ESCRT Mutant Analysis and Imaging of ESCRT Components in the Model Fungus Ustilago maydis

Haag, Carl; Klein, Thomas; Feldbrügge, Michael

doi:10.1007/978-1-4939-9492-2_19

Cited by 3 publications

(2 citation statements)

References 41 publications

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“…For the phytopathogenic fungus Fusarium graminearum , ESCRT complex are directed toward endocytosis-dependent growth, reproduction, deoxynivalenol production, and full virulence [18]. Moreover, the ESCRT system is involved in hyphal induction and polarity maintenance in Candida albicans, Ustilago maydis and other yeast pathogens [19, 20]. Therefore, ESCRT-regulated MVB biogenesis is required for fungal pathogenicity and morphogenesis.…”

Section: Introductionmentioning

confidence: 99%

ESCRT pathway-dependent MVBs contribute to the morphogenesis of the fungusArthrobotrys oligospora

Tian

Huang

Cui

et al. 2022

Preprint

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Ammonia opens trap formation in the nematode-trapping (NT) fungus Arthrobotrys oligospora, an intriguing morphological switch in NT fungi, where saprophytic mycelia are converted to pathogenic organs. Endocytosis plays a prominent role in nutrient uptake, signaling cascades, and maintenance of cellular homeostasis in higher eukaryotes. Here, we demonstrate that ammonia efficiently promotes endocytosis via the formation of 3D-adhesive mycelial nets in A. oligospora. Trap production is followed by the presence of massive multivesicular bodies (MVBs) and membrane rupture and repair. Additionally, both the ubiquitin-proteasome system and the endosomal sorting complex for transport (ESCRT) pathway are immediately linked to endocytosis regulation and MVB formation in ammonia-induced trap formation. Moreover, disruption of the ESCRT-1 complex subunit proteins AoHse and AoVps27 led to the complete loss of membrane endocytosis and trap formation. Finally, the deletion of the deubiquitinase AoSst2 caused a significant reduction in the number of trap structures produced in response to exposure to ammonia or nematodes. Overall, our results increase our knowledge of the molecular mechanisms underlying the phenotypic changes in the NT fungal group, demonstrating that the endocytosis-ESCRT-MVB pathway participates in the regulation of trapping organs.

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Section: Introductionmentioning

confidence: 99%

ESCRT pathway-dependent MVBs contribute to the morphogenesis of the fungusArthrobotrys oligospora

Tian

Huang

Cui

et al. 2022

Preprint

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“…Genome sequencing (Kämper et al, 2006) revealed that approximately 10% of U. maydis proteins were highly conserved in humans, which had lower similarity or did not even exist in the yeast Saccharomyces cerevisiae (Münsterkötter and Steinberg, 2007). This finding makes U. maydis a unique model organism for many fundamental studies that yeast models do not offer such as endocytosis, long-distance mRNA transport, cell signaling, microtubule organization, and polarized growth (Steinberg and Perez-Martin, 2008;Etxebeste and Espeso, 2016;Haag et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

GC-MS-Based Metabolomics for the Smut Fungus Ustilago maydis: A Comprehensive Method Optimization to Quantify Intracellular Metabolites

Phan

Blank

2020

Front. Mol. Biosci.

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Ustilago maydis, a smut fungus, is an appealing model in fundamental research and an upcoming cell factory for industrial biotechnology. The genome of U. maydis has been sequenced and some synthesis pathways were biochemically described; however, the operation of the cellular metabolic network is not well-characterized. Thus, we conducted a comprehensive study to optimize the sample preparation procedure for metabolomics of U. maydis using GC-MS/MS. Due to the unique characteristics of U. maydis cell culture, two quenching solutions, different washing steps, eight extraction methods, and three derivatization conditions have been examined. The optimal method was then applied for stable isotope-assisted quantification of low molecular weight hydrophilic metabolites while U. maydis utilized different carbon sources including sucrose, glucose, and fructose. This study is the first report on a methodology for absolute quantification of intracellular metabolites in U. maydis central carbon metabolism such as sugars, sugar phosphates, organic acids, amino acids, and nucleotides. For biotechnological use, this method is crucial to exploit the full production potential of this fungus and can also be used to study other fungi of the family Ustilaginaceae.

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Complementing the intrinsic repertoire of Ustilago maydis for degradation of the pectin backbone polygalacturonic acid

Stoffels

Müller

Stachurski

et al. 2020

Journal of Biotechnology

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ESCRT Mutant Analysis and Imaging of ESCRT Components in the Model Fungus Ustilago maydis

Cited by 3 publications

References 41 publications

ESCRT pathway-dependent MVBs contribute to the morphogenesis of the fungusArthrobotrys oligospora

ESCRT pathway-dependent MVBs contribute to the morphogenesis of the fungusArthrobotrys oligospora

GC-MS-Based Metabolomics for the Smut Fungus Ustilago maydis: A Comprehensive Method Optimization to Quantify Intracellular Metabolites

Complementing the intrinsic repertoire of Ustilago maydis for degradation of the pectin backbone polygalacturonic acid

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