Escherichia coli and Salmonella spp. in meat in Jeddah, Saudi Arabia

Iyer, Archana; Kumosani, Taha; Yaghmoor, Soonham; Barbour, Elie K.; Azhar, Esam I.; Harakeh, Steve

doi:10.3855/jidc.3453

Cited by 18 publications

(11 citation statements)

References 20 publications

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“…Observations of the current state of animal slaughter and processing in Saudi Arabia suggests that hide to carcass transfer is occurring and no antimicrobial intervention measures to reduce the level of pathogens on carcasses as used in the United States are in place. This is supported by a recent report by Iyer et al (20) of E. coli and Salmonella isolated from nonspecified meats collected from market places in Jeddah, KSA.…”

Section: 05)supporting

confidence: 76%

Prevalence of Escherichia coli O157:H7 and Salmonella in Camels, Cattle, Goats, and Sheep Harvested for Meat in Riyadh

Bosilevac

Gassem

Sheddy

et al. 2015

Journal of Food Protection

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Escherichia coli O157:H7 and Salmonella are significant foodborne pathogens that can be found in the feces and on the hides of meat animals. When hides are removed during the harvest process, the carcass and subsequent meat products can become contaminated. Camels, cattle, sheep, and goats are harvested for meat in Riyadh, Saudi Arabia. The prevalence of E. coli O157:H7 and Salmonella are unknown in these animals, and it is assumed that if the animals carry the pathogens in their feces or on their hides, meat products are likely to become contaminated. To this end, a minimum of 206 samples each from hides and feces of camels, cattle, goats, and sheep were collected over the course of 8 months and tested for E. coli O157:H7 and Salmonella. It was found that E. coli O157:H7 was present in feces (10.7, 1.4, 2.4, and 2.4%) and on hides (17.9, 8.2, 2.9, and 9.2%) of cattle, goats, camels, and sheep, respectively. The prevalence of Salmonella was 11.2, 13.5, 23.2, and 18.8% in feces and 80.2, 51.2 67.6, and 60.2% on hides of cattle, goats, camels, and sheep, respectively. The prevalence of E coli O157:H7 was nearly zero in all samples collected in June and July, while Salmonella did not exhibit any seasonal variation. These results constitute the first comprehensive study of E. coli O157:H7 and Salmonella prevalence in Saudi Arabian meat animals at harvest.

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Section: 05)supporting

confidence: 76%

Prevalence of Escherichia coli O157:H7 and Salmonella in Camels, Cattle, Goats, and Sheep Harvested for Meat in Riyadh

Bosilevac

Gassem

Sheddy

et al. 2015

Journal of Food Protection

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“…Salmonellosis is a widespread foodborne zoonosis in many countries (Wegener and Baggesen, 1996;Lo Fo Wong, 2002;Iyer et al, 2013); people of all ages are affected, and the incidence is highest in children. In the developing world, human immunodeficiency virus infection and AIDS are important risk factors for nontyphoidal salmonellosis and bacteremia (Obi and Bessong, 2002); and several studies have shown in infected African adults with documented bloodstream infections that nontyphoidal salmonellae were isolated from up to 35% of adults (Hohmann, 2001).…”

Section: Introductionmentioning

confidence: 99%

SalmonellaandCampylobacterContamination of Ready-to-Eat Street-Vended Pork Meat Dishes in Antananarivo, Madagascar: A Risk for the Consumers?

CardinaleEric

AbatCédric²,

BénédicteContamin³

et al. 2015

Foodborne Pathogens and Disease

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Street-food vending has been increasing in many developing countries and particularly in Madagascar since 2000. Gastroenteric diseases cause 37% of all deaths each year, and 50% of children < 5 years are infected with intestinal pathogens. However, there has been little information regarding the incidence of street-food-related diseases, or foodborne pathogens in pork, which is the most commonly eaten meat, along with chicken. Thus, the aim of this study was to investigate the safety of traditional ready-to-eat street-vended pork dishes and to assess the association of restaurant characteristics and cooking practices with Salmonella and Campylobacter contamination of these meals. Sixty street-restaurants were studied from March 2012 to August 2012 in Antananarivo. A questionnaire was submitted to the managers, and samples of ready-to-eat pork dishes were bought. Salmonella spp. were isolated in 10% of the 60 street-restaurants studied and in 5% samples of pork dishes. The most prevalent serovars isolated were Salmonella Typhimurium (44%) and Senftenberg (33%). Campylobacter was not detected. Only 4 of the 43 variables tested in the screening analysis were significantly associated with Salmonella spp. contamination of the street-restaurants. The risk for a restaurant to be Salmonella positive decreased when there were specific premises for the restaurant and when the staff was wearing specific clothes when working. Conversely, that risk increased when the temperature of ready-to-eat pork was < 52°C and when tablecloths were used in the restaurant.

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“…The conventional microbiological methods for detection of these bacteria, however, usually include multiple subcultures and biotype or serotype identification steps and, thus are laborious and time-consuming [ 9 ]. With the development of molecular techniques, polymerase chain reaction (PCR) has become an important tool for detecting pathogenic microorganisms in food products which improved the sensitivity, specificity, and speed of detection [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus, and Salmonella enterica Typhimurium in meat and meat products using multiplex polymerase chain reaction

Latha¹,

Anu²,

Ajaykumar³

et al. 2017

Vet World

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Aim:The objective of the study was to investigate the occurrence of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus, and Salmonella enterica Typhimurium in meat and meat products using the multiplex polymerase chain reaction (PCR) method.Materials and Methods:The assay combined an enrichment step in tryptic soy broth with yeast extract formulated for the simultaneous growth of target pathogens, DNA isolation and multiplex PCR. A total of 1134 samples including beef (n=349), chicken (n=325), pork (n=310), chevon (n=50), and meat products (n=100) were collected from different parts of Kerala, India. All the samples were subjected to multiplex PCR analysis and culture-based detection for the four pathogens in parallel.Results:Overall occurrence of L. monocytogenes was 0.08 % by cultural method. However, no L. monocytogenes was obtained by multiplex PCR method. Yersinia enterocolitica was obtained from beef and pork samples. A high prevalence of S. aureus (46.7%) was found in all types of meat samples tested. None of the samples was positive for S. Typhimurium.Conclusion:Multiplex PCR assay used in this study can detect more than one pathogen simultaneously by amplifying more than one target gene in a single reaction, which can save time and labor cost.

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Escherichia coli and Salmonella spp. in meat in Jeddah, Saudi Arabia

Cited by 18 publications

References 20 publications

Prevalence of Escherichia coli O157:H7 and Salmonella in Camels, Cattle, Goats, and Sheep Harvested for Meat in Riyadh

Prevalence of Escherichia coli O157:H7 and Salmonella in Camels, Cattle, Goats, and Sheep Harvested for Meat in Riyadh

SalmonellaandCampylobacterContamination of Ready-to-Eat Street-Vended Pork Meat Dishes in Antananarivo, Madagascar: A Risk for the Consumers?

Prevalence of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus, and Salmonella enterica Typhimurium in meat and meat products using multiplex polymerase chain reaction

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