Escherichia coli toxin gene hipA affects biofilm formation and DNA release

Zhao, Junqiao; Wang, Qian; Li, Mingji; Heijstra, Björn D.; Wang, Shengjun; Liang, Quanfeng; Qi, Qingsheng

doi:10.1099/mic.0.063784-0

Cited by 47 publications

(34 citation statements)

References 38 publications

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“…Another difference relates to a contribution of these TAs to biofilm formation. For example, Zhao et al (2013) showed that HipAB plays a significant role in biofilm development through extracellular DNA (eDNA) release, a property not associated with MazEFor DinJYafQ-mediated cell death (Kolodkin-Gal et al, 2009). Finally, the common presence of the hipAB locus in B2 and B1 strains may be associated with auxiliary regulation of 33 diverse genes, including those associated with metabolism, transportation, transcriptional regulation and mismatch repair (Lin et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Type II toxin–antitoxin systems are unevenly distributed among Escherichia coli phylogroups

et al. 2015

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Type II toxin-antitoxin systems (TAs) are bicistronic operons ubiquitous in prokaryotic genomes, displaying multilevel association with cell physiology. Various possible functions have been assigned to TAs, ranging from beneficial for their hosts, such as a stress response, dormancy and protection against genomic parasites, to detrimental or useless functions, such as selfish alleles. As there is a link between several Escherichia coli features (e.g. virulence, lifestyle) and the phylogeny of this species, we hypothesized a similar association with TAs. Using PCR we studied the distribution of 15 chromosomal and plasmidic type II TA loci in 84 clinical E. coli isolates in relation to their main phylogenetic groups (A, B1, B2 and D). In addition, we performed in silico searching of these TA loci in 60 completely sequenced E. coli genomes deposited in GenBank. The highest number of TA loci per strain was observed in group A (mean 8.2, range 5-12) and the lowest in group B2 (mean 4.2, range 2-8). Moreover, significant differences in the prevalence of nine chromosomal TAs among E. coli phylogroups were noted. In conclusion, the presence of some chromosomal TAs in E. coli is phylogroup-related rather than a universal feature of the species. In addition, their limited collection in group B2 clearly distinguish it from the other E. coli phylogroups.

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Section: Discussionmentioning

confidence: 99%

Type II toxin–antitoxin systems are unevenly distributed among Escherichia coli phylogroups

et al. 2015

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“…The inhibition of CsgD causes decreases in the signalling nucleotide c-di-GMP and curli production, which subsequently reduces biofilm formation. In addition, HipA releases extracellular DNAs that contribute to biofilm adhesion [39], and the TA modules of E. coli , YefM–YoeB, DinJ–YafQ, and RelBE, are related to biofilm formation in that they are activated by the overexpression of the biofilm inhibitor Hna [40]. …”

Section: Biological Roles Of Ta Systemsmentioning

confidence: 99%

Structure, Biology, and Therapeutic Application of Toxin–Antitoxin Systems in Pathogenic Bacteria

Lee

2016

Toxins

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Bacterial toxin–antitoxin (TA) systems have received increasing attention for their diverse identities, structures, and functional implications in cell cycle arrest and survival against environmental stresses such as nutrient deficiency, antibiotic treatments, and immune system attacks. In this review, we describe the biological functions and the auto-regulatory mechanisms of six different types of TA systems, among which the type II TA system has been most extensively studied. The functions of type II toxins include mRNA/tRNA cleavage, gyrase/ribosome poison, and protein phosphorylation, which can be neutralized by their cognate antitoxins. We mainly explore the similar but divergent structures of type II TA proteins from 12 important pathogenic bacteria, including various aspects of protein–protein interactions. Accumulating knowledge about the structure–function correlation of TA systems from pathogenic bacteria has facilitated a novel strategy to develop antibiotic drugs that target specific pathogens. These molecules could increase the intrinsic activity of the toxin by artificially interfering with the intermolecular network of the TA systems.

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“…In E. coli , exposure to ciprofloxacin increased TisB toxin levels and, in parallel, increased persister cell numbers [16]. However, the most direct evidence for a role of TA modules in persister cell formation is derived from studies showing that mutations in the HipBA TA system of E. coli can modulate the frequency of persister cell formation [17–19]. Similarly, in M. tuberculosis it has been reported that inactivation of the RelE genes influences the frequency of persister cell formation [20].…”

Section: Introductionmentioning

confidence: 99%

The HicA toxin from Burkholderia pseudomallei has a role in persister cell formation

Butt

Higman

Williams

et al. 2014

Biochemical Journal

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TA (toxin–antitoxin) systems are widely distributed amongst bacteria and are associated with the formation of antibiotic tolerant (persister) cells that may have involvement in chronic and recurrent disease. We show that overexpression of the Burkholderia pseudomallei HicA toxin causes growth arrest and increases the number of persister cells tolerant to ciprofloxacin or ceftazidime. Furthermore, our data show that persistence towards ciprofloxacin or ceftazidime can be differentially modulated depending on the level of induction of HicA expression. Deleting the hicAB locus from B. pseudomallei K96243 significantly reduced persister cell frequencies following exposure to ciprofloxacin, but not ceftazidime. The structure of HicA(H24A) was solved by NMR and forms a dsRBD-like (dsRNA-binding domain-like) fold, composed of a triple-stranded β-sheet, with two helices packed against one face. The surface of the protein is highly positively charged indicative of an RNA-binding protein and His24 and Gly22 were functionality important residues. This is the first study demonstrating a role for the HicAB system in bacterial persistence and the first structure of a HicA protein that has been experimentally characterized.

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Escherichia coli toxin gene hipA affects biofilm formation and DNA release

Cited by 47 publications

References 38 publications

Type II toxin–antitoxin systems are unevenly distributed among Escherichia coli phylogroups

Type II toxin–antitoxin systems are unevenly distributed among Escherichia coli phylogroups

Structure, Biology, and Therapeutic Application of Toxin–Antitoxin Systems in Pathogenic Bacteria

The HicA toxin from Burkholderia pseudomallei has a role in persister cell formation

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