ESBL-Positive Enterobacteria Isolates in Drinking Water

Boeck, Hilde De; Miwanda, Berthe; Lunguya-Metila, Octavie; Muyembe‐Tamfum, Jean‐Jacques; Stobberingh, Ellen E.; Glupczynski, Youri; Jacobs, Jan

doi:10.3201/eid1806.111214

Cited by 49 publications

(27 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Many households get water from unhealthy streams used as a dumping ground by residents. A study carried out by De Boeck in Kinshasa (Central Africa ) revealed the presence of ESBL producing Enterobacteriaceae in drinking water [26] and another study revealed the presence of E. coli in rivers and wells. [21] In a village community in South Guyana, it was also proven that even in the absence of a strong antibiotic pressure, promiscuity promoted the circulation of resistant strains and thus increased the prevalence of carrying them.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of CTX-M beta-Lactamases in Escherichia coli from community-acquired urinary tract infections and associated risk factors among women in Cameroon

Djuikoue

Njajou

Kamga

et al. 2017

JER

View full text Add to dashboard Cite

Background: In recent years, a worldwide dissemination of CTX-M beta-lactamase-type in Escherichia coli strains isolates from community-acquired urinary tract infections (CA-UTI) has been observed. However little is known on the prevalence and risk factors of this global threat in developing countries. Objective: The aim of this study was to study the prevalence and risk factors for CA-UTI in Yaoundé, Cameroon. Methods: Eighty six patients with urinary E.coli infection recruited from 10 health structures in the town of Yaoundé, Cameroon. After taking the first urine, faeces were collected from the patients for the study of the intestinal flora. The sample collection of faeces was done on a selected gel of enterobacteria resistant to third generation of cephalosporin. The molecular typing of extended-spectrum β-lactamase (ESBL) was carried out. Results: Eighty-six strains of E. coli from 86 patients were included. We found that 39 (45.3%) strains produced an extendedspectrum beta-lactamase. Among risk factors, previous use of antibiotic and the dry season were associated with the presence of an ESBL-producing strain in the urine. All ESBL were identified as CTX-M. The production of CTX-M was found to be significantly associated with resistance to fluoroquinolones, aminoglycosides and to the association of trimethoprim-sulfamethazole. Conclusions: The prevalence of CTX-M ESBL in Yaoundé, Cameroon, provides new evidence on the global dissemination of CTX-M and the extent of this phenomenon in developing countries.

show abstract

Section: Discussionmentioning

confidence: 99%

Prevalence of CTX-M beta-Lactamases in Escherichia coli from community-acquired urinary tract infections and associated risk factors among women in Cameroon

Djuikoue

Njajou

Kamga

et al. 2017

JER

View full text Add to dashboard Cite

show abstract

“…We found no link between increased resistance abundance and the use of antimalaria agents but acknowledge that our study might be too small for detecting such an effect. Another reason for enrichment of resistance genes may be ingestion of resistant bacteria through food (52) or contaminated water (53)(54)(55) or by close contact with an environment containing antibiotic-resistant bacteria. The culture results provide support for the conclusion that bacteria carrying resistance genes were indeed taken up during travel, as ESBL-producing bacteria were detected by culture in only 1 of 35 individuals before travel while 12 of 18 students carried E. coli with the CTX-M-15 gene after their return from the Indian peninsula.…”

Section: Discussionmentioning

confidence: 99%

The Human Gut Microbiome as a Transporter of Antibiotic Resistance Genes between Continents

Bengtsson‐Palme

Angelin

Huss

et al. 2015

Antimicrob Agents Chemother

179

109

View full text Add to dashboard Cite

e Previous studies of antibiotic resistance dissemination by travel have, by targeting only a select number of cultivable bacterial species, omitted most of the human microbiome. Here, we used explorative shotgun metagenomic sequencing to address the abundance of >300 antibiotic resistance genes in fecal specimens from 35 Swedish students taken before and after exchange programs on the Indian peninsula or in Central Africa. All specimens were additionally cultured for extended-spectrum beta-lactamase (ESBL)-producing enterobacteria, and the isolates obtained were genome sequenced. The overall taxonomic diversity and composition of the gut microbiome remained stable before and after travel, but there was an increasing abundance of Proteobacteria in 25/35 students. The relative abundance of antibiotic resistance genes increased, most prominently for genes encoding resistance to sulfonamide (2.6-fold increase), trimethoprim (7.7-fold), and beta-lactams (2.6-fold). Importantly, the increase observed occurred without any antibiotic intake. Of 18 students visiting the Indian peninsula, 12 acquired ESBL-producing Escherichia coli, while none returning from Africa were positive. Despite deep sequencing efforts, the sensitivity of metagenomics was not sufficient to detect acquisition of the low-abundant genes responsible for the observed ESBL phenotype. In conclusion, metagenomic sequencing of the intestinal microbiome of Swedish students returning from exchange programs in Central Africa or the Indian peninsula showed increased abundance of genes encoding resistance to widely used antibiotics.

show abstract

“…The most obvious source is the exportation of hospital-derived strains either by colonized patients who have been discharged or by hospital workers; such events may be responsible for the introduction of the pathogenic ST131 clone carrying bla CTX-M-15 which causes significant morbidity both in hospitals and in the community setting [48]. The source for colonization with isolates carrying bla SHV may be horizontal gene transfer from exported K. pneumoniae isolates [49,50,51], while various potential environmental sources should also be considered for bla CTX-M genes other than bla CTX-M-15 , including food [52,53], companion animals [54,55,] wild animals [56,57] surface water [58], drinking water [59] and food [52], either through direct transfer of the resistant bacteria or by the horizontal spread of resistance plasmids to human commensal E. coli .…”

Section: Discussionmentioning

confidence: 99%

Carriage Rates and Characteristics of<b> </b>Enterobacteriaceae Producing Extended-Spectrum Beta-Lactamases in Healthy Individuals: Comparison of Applicants for Long-Term Care and Individuals Screened for Employment Purposes

Ebrahimi

Mózes²,

Mészáros³

et al. 2014

Chemotherapy

View full text Add to dashboard Cite

Aims: We compared the prevalence of extended-spectrum beta-lactamase (ESBL) producers in the faecal samples of 1,109 healthy individuals screened for employment purposes and in 531 asymptomatic individuals applying for long-term care (LTC). Methods: Eosin-methylene blue agar plates supplemented with 2 mg/l cefotaxime were used to determine which individuals were ESBL producers. ESBL phenotype was confirmed by double-disk synergy test and ESBL genes were identified by sequencing. ESBL producers were characterized by co-resistance and integron carriage. Results: ESBL producers were more frequent in the LTC applicants than in the employment screening individuals (7.2 vs. 2.0%; p < 0.0001), with 43 Escherichia coli, 18 Klebsiella pneumoniae, 1 Klebsiella oxytoca and 1 Proteus mirabilis being found. In the employment screening individuals, only E. coli was found. Most ESBL genes (79.4%, 50/63) were bla_CTX-M type; bla_CTX-M-15 was more frequent in the LTC applicants (p < 0.001). Regarding ESBL genes and integron diversity, E. coli isolates from the LTC applicants were more similar to K. pneumoniae than to E. coli from the employment screening individuals. Conclusion: These differences in the characteristics of ESBL producers may represent different sources of colonization. Most LTC applicants harboured K. pneumoniae or E. coli that were probably hospital-acquired whereas the E. coli isolates of many healthy individuals showed similarities to environmental E. coli.

show abstract

ESBL-Positive Enterobacteria Isolates in Drinking Water

Cited by 49 publications

References 9 publications

Prevalence of CTX-M beta-Lactamases in Escherichia coli from community-acquired urinary tract infections and associated risk factors among women in Cameroon

Prevalence of CTX-M beta-Lactamases in Escherichia coli from community-acquired urinary tract infections and associated risk factors among women in Cameroon

The Human Gut Microbiome as a Transporter of Antibiotic Resistance Genes between Continents

Carriage Rates and Characteristics of<b> </b>Enterobacteriaceae Producing Extended-Spectrum Beta-Lactamases in Healthy Individuals: Comparison of Applicants for Long-Term Care and Individuals Screened for Employment Purposes

Contact Info

Product

Resources

About