Escherichia coli O157:H7: distribution, molecular characterization, antimicrobial resistance patterns and source of contamination of sheep and goat carcasses at an export abattoir, Mojdo, Ethiopia

Abreham, Solomon; Teklu, Akafete; Cox, Eric; Tessema, Tesfaye Sisay

doi:10.1186/s12866-019-1590-8

Cited by 35 publications

(19 citation statements)

References 42 publications

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“…Most the E. coli O157:H7 strains, however, produce stx2, although either stx1, or stx2, or both are produced (20). The results of this study showed that the percentage of the stx2 gene 44.4% is higher than the ratio of the stx1gene 33.3% of all isolates for dogs with and without diarrhea, This results disagree with study in Iran by Torkan et al (21) which appeared the stx1 gene 64.3% was higher than stx2 gene 35.7% in E. coli O157H:7 isolates from feces of dogs suffering from diarrhea (21).while our results were agreement with study on sheep by Abreham et al (22) showed the percentage of the stx2 gene 57.1% higher than stx1 gene 14.2% (22).…”

Section: Discussioncontrasting

confidence: 88%

Molecular detection of rfbO157, shiga toxins and hemolysin genes for Escherichia coli O157: H7 from canine feces in Tikrit and Mosul cities, Iraq

Abdulrazzaq¹,

Owain²,

Majeed³

et al. 2021

IJVS

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Escherichia coli O157:H7 is considered as an important pathogen of diarrhea in adult dogs and puppies because it contains virulence genes. The study objective was to the molecular detection of the rfbO157 encoding the O-antigen specific for E. coli O157: H7,shiga toxins and hemolysin genes of E.coli O157:H7 in feces of dogs that collected from different regions in Tikrit and Mosul cities, Iraq. One hundred fecal swabs were collected from pet and K9 dogs including (72 dogs with diarrhea, and 28 without diarrhea). All the Collected swabs were cultured in the nutrient and MacConkey agars, Then the suspected colonies were cultured in the EMB agar. Metallic sheen colonies were cultured by using the chrome agar. All bacteriological identified isolates were enrolled by using the polymerase chain reaction (PCR) technique. The results of this study showed that 7(9.7%) of 72 dogs suffered from diarrhea were positive for E. coli O157:H7 that contained the rfbO157 gene (n= 6), carry stx1 gene (n= 3), carry stx2 gene (n= 3), and hlyA gene (n= 1). On the other hand, 2 (7.1%) of 28 dogs without diarrhea were positive for E. coli O157:H7 that contained the rfbO157 gene (n= 1), stx2 gene (n= 1), and hlyA gene (n= 1). In conclusion, dogs can be a significant reservoir for pathogenic E. coli O157:H7, particularly dogs with diarrhea.

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Section: Discussioncontrasting

confidence: 88%

Molecular detection of rfbO157, shiga toxins and hemolysin genes for Escherichia coli O157: H7 from canine feces in Tikrit and Mosul cities, Iraq

Abdulrazzaq¹,

Owain²,

Majeed³

et al. 2021

IJVS

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“…Regarding the prevalence of E. coli O157: H7 in association with risk factors such as age, sex and body condition scores, during the present study it was found that there was significant variation among different slaughtered cattle age groups (young 16.7%, adult 7.4% and old 8.7%) of the study cattle with respect to the prevalence of E. coli O157: H7 (p<0.05). This finding is inline with the findings of Abreham et al 28 who reported the prevalence of E. coli O157: H7 can significantly vary in relation to the age of the cattle. However, the finding by Mekonnen et al 35 showed insignificant statistical difference (p> 0.05) between the age groups regarding the prevalence of E. coli O157: H7 which contradicts the present finding which could be related to the underdeveloped immune status of young cattle.…”

Section: Discussionsupporting

confidence: 92%

“…This considerable variation could be attributed to the prolonged storage of the isolated E. coli samples with glycerol suspension at low temperature. The sample type based prevalence of E. coli O157: H7 from feaces also is in agreement with the findings from these studies 28 , 29 from Hawassa and Canada respectively.…”

Section: Discussionsupporting

confidence: 89%

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Antimicrobial Profiles and Conventional PCR Assay of Shiga Toxigenic Escherichia coli O157:H7 (STEC) Isolated from Cattle Slaughtered at Bedele Municipal Abattoir, South West Ethiopia

Fikadu

Kabeta

Diba

et al. 2023

IDR

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Background Shiga toxin producing Escherichia coli O157:H7 (STEC) is considered the most prevalent food borne pathogen that has gained increasing attention worldwide in recent years. Methods A cross-sectional study was carried out at Bedele Municipal abattoir on cattle that were reported healthy from detailed ante-mortem inspections and having various body conditions scores. A total of 516 samples were collected and examined after enriched in modified peptone water. Following an enrichment, the samples were plated onto MacConkey agar and then onto Eosin methylene blue agar. Finally after a few similar procedures, 14 E. coli O157:H7 (STEC) isolates were confirmed through latex agglutination test. The collected data were analyzed using SPSS version 20 statistical software. Results This study finding revealed that the overall prevalence of E. coli O157:H7 out of 516 samples was found to be 2.7%. However, on sample type basis, the prevalence of E. coli O157:H7 from feacal samples, carcass swabs, butcher hand swabs and knife swabs were 4.7%, 3.3%, 1.1% and 1.1%, respectively. It was also found that that the prevalence of E. coli O157:H7 was significantly affected by age groups of slaughtered cattle (p<0.05). Moreover, in vitro antimicrobial susceptibility test result on average showed that almost all of E. coli O157:H7 isolates were highly susceptible to kanamycin and no resistance was shown to ciprofloxacin and gentamicin. Finally, the conventional PCR detection of stx1, st2 and hylA genes revealed that only 21.4% and 14.3% were found to contain stx1 and hylA genes respectively. Conclusion To wrap up, this study showed that Shiga toxin producing E. coli O157:H7 (STEC) isolates were found with almost low overall prevalence rate from all sample sources in this study site. Therefore, improving abattoir facilities and slaughter house workers’ personal hygiene are recommended to curtail E. coli O157:H7 meat contamination in this abattoir.

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“…Escherichia coli O157:H7 is one of the most ubiquitous foodborne zoonotic pathogens [1][2][3]. Sewage irrigation and manure application to soil have caused the pathogen to seep into soil matrices [4].…”

Section: Introductionmentioning

confidence: 99%

The attachment process and physiological properties of Escherichia coli O157:H7 on quartz

Wang

Cai

et al. 2020

BMC Microbiol

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Background Manure application and sewage irrigation release many intestinal pathogens into the soil. After being introduced into the soil matrix, pathogens are commonly found to attach to soil minerals. Although the survival of mineral-associated Escherichia coli O157:H7 has been studied, a comprehensive understanding of the attachment process and physiological properties after attachment is still lacking. Results In this study, planktonic and attached Escherichia coli O157:H7 cells on quartz were investigated using RNA sequencing (RNA-seq) and the isobaric tagging for relative and absolute quantitation (iTRAQ) proteomic method. Based on the transcriptomic and proteomic analyses and gene knockouts, functional two-component system pathways were required for efficient attachment; chemotaxis and the Rcs system were identified to play determinant roles in E. coli O157:H7 attachment on quartz. After attachment, the pyruvate catabolic pathway shifted from the tricarboxylic acid (TCA) cycle toward the fermentative route. The survival rate of attached E. coli O157:H7 increased more than 10-fold under penicillin and vancomycin stress and doubled under alkaline pH and ferric iron stress. Conclusions These results contribute to the understanding of the roles of chemotaxis and the Rcs system in the attachment process of pathogens and indicate that the attachment of pathogens to minerals significantly elevates their resistance to antibiotics and environmental stress, which may pose a potential threat to public health.

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Escherichia coli O157:H7: distribution, molecular characterization, antimicrobial resistance patterns and source of contamination of sheep and goat carcasses at an export abattoir, Mojdo, Ethiopia

Cited by 35 publications

References 42 publications

Molecular detection of rfbO157, shiga toxins and hemolysin genes for Escherichia coli O157: H7 from canine feces in Tikrit and Mosul cities, Iraq

Molecular detection of rfbO157, shiga toxins and hemolysin genes for Escherichia coli O157: H7 from canine feces in Tikrit and Mosul cities, Iraq

Antimicrobial Profiles and Conventional PCR Assay of Shiga Toxigenic Escherichia coli O157:H7 (STEC) Isolated from Cattle Slaughtered at Bedele Municipal Abattoir, South West Ethiopia

The attachment process and physiological properties of Escherichia coli O157:H7 on quartz

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