4 days of abstinence from sex, were analyzed and processed using density gradient centrifugation. Aliquots containing one million of spermatozoa were treated with either erythropoietin, at concentrations of 10 and 100 mIU/μl or standard culture medium for one hour. Results: Progressive motility and vitality of spermatozoa significantly increased following treatment with erythropoietin. The effect was not dosedependent. Conclusion: The supplementation of culture medium with erythropoietin improves sperm processing in terms of vitality and motility. Future research should focus on the effects of erythropoietin on sperm capacitation as well as on the signal transduction pathways activated by erythropoietin and its receptor in spermatozoa.

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Section: Discussionmentioning

confidence: 97%

Erythropoitein IncreasesIn VitroMotility and Vitality of Human Spermatozoa

Asimakopoulos

Tiptiri-Kourpeti

Metalinou

2021

In Vivo

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“…Further, in rats, Collares et al, 2012, constructed an EPO expression vector and transferred it to male rabbits via intramuscular injection; they examined the effects of nonviral EPO gene transfer and intravenous rHuEPO administration on sperm motility, viability, morphology, and concentration [97]. It appears that the EPO gene transfer and rHuEPO administration do not affect the sperm characteristics in the rat testis.…”

Section: Epo In the Male Reproductive System Of Animal Modelsmentioning

confidence: 99%

NGF, EPO, and IGF-1 in the Male Reproductive System

Metallinou,

Staneloudi,

Nikolettos

et al. 2024

JCM

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Several studies have demonstrated interesting results considering the implication of three growth factors (GFs), namely nerve growth factor (NGF), erythropoietin (EPO), and the insulin-like growth factor-I (IGF-1) in the physiology of male reproductive functions. This review provides insights into the effects of NGF, EPO, and IGF-1 on the male reproductive system, emphasizing mainly their effects on sperm motility and vitality. In the male reproductive system, the expression pattern of the NGF system varies according to the species and testicular development, playing a crucial role in morphogenesis and spermatogenesis. In humans, it seems that NGF positively affects sperm motility parameters and NGF supplementation in cryopreservation media improves post-thaw sperm motility. In animals, EPO is found in various male reproductive tissues, and in humans, the protein is present in seminal plasma and testicular germ cells. EPO receptors have been discovered in the plasma membrane of human spermatozoa, suggesting potential roles in sperm motility and vitality. In humans, IGF-1 is expressed mainly in Sertoli cells and is present in seminal plasma, contributing to cell development and the maturation of spermatozoa. IGF-1 seems to modulate sperm motility, and treatment with IGF-1 has a positive effect on sperm motility and vitality. Furthermore, lower levels of NGF or IGF-1 in seminal plasma are associated with infertility. Understanding the mechanisms of actions of these GFs in the male reproductive system may improve the outcome of sperm processing techniques.

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The Role of Erythropoietin in Bovine Sperm Physiology

Sapanidou,

Asimakopoulos,

Lialiaris

et al. 2024

Animals

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Erythropoietin (EPO), a hormone secreted mainly by the kidney, exerts its biological function by binding to its cell-surface receptor (EpoR). The presence of EPO and EpoR in the male and female reproductive system has been verified. Therefore, some of the key properties of EPO, such as its antioxidant and antiapoptotic effects, could improve the fertilizing capacity of spermatozoa. In the present study, the effect of two different concentrations of EPO (10 mIU/μL and 100 mIU/μL) on bovine sperm-quality parameters was evaluated during a post-thawing 4-h incubation at 37 °C. EPO had a positive effect on sperm motility, viability, and total antioxidant capacity. Moreover, EPO inhibited apoptosis, as it reduced both BCL2-associated X apoptosis regulator (Bax)/B-cell lymphoma 2 (Bcl-2) ratio and cleaved cysteine-aspartic proteases (caspases) substrate levels in a dose-dependent manner. In addition, EPO induced sperm capacitation and acrosome reaction in spermatozoa incubated in capacitation conditioned medeia. These results establish a foundation for the physiological role of EPO in reproductive processes and hopefully will provide an incentive for further research in order to fully decipher the role of EPO in sperm physiology and reproduction.

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Erythropoietin non-viral gene therapy does not affect motility, viability, morphology or concentration of rabbit sperm

Cited by 3 publications

References 21 publications

Erythropoitein IncreasesIn VitroMotility and Vitality of Human Spermatozoa

Erythropoitein IncreasesIn VitroMotility and Vitality of Human Spermatozoa

NGF, EPO, and IGF-1 in the Male Reproductive System

The Role of Erythropoietin in Bovine Sperm Physiology

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