ESBL-producing bacteria and MRSA isolated from poultry and turkey products imported from Italy

Beninati, Chiara; Reich, Felix; Muscolino, Daniele; Giarratana, Filippo; Panebianco, A.; Klein, Günter; Atanassova, Viktoria

doi:10.17221/428/2014-cjfs

Cited by 25 publications

(18 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For instance, tetracycline resistance differences were not significant ( p = 0.17) between the three meat origins, consistent with the highly extended use of this antimicrobial in European livestock [ 34 ]. Moreover high resistance to tetracycline (83.0%) were observed among food isolates strains coinciding with the study performed by Beninati et al [ 35 ], who detected 85.0% of resistant strains against this antimicrobial.…”

Section: Resultssupporting

confidence: 87%

Clonal Diversity of ESBL-Producing Escherichia coli Isolated from Environmental, Human and Food Samples

Ojer-Usoz

González

Vitas

2017

IJERPH

View full text Add to dashboard Cite

This study presents a comprehensive approach of a clonal diversity analysis of 448 Extended-spectrum β-lactamase (ESBL)-producing E. coli isolated from environmental, human and food samples in Spain. The phenotypic confirmation of ESBL production was performed by disc diffusion and microdilution methods, while Polymerase Chain Reaction (PCR) and sequencing were used for the molecular characterization of β-lactamase genes (blaCTX-M, blaSHV, blaTEM, blaOXA). Clonal relationship of isolates was determined by multi-locus sequence typing (MLST). Multidrug resistant strains were present in all the studied niches, with percentages above 50.0%. The most prevalent β-lactamase genes were blaCTXM-14 (26%) and blaCTXM-1 (21.4%), followed by blaSHV-12, blaCTX-M-15 and blaTEM-42. MLST isolates were grouped into 26 clonal complexes (CC) and 177 different sequence types (ST) were detected. Despite the high clonal diversity observed, CC10 was the prevalent and the only CC detected in all niches, while other complexes as CC131 were mainly associated to human isolates. The observed prevalence and diversity of these resistant bacteria across the different environments encourages a One Health approach to prevent and control ESBL dissemination between environment and consumers.

show abstract

Section: Resultssupporting

confidence: 87%

Clonal Diversity of ESBL-Producing Escherichia coli Isolated from Environmental, Human and Food Samples

Ojer-Usoz

González

Vitas

2017

IJERPH

View full text Add to dashboard Cite

show abstract

“…This finding is worrisome as zoonotic transmission is possible and its association with human colonization and infection might pose treatment challenges to human health. The transmission and persistence of ESBL-producing bacteria through the food chain and environment through sewage contamination has been documented previously (Beninati et al, 2015; van Hoek et al, 2015). The findings of this study suggest the possibility of the transmission of both ESBL-producing clones and plasmids between humans and wild fish through environmental contamination and indicate that anthropogenic activities and the food chain as potential factors for the persistence and dissemination of bla CTX-M-15 in Mwanza City.…”

Section: Discussionmentioning

confidence: 70%

Predominance of CTX-M-15 among ESBL Producers from Environment and Fish Gut from the Shores of Lake Victoria in Mwanza, Tanzania

et al. 2016

View full text Add to dashboard Cite

Extended-Spectrum Beta-Lactamase (ESBL)-producing bacteria are a common cause of healthcare and community-associated infections worldwide. The distribution of such isolates in the environment and their presence in fish as a result of sewage contamination is not well-studied. Here we examined fish and environmental samples from Mwanza city for the presence of ESBL-producing bacteria. From 196 fish sampled from local markets, 26 (13.3%) contained lactose-fermenting ESBL-producing bacteria, while 39/73 (53.4%) environmental samples from the same area were ESBL producers. Antibiotic resistance genes, multi locus sequence types (MLST) and plasmid replicon types in 24 selected isolates from both populations were identified with whole genome sequencing using Illumina MiSeq. Nine of eleven sequenced fish isolates had the blaCTX-M-15 gene whereas 12/13 from environment carried blaCTX-M-15. Antibiotic resistance genes encoding resistance to sulfonamides (sul1/sul2), tetracyclines [tet(A)/tet(B)] fluoroquinolones [e.g., aac(6′)-Ib-cr, qnrS1], aminoglycosides [e.g., aac(3)-lld, strB, strA,] and trimethoprim (e.g., dfrA14) were detected. E. coli sequence type ST-38 (2) and ST-5173 (2) were detected in isolates both from the environment and fish. IncY plasmids carrying blaCTX-M-15, qnrS1, strA, and strB were detected in five environmental E. coli isolates and in one E. coli isolate from fish. Our data indicate spillage of resistant environmental isolates into Lake Victoria through the sewage system. Persistence of blaCTX-M-15 in the Mwanza city environment is complex, and involves both clonal spread of resistant strains as well as dissemination by commonly occurring IncY plasmids circulating in isolates present in humans, the environment as well as in the food chain.

show abstract

“…After assessing all the information from the recovered publications, seventy-eight primary studies [6,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80,81,82,…”

Section: Methodsmentioning

confidence: 99%

Prevalence of Pathogens in Poultry Meat: A Meta-Analysis of European Published Surveys

Gonçalves-Tenório

Silva

Rodrigues

et al. 2018

Foods

116

View full text Add to dashboard Cite

The objective of this study was to investigate and summarize the levels of incidence of Salmonella spp., Listeria monocytogenes, Staphylococcus aureus and Campylobacter spp. in poultry meat commercialized in Europe. After systematic review, incidence data and study characteristics were extracted from 78 studies conducted in 21 European countries. Pooled prevalence values from 203 extracted observations were estimated from random-effects meta-analysis models adjusted by pathogen, poultry type, sampling stage, cold preservation type, meat cutting type and packaging status. The results suggest that S. aureus is the main pathogen detected in poultry meat (38.5%; 95% CI: 25.4–53.4), followed by Campylobacter spp. (33.3%; 95% CI: 22.3–46.4%), while L. monocytogenes and Salmonella spp. present lower prevalence (19.3%; 95% CI: 14.4–25.3% and 7.10%; 95% CI: 4.60–10.8%, respectively). Despite the differences in prevalence, all pathogens were found in chicken and other poultry meats, at both end-processing step and retail level, in packed and unpacked products and in several meat cutting types. Prevalence data on cold preservation products also revealed that chilling and freezing can reduce the proliferation of pathogens but might not be able to inactivate them. The results of this meta-analysis highlight that further risk management strategies are needed to reduce pathogen incidence in poultry meat throughout the entire food chain across Europe, in particular for S. aureus and Campylobacter spp.

show abstract

ESBL-producing bacteria and MRSA isolated from poultry and turkey products imported from Italy

Cited by 25 publications

References 23 publications

Clonal Diversity of ESBL-Producing Escherichia coli Isolated from Environmental, Human and Food Samples

Clonal Diversity of ESBL-Producing Escherichia coli Isolated from Environmental, Human and Food Samples

Predominance of CTX-M-15 among ESBL Producers from Environment and Fish Gut from the Shores of Lake Victoria in Mwanza, Tanzania

Prevalence of Pathogens in Poultry Meat: A Meta-Analysis of European Published Surveys

Contact Info

Product

Resources

About