Escherichia coli expression and purification of LL37 fused to a family III carbohydrate-binding module from Clostridium thermocellum

Ramos, Reinaldo Rodrigues; Domingues, Lucı́lia; Gama, Miguel

doi:10.1016/j.pep.2009.10.016

Cited by 40 publications

(35 citation statements)

References 24 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the molecular mechanisms involved in LL37 improvement of healing are not clear. Recently, a recombinant version of this peptide that was able to maintain its antimicrobial activity was produced using a cost-effective method [13]. This recombinant LL37 was shown to accelerate the dexamethasone-induced impaired healing of excisional wounds in C57BL/6 mice [14], further suggesting its potential use as an inexpensive therapeutic agent.…”

Section: Introductionmentioning

confidence: 99%

Improved burn wound healing by the antimicrobial peptide LLKKK18 released from conjugates with dextrin embedded in a carbopol gel

et al. 2015

Self Cite

View full text Add to dashboard Cite

This work presents a newly developed formulation that holds great potential as a therapeutic approach for burn treatment. It is based on the sustained delivery of an antimicrobial peptide - LLKKK18 - from conjugates with dextrin, after degradation of dextrin backbone upon exposure to wound α-amylases. Conjugates were further embedded in Carbopol®, a commercially available hydrogel, suitable for topical administration and that provides a moist environment to the wound. Overall, we obtained an efficient, safe and non-expensive formulation that improves burn wound healing, maintains a moist environment within the wound, is easy to apply-and-remove, and has potential to prevent infection due to the presence of an antimicrobial peptide. Importantly, this is the first time the wound healing ability of LLKKK18 is demonstrated and that its main mechanisms of action are identified.

show abstract

Section: Introductionmentioning

confidence: 99%

Improved burn wound healing by the antimicrobial peptide LLKKK18 released from conjugates with dextrin embedded in a carbopol gel

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Another application of engineered CBM is to characterize native and engineered carbohydrate biomaterials by using various fluorescent techniques (Gourlay et al 2012;Gao et al 2014). Apart from this, CBMs can serve as attractive affinity tags and, hence, used in the production and purification of antibodies, peptides and enzymes (Hyeon et al 2011(Hyeon et al , 2012Ramos et al 2010;Wan et al 2011). Ramos et al (2013) have reported the production and purification of recombinant antimicrobial peptides in Escherichia coli by using CBM.…”

Section: Applications Of Cellulosomesmentioning

confidence: 99%

Bioprospecting thermostable cellulosomes for efficient biofuel production from lignocellulosic biomass

Arora

Behera

Sharma

et al. 2015

Bioresour. Bioprocess.

View full text Add to dashboard Cite

The adverse climatic conditions due to continuous use of fossil-derived fuels are the driving factors for the development of renewable sources of energy. Current biofuel research focuses mainly on lignocellulosic biomass (LCB) such as agricultural, industrial and municipal solid wastes due to their abundance and renewability. Although many mesophilic cellulolytic microorganisms have been reported, efficient and economical bioconversion to simple sugars is still a challenge. Thermostable cellulolytic enzymes play an indispensible role in degradation of the complex polymeric structure of LCB into fermentable sugar stream due to their higher flexibility with respect to process configurations and better specific activity than the mesophilic enzymes. In some anaerobic thermophilic/thermotolerant microorganisms, few cellulases are organized as unique multifunctional enzyme complex, called the cellulosome. The use of cellulosomal multienzyme complexes for saccharification seems to be a promising and cost-effective alternative for complete breakdown of cellulosic biomass. This paper aims to explore and review the important findings in cellulosomics and forward the path for new cutting-edge opportunities in the success of biorefineries. Herein, we summarize the protein structure, regulatory mechanisms and their expression in the host cells. Furthermore, we discuss the recent advances in specific strategies used to design new multifunctional cellulosomal enzymes, which can function as lignocellulosic biocatalysts and evaluate the roadblocks in the yield and stability of such designer thermozymes with overall progress in lignocellulose-based biorefinery.

show abstract

“…This scalable method can purify the CBM-tag protein by using a simple unit operation, involving centrifugation or filtration. For example, the cathelicidin-derived human peptide LL37, which has a broad spectrum of antimicrobial and immunomodulatory activities, was cloned to the N-and C-termini of a CBM3 fused to the linker sequence (LK-CBM3) from Clostridium thermocellum (Ramos et al 2010). In the course of processing, the purification was achieved using cellulose CF11 fibers, taking advantage of the CBM3's specific affinity for cellulose.…”

Section: Applications Of Proteins With Fused Cbds Fusion Of One or Momentioning

confidence: 99%

A Mini-review on the Applications of Cellulose-Binding Domains in Lignocellulosic Material Utilizations

Yang¹,

Jin²,

Zhu³

et al. 2015

BioResources

View full text Add to dashboard Cite

This manuscript provides a mini review on the fundamentals of cellulose binding domains (CBDs) or cellulose binding modules (CBMs) and their applications using lignocellulosic materials. CBDs, the non-productive part of cellulases, have miscellaneous biological functions and have been widely applied in lignocellulose hydrolysis, protein engineering, structural support, metabolism, energy storage, antibiosis, immunological recognition, targeting, attachment, etc. due to their specific affinity to various substrates of lignocelluloses. Understanding of the properties and mechanisms of CBDs is of vital significance because it provides the basis for fine manipulation of cellulose-CBM interactions and eventually improves the bioconversion performance of lignocelluloses into fuels and desired chemicals. In this short review, the fundamentals of CBD, the definition of CBM family, and the structures of different CBM families are introduced. Then recent findings in the applications of CBDs are discussed relative to the lignocelllulosic utilizations.

show abstract

Escherichia coli expression and purification of LL37 fused to a family III carbohydrate-binding module from Clostridium thermocellum

Cited by 40 publications

References 24 publications

Improved burn wound healing by the antimicrobial peptide LLKKK18 released from conjugates with dextrin embedded in a carbopol gel

Improved burn wound healing by the antimicrobial peptide LLKKK18 released from conjugates with dextrin embedded in a carbopol gel

Bioprospecting thermostable cellulosomes for efficient biofuel production from lignocellulosic biomass

A Mini-review on the Applications of Cellulose-Binding Domains in Lignocellulosic Material Utilizations

Contact Info

Product

Resources

About