As a result of the COVID-19 pandemic, more and more organizations have implemented remote working, resulting in a partial overlap between home and work environments. This study aimed to develop a tool named Perceived Remote Workplace Environment Quality Indicators (PRWEQIs) to study the impact of the remote work environment on worker well-being. A preliminary 20-item version was developed. In Study 1, an Exploratory Factor Analysis (EFA) was conducted on a sample of remote students (N = 521); the results were confirmed in Study 2 through a Confirmatory Factor Analysis (CFA) on a sample of remote workers (N = 463). The final 15-item PRWEQIs include five indicators, Acoustic comfort, Visual comfort, Quality of the furnishings, Safety, and Space usability, and a second-order factor referring to General perceived comfort. The scale constitutes an initial instrument for assessing the perception of the physical-spatial qualities of the remote working environment.