Escherichia Coli Redox Enzyme Maturation Proteins, TorD and DmsD Interact with GTP as Shown by Native Page Assays

Abstract

The twin-arginine translocation (Tat) system can transport fully folded proteins across the cytoplasmic membrane. Transport is dependent on a twin-arginine motif within a cleavable signal peptide. Substrates for the Tat system include redox proteins, necessary for growth during anaerobic respiration. The transport of the catalytic subunits responsible for N-and S-oxide respiration, DmsAB and TorA are dependent on DmsD and TorD respectively. Both are