Escapist theatre in wartime

Thermal inactivation of staphylococcal enterotoxin B with changing pH, protein concentration, and ionic strength

“…The v.'her; -njected întran^ritnnpally Into kittens did not give a positive reaction. Neutralization was also demonstrated by Dolman (1944) fay intravenous injection of enterotoxinserum mixtures into cats and by Surgalla et (1954) through monkey feeding tests» In 1958, Gasman was able to give evidence that the entero toxin can confer passive immunity. Serum from immunized rabbits protected cats when injected with this serum 3 to 5 minutes before injection of the challenging enterotoxin» Serological studies made by Bergdol1 et (1959b) and Gasman (1958, i960) for the purpose of devising an assay method for the enterotoxin re vealed the presence of more than one enterotoxin» Using antiserum to the known food poisoning strains I9&E, 243 and S-6 and a modification of Ouchterlony's agar diffusion method (1953), Gasman found that 19&E pro duced an antigen common to 13 (including S-6 but not 243) of 22 supposedly enterotoxigenic strains whereas strain 243 produced an antibody which re acts only with enterotoxin from 243 and S-6» The I96E type antigen was tentatively named enterotoxin F (food poisoning) and the 243 type antigen, enterotoxin E (enteritis) (Gasman,I96O).…”

“…Reports were also conflicting as to its antigenicity (Dack £t £l^., 1931 ;Wool pert and Deck, 1933 ;Minett, 1938;Wilson, 1938, 1940;Harmon, 1941;Dolman, 1944). Some measure of increased tolerance to the enterotoxin had been demonstrated, and Dolman and Wilson (1938) reported a specific flocculation reaction between the enterotoxin and its homologous antibody but no conclusive evidence for passive Immunity had been shown.…”