Escherichia coli sequence type 73 as a cause of community acquired urinary tract infection in men and women in Rio de Janeiro, Brazil

da-Silva, Ana Paula de Souza; Sousa, Viviane Santos de; Martins, Natacha; Dias, Rubens Clayton da Silva; Bonelli, Raquel Regina; Riley, Lee W.; Moreira, Beatriz Meurer

doi:10.1016/j.diagmicrobio.2017.01.024

Cited by 16 publications

(10 citation statements)

References 30 publications

(44 reference statements)

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“…The traT gene was mostly detected among ST69 isolates. This finding has been reported in previous surveys 20. The traT protein is responsible for development of a resistance phenotype against serum bactericidal activity, and may confer additional advantage to ST69 isolates.…”

Section: Discussionsupporting

confidence: 87%

Characterization of antibiotic-susceptibility patterns and virulence genes of five major sequence types of Escherichia coli isolates cultured from extraintestinal specimens: a 1-year surveillance study from Iran

Hojabri¹,

Mirmohammadkhani²,

Darabi³

et al. 2019

IDR

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Objectives Escherichia coli sequence types (STs) 69, 73, 95, 127, and 131 are major STs frequently causing extraintestinal infections. The prevalence of specific clones and their virulence and resistance profiles has not been described from Iran. The aim of this study was to characterize antimicrobial-susceptibility profiles and virulence traits of five major clones of E. coli recovered from human extraintestinal infections in Semnan, Iran. We compared these traits between major ST clones and also between O25b and O16 subgroups of the ST131 clone. Methods We characterized the five major ST clones among 335 collected E. coli isolates obtained from extraintestinal infections, and phylogenetic groups, antimicrobial susceptibility, and virulence/resistance-gene profiles of these major STs were studied. Results The highest rates of the multidrug-resistance phenotype were detected among ST131 (85.7%) and ST69 (41.7%), and trimethoprim/sulfamethoxazole resistance was detected significantly among the latter clone. Of the 151 isolates belonging to major ST clones, bla OXA-48 was detected among all except the ST127 clone, while bla NDM genes were harbored by 14 (9.2%) isolates, which all belonged to the ST131 clone. Aggregate virulence scores (median) of ST131 isolates (11) were slightly higher than ST69 (8.50) strains, but were lower than ST73 (16), ST95 (16), and ST127 (12.50) isolates. Principal-coordinate analysis revealed distinct virulence profiles with the ST131 clone. ST73, ST95 and ST131 were enriched with “urovirulence” traits, including phylogroup B2 and group B2-associated accessory traits ( chuA , iutA , yfcV , papGII , usp , kpsMTII and malX ) and the derived variables extraintestinal pathogenic E. coli and uropathogenic E. coli . In contrast, ST69 was depleted of these traits, but enriched with phylogroups D and E. Conclusion Our data emphasize that isolates of the ST131 clone have the ability to make a balance between resistance and virulence traits to establish a wider clone in extraintestinal pathogenic E. coli .

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Section: Discussionsupporting

confidence: 87%

Characterization of antibiotic-susceptibility patterns and virulence genes of five major sequence types of Escherichia coli isolates cultured from extraintestinal specimens: a 1-year surveillance study from Iran

Hojabri¹,

Mirmohammadkhani²,

Darabi³

et al. 2019

IDR

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“…This study characterised the E. coli strains isolated from DFOM and deep DFI and demonstrated that these bacteria were highly genetically diverse with different pathogenicity traits. Among them, some strains belonged to ST73, a major clonal complex isolated from UTI particularly pyelonephritis and associated with multidrug resistance profile [35][36][37]. Moreover, these strains were isolated from monomicrobial infection in our panel confirming their virulence potential.…”

Section: Discussionsupporting

confidence: 53%

Escherichia coli Isolated from Diabetic Foot Osteomyelitis: Clonal Diversity, Resistance Profile, Virulence Potential, and Genome Adaptation

et al. 2021

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This study assessed the clonal diversity, the resistance profile and the virulence potential of Escherichia coli strains isolated from diabetic foot infection (DFI) and diabetic foot osteomyelitis (DFOM). A retrospective single-centre study was conducted on patients diagnosed with E. coli isolated from deep DFI and DFOM at Clinique du Pied Diabétique Gard-Occitanie (France) over a two-year period. Phylogenetic backgrounds, virulence factors (VFs) and antibiotic resistance profiles were determined. Whole-genome analysis of E. coli strains isolated from same patients at different periods were performed. From the two-years study period, 35 E. coli strains isolated from 33 patients were analysed; 73% were isolated from DFOM. The majority of the strains belonged to the virulent B2 and D phylogenetic groups (82%). These isolates exhibited a significant higher average of VFs number than strains belonging to other groups (p < 0.001). papG2 gene was significantly more detected in strains belonging to B2 phylogroup isolated from DFI compared to DFOM (p = 0.003). The most prevalent antibiotic resistance pattern was observed for ampicillin (82%), cotrimoxazole (45%), and ciprofloxacin (33%). The genome analysis of strains isolated at two periods in DFOM showed a decrease of the genome size, and this decrease was more important for the strain isolated at nine months (vs. four months). A shared mutation on the putative acyl-CoA dehydrogenase-encoding gene aidB was observed on both strains. E. coli isolates from DFOM were highly genetically diverse with different pathogenicity traits. Their adaptation in the bone structure could require genome reduction and some important modifications in the balance virulence/resistance of the bacteria.

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“…En 2017 Souza, et al reportaron una frecuencia del gen afa en aislamientos UPEC de 9 % (18) , pero no encontraron una asociación significativa entre el gen y el filogrupo, ni con el género del paciente de donde se aisló. Además, mostraron que la presencia del gen afa y la no sensibilidad a la amika-Figura 1.…”

Section: Discussionunclassified

Presencia de genes fimH y afa en aislamientos urinarios de Escherichia coli productora de betalactamasas de espectro extendido en Lima, Perú

Matta-Chuquisapon

Valencia-Bazalar

Marocho-Chahuayo

et al. 2020

Rev Peru Med Exp Salud Publica

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Con el objetivo de determinar la presencia de los genes fimH y afa en aislamientos urinarios de Escherichia coli productoras de betalactamasas de espectro extendido (BLEE), se realizó un estudio descriptivo, con aislamientos del cepario del proyecto TO-06/09 del Instituto Nacional de Salud del Niño en Lima, Perú. Se incluyeron 75 aislamientos urinarios de Escherichia coli. La identificación de genes se realizó por reacción en cadena de la polimerasa. De los 75 aislamientos, 74 (98,7%) fueron positivos para el gen fimH y 6 (8,0%) fueron positivos para el gen afa. Se evidenció la presencia de los factores de virulencia producidos por los genes fimH y afa en aislamientos urinarios de Escherichia coli productoras de BLEE.

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Escherichia coli sequence type 73 as a cause of community acquired urinary tract infection in men and women in Rio de Janeiro, Brazil

Cited by 16 publications

References 30 publications

<p>Characterization of antibiotic-susceptibility patterns and virulence genes of five major sequence types of <em>Escherichia coli</em> isolates cultured from extraintestinal specimens: a 1-year surveillance study from Iran</p>

<p>Characterization of antibiotic-susceptibility patterns and virulence genes of five major sequence types of <em>Escherichia coli</em> isolates cultured from extraintestinal specimens: a 1-year surveillance study from Iran</p>

Escherichia coli Isolated from Diabetic Foot Osteomyelitis: Clonal Diversity, Resistance Profile, Virulence Potential, and Genome Adaptation

Presencia de genes fimH y afa en aislamientos urinarios de Escherichia coli productora de betalactamasas de espectro extendido en Lima, Perú

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