ErZhiFormula prevents UV-induced skin photoaging by Nrf2/HO-1/NQO1 signaling: An in vitro and in vivo studies

Liu, Tao; Xia, Qingmei; Lv, Yingshuang; Wang, Zijing; Zhu, Shan; Qin, Wenxiao; Yang, Yi; Liu, Tao; Wang, Xiang; Zhao, Zhiyue; Ma, Hongfei; Jia, Linlin; Zhang, Han; Xu, Zongpei; Li, Nan

doi:10.1016/j.jep.2022.115935

Cited by 8 publications

(6 citation statements)

References 89 publications

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“…Under UVB induction, HaCaT cells exhibit an overproduction of ROS, disrupting the redox equilibrium and diminishing the activity of antioxidative enzymes [ 59 ]. The accumulation of ROS and oxidized metabolites results in intracellular lipid peroxidation, wherein MDA, an end product of lipid peroxidation, compromises the structure and function integrality of cell membranes [ 60 ]. Consequently, antioxidant therapy has become a promising approach to counteract oxidative stress and enhance skin cell functionality by mitigating ROS-induced damage.…”

Section: Discussionmentioning

confidence: 99%

Chemical Characterization of Honeysuckle Polyphenols and Their Alleviating Function on Ultraviolet B-Damaged HaCaT Cells by Modulating the Nrf2/NF-κB Signaling Pathways

Zheng,

Wang,

Chi

et al. 2024

Antioxidants

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Scientific evidence attests that the epidermis receives excessive ultraviolet B (UVB) radiation, triggering the generation of substantial quantities of reactive oxygen species (ROS), which disrupted the delicate equilibrium of oxidation–reduction, leading to oxidative stress and inflammation. The historical use of honeysuckle polyphenols (HPs) has garnered our attention due to their efficacy in inhibiting oxidative damage. In this study, HPs were prepared from honeysuckle flowers employing an ultrasonic-assisted extraction method and quantitatively analyzed by a LC–MS/MS, and the mechanisms underlying HPs’ antioxidative and anti-inflammatory effects on a UVB-irradiated HaCaT cell model were systematically investigated. The results showed that HPs had a significant cellular repair effect on UVB-irradiated HaCaT cells (p < 0.001). The mechanism of action indicated that HPs could allow Nrf2 to enter the nucleus by regulating the dissociation of Nrf2 from Keap1, which further increases the activity of downstream proteases (SOD and CAT), increases ROS scavenging, and reduces the intracellular malondialdehyde (MDA) level. In addition, HPs could down-regulate Toll-like receptor 4 (TLR4) and inhibit NF-κB (P65) dissociating from IκBα, resulting in a decrease in NF-κB (P65) entry into the nucleus and a decrease in inflammatory factors (TNF-α, IL-6, and IL-1β). In addition, four key compounds in HPs, including chlorogenic acid, quercetin, isorhamnetin, and luteolin, were selected to verify the mechanism of HPs repairing UVB damage using molecular docking techniques. The experiment suggested that four key active compounds could effectively occupy the Kelch homologue (Kelch) structural domain of Keap1, competitively bind with Nrf2, and facilitate the promotion of Nrf2 binding, ultimately enhancing the translocation of Nrf2 into the nucleus. In addition, four key active compounds could effectively interact with NF-κB (P65) through hydrogen bonding, van der Waals forces, and electrostatic forces to inhibit its entry into the nucleus. In summary, HPs can effectively repair the damage of HaCaT cells by UVB radiation and can be used to develop health and cosmetic products for the treatment of UV radiation-induced diseases.

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Section: Discussionmentioning

confidence: 99%

Chemical Characterization of Honeysuckle Polyphenols and Their Alleviating Function on Ultraviolet B-Damaged HaCaT Cells by Modulating the Nrf2/NF-κB Signaling Pathways

Zheng,

Wang,

Chi

et al. 2024

Antioxidants

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“…UV radiation is one of the important environmental factors that causes skin photoaging, which accounts for about 80% of skin aging [ 48 , 49 , 50 ]. Prolonged skin exposure to UV radiation can induce detrimental intracellular physiological effects and produce superfluous ROS, which can injury intracellular bioactive molecules, such as DNA, enzymes and proteins, and membrane lipids, and further cause oxidative stress and promote cell apoptosis [ 51 , 52 , 53 ]. Then, inhibiting photoaging induced by UVB can delay skin aging and provides a reasonable basis for studying cosmetic products to treat diseases caused by UV radiation [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

“…With the accumulation of ROS and oxidative metabolites, lipid peroxidation occurs in cells. As one of the end products of lipid peroxidation, MDA can damage the structural and functional integrity of cell membranes [ 48 , 53 ]. The intervention of antioxidant therapy is identified as a potential approach to constrain oxidative stress and improve skin cell function by alleviating ROS damage [ 50 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

Bioactive Peptides from Skipjack Tuna Cardiac Arterial Bulbs (II): Protective Function on UVB-Irradiated HaCaT Cells through Antioxidant and Anti-Apoptotic Mechanisms

Kong

Cai

et al. 2023

Marine Drugs

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The aim of this study was to investigate the protective function and mechanism of TCP3 (PKK), TCP6 (YEGGD) and TCP9 (GPGLM) from skipjack tuna cardiac arterial bulbs on skin photoaging using UVB-irradiated HaCaT cell model. The present results indicated that TCP3 (PKK), TCP6 (YEGGD) and TCP9 (GPGLM) had significant cytoprotective effect on UVB-irradiated HaCaT cells (p < 0.001). Hoechst 33342 staining showed that apoptosis of UV-irradiated HaCaT cells could be significantly reduced by the treatment of TCP3 (PKK), TCP6 (YEGGD) and TCP9 (GPGLM); JC-1 staining showed that TCP3 (PKK), TCP6 (YEGGD) and TCP9 (GPGLM) could protect HaCaT cells from apoptosis by restoring mitochondrial membrane potential (MMP); Furthermore, TCP3 (PKK), TCP6 (YEGGD) and TCP9 (GPGLM) could significantly down-regulate the ratio of Bax/Bcl-2 and reduce the expression level of the apoptosis-executing protein Caspase-3 by decreasing the expression of protein Caspase-8 and Caspase-9 (p < 0.05). The action mechanism indicated that TCP3 (PKK), TCP6 (YEGGD) and TCP9 (GPGLM) could up-regulate the expression levels of Nrf2, NQO1 and HO-1 (p < 0.05), which further increased the activity of downstream proteases (SOD, CAT and GSH-Px), and scavenged reactive oxygen species (ROS) and decreased the intracellular levels of malondialdehyde (MDA). In addition, molecular docking indicated that TCP3 (PKK) and TCP6 (YEGGD) could competitively inhibit the Nrf2 binding site because they can occupy the connection site of Nrf2 by binding to the Kelch domain of Keap1 protein. TCP9 (GPGLM) was inferred to be non-competitive inhibition because it could not bind to the active site of the Kelch domain of Keap1 protein. In summary, the antioxidant peptides TCP3 (PKK), TCP6 (YEGGD) and TCP9 (GPGLM) from cardiac arterial bulbs of skipjack tuna can effectively protect HaCaT cells from UVB-irradiated damage and can be used in the development of healthy and cosmetic products to treat diseases caused by UV radiation.

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“…When cells are stimulated by various external factors, several ROS reactions are triggered in the body. 5 The human body requires a significant amount of energy to maintain homeostasis and autonomous function. Cellular homeostasis is maintained by using mitochondria to generate large amounts of adenosine triphosphate (ATP).…”

Section: Introductionmentioning

confidence: 99%

Exogenous foods that activate the NRF2 protein: Systematic review

Esteves Pairazaman,

Caldas Herrera,

Collanque Pinto

et al. 2024

F1000Res

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Background The activation of the Nuclear factor erythroid 2-related factor 2 (NRF2) signaling pathway, whether through natural compounds, diet, or supplements, plays a crucial role in protecting against oxidative stress and promoting health in various aspects. The objective of this research was to identify studies on exogenous foods that activate the NRF2 protein in the scientific literature. Methods A literature review aimed at identifying manuscripts on NRF2 activation with exogenous foods and its human benefits was conducted. The systematic review spanned English and Spanish articles from MEDLINE, PubMed, SciELO, and Scopus repositories until August 2023. All authors participated in search, analysis, and selection. Inclusion criteria prioritized NRF2-activating foods and their benefits, with exclusion criteria for irrelevant articles in other languages. Stringent filters were applied in stages—initial title search, abstract review, and thorough reading of remaining articles—to minimize bias and ensure relevance to the study’s objective. Results A total of 25 articles were analyzed, which discussed the adaptive mechanism of NRF2 and its role in hormone dose response, the effects of certain foods and health benefits associated with NRF2 activation Conclusions The studies support the notion that NRF2 activation plays a fundamental role in promoting health in various spheres, underscoring the relevance of factors such as diet, natural components, and antioxidant supplementation in defending against oxidative stress and its implications for overall well-being. For future research on this topic, it is important to analyze how the population stands regarding the consumption of exogenous foods that activate NRF2, to understand both the consumption, quantity, and frequency of certain foods and whether they are doing so correctly.

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ErZhiFormula prevents UV-induced skin photoaging by Nrf2/HO-1/NQO1 signaling: An in vitro and in vivo studies

Cited by 8 publications

References 89 publications

Chemical Characterization of Honeysuckle Polyphenols and Their Alleviating Function on Ultraviolet B-Damaged HaCaT Cells by Modulating the Nrf2/NF-κB Signaling Pathways

Chemical Characterization of Honeysuckle Polyphenols and Their Alleviating Function on Ultraviolet B-Damaged HaCaT Cells by Modulating the Nrf2/NF-κB Signaling Pathways

Bioactive Peptides from Skipjack Tuna Cardiac Arterial Bulbs (II): Protective Function on UVB-Irradiated HaCaT Cells through Antioxidant and Anti-Apoptotic Mechanisms

Exogenous foods that activate the NRF2 protein: Systematic review

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