Erythropoietin Sensitivity of Rat Bone Marrow Cells Separated by Velocity Sedimentation

McCool, D.; Miller, R.; Painter, R. H.; Bruce, W. R.

doi:10.1111/j.1365-2184.1970.tb00252.x

Cited by 20 publications

(9 citation statements)

References 8 publications

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“…lb, c). These results, therefore, in general confirm the studies of other work ers [2,8,9,11,15,18] that several distinct Ep-sensitive cell populations can be separated from suspensions of various hemopoietic tissues. The differences in the absolute sedimentation rates may reflect tissue-specific cell sizes or minor differences in technique.…”

Section: Discussionsupporting

confidence: 82%

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Separation of Erythropoietin Responsive Cells in Fetal Mouse Liver

Dunn¹,

Napier²,

Ford³

et al. 1978

Acta Haematol

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The erythropoietin responsive cells (ERC) in suspension cultures (ERC_SC) of fetal mouse liver and the cells producing erythroid colonies in 2-day plasma clot cultures (CFU-E) sediment at similar rates. However, the sedimented fractions containing the majority of nucleated cells show minimal sensitivity to erythropoietin (Ep) and these cells sediment at a slower rate than the ERC_SC. The studies suggest that in short-term suspension cultures of fetal mouse liver, Ep acts on morphologically unrecognizable cells to increase the number of hemoglobin-synthesizing cells rather than by increasing the quantity of hemoglobin synthesized per cell. This effect is similar to the principal in vivo action of Ep.

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Section: Discussionsupporting

confidence: 82%

“…These cells are descended from the 'multipotential stem cell' and precede morphologically recogniz able erythropoietic cells [1]. In 1970, McCool et al [11] were able to separate, on the basis of cell size, a population of ERC in suspension cul tures (ERCst.) from the majority of nucleated cells in rat bone marrow.…”

Section: Introductionmentioning

confidence: 99%

Separation of Erythropoietin Responsive Cells in Fetal Mouse Liver

Dunn¹,

Napier²,

Ford³

et al. 1978

Acta Haematol

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“…Separation oftarget cells by velocity sedimentation suggests that L-T4 affects a population that is somehow distinct from the majority of ESF-responsive progenitors. Inasmuch as the method separates cells primarily according to their size (20), such separation may reflect differences in maturation (24) and (or) stage ofcell cycle (25). These observations support the contention that the erythroid progenitor cell responding to thyroid hormone may represent a subpopulation of erythroid colony-forming units; whether it is more or less differentiated than the majority of the ESF-responsive cells is not known.…”

Section: Discussionmentioning

confidence: 99%

The Influence of Thyroid Hormones on In Vitro Erythropoiesis

Popovic¹,

Brown²,

Adamson³

1977

J. Clin. Invest.

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A B S T R A C T The erythropoietic effect of various thyroid hormones has been studied using erythroid colony formation by canine marrow cells. Although erythropoietin was required for colony growth, physiologic levels ofthyroid hormones significantly enhanced colony numbers. The order of potency of the thyroid compounds in their in vitro erythropoietic effect parallels their known calorigenic potency in vivo, suggesting that the in vitro effect is physiologically relevant. A series ofstudies linked the mechanism ofthyroid action to adrenergic receptors on responsive cells. Propranolol, a global ,8-blocker, inhibited thyroid hormoneresponsive erythroid colonies. When adrenergic antagonists having different blocking characteristics were added to culture, the thyroid hormone effect was blocked by those compounds having ,82-subspecificity.Velocity sedimentation analysis showed that the peak of colony-forming cells which respond to thyroid hormone and the adrenergic agonist, isoproterenol, sedimented at an identical rate (7.54 mm/h), which is slower than the major peak ofcolony-forming cells responding to erythropoietin alone (8.62 mm/h). These results demonstrate thyroid hormonal enhancement of in vitro erythroid colony growth which appears mediated by a receptor with (82-adrenergic properties.

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“…The present data do not exclude such an effect, but an accelerated rate of maturation does not, alone, adequately explain the observed preservation of replicating precursor 594 THE JOURNAL OF CELL BIOLOGY • VOLUME 51,1971 cells in the hormone-treated cultures . Recent studies (McCool et al ., 1970) employing fractionated cell populations likewise suggested that stimulation of a hemoglobinizing population of maturing erythroid cells constitutes at best a minor portion of the erythropoietic response to erythropoietin .…”

Section: Thymidine-1 H Labeling Indexmentioning

confidence: 99%

Erythropoietin Effects on Fetal Mouse Erythroid Cells

Chui

Djaldetti

Marks

et al. 1971

The Journal of Cell Biology

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The effect of the hormone, erythropoietin, on cultures of erythroblasts derived from the livers of fetal C57BL/6J mice was examined . An increase both in the content and in the rate of synthesis of normal adult mouse globin chains was detected in hormone-treated cultures . The rate of protein synthesis by individual erythroblasts does not increase in response to the hormone, whereas the absolute number of hemoglobin-synthesizing cells does increase and accounts for the observed stimulation of hemoglobin synthesis . The principal effect of erythropoietin appears to be upon the population of immature erythroid precursor cells which persists in the presence of the hormone, the cells maintaining their ability to replicate, and their capacity to differentiate into hemoglobinizing erythroblasts . In the absence of hormone, already committed erythroblasts continue their development, but erythropoiesis is not sustained .

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Erythropoietin Sensitivity of Rat Bone Marrow Cells Separated by Velocity Sedimentation

Cited by 20 publications

References 8 publications

Separation of Erythropoietin Responsive Cells in Fetal Mouse Liver

Separation of Erythropoietin Responsive Cells in Fetal Mouse Liver

The Influence of Thyroid Hormones on In Vitro Erythropoiesis

Erythropoietin Effects on Fetal Mouse Erythroid Cells

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