EsHO 1 mediated mitigation of NaCl induced oxidative stress and correlation between ROS, antioxidants and HO 1 in seedlings of Eruca sativa: underutilized oil yielding crop of arid region

Abstract

Study have focused on NaCl induced HO 1 production and its co-relation to ROS and antioxidant regulation in Eruca sativa. Seedlings were subjected to NaCl stress ranges from 10 to 150 mM. After 96 h of treatment, plants samples were harvested to evaluate the cellular equilibrium and salt tolerance mechanisms through morphological, stress parameters, non enzymatic and antioxidant enzymes. The HO 1 activity was found to be highest at 75 mM NaCl in leaves and roots which were 2.49 and 2.02 folds respectively. The expression of EsHO 1 was also observed and the higher expression was recorded in roots than leaves. The overall activity of other antioxidants (APX and proline) was also found to be higher at 75 mM concentration. The highest HO 1 activity with other antioxidants indicates the decline in LPX and ROS at 75 mM NaCl. The present study concluded that HO 1 helps in amelioration of NaCl stress by working within a group of antioxidants that create the defense machinery in seedlings of E. sativa by manipulating various physiological processes of plants. These findings for the first time suggest the protective role of HO 1 in scavenging ROS in E. sativa under salinity stress.

Role of Engineered Carbon Nanoparticles (CNPs) in Promoting Growth and Metabolism of Vigna radiata (L.) Wilczek: Insights into the Biochemical and Physiological Responses

“…To overcome the negative impact of CNPs, plants have a well-developed antioxidant system which comprises of several non-enzymatic (proline, carotenoids, thiols) and enzymatic antioxidants (ascorbate peroxidase, catalase, superoxide dismutase, guaiacol peroxidase, glutathione reductase, heme-oxygenase), that can scavenge the surplus ROS. Heme-oxygenase is a novel discovered enzyme in higher plants that works as an antioxidant against different environmental stress, namely salinity, UV-B and heavy metal stress [ 10 , 11 , 12 , 13 ]. The enzyme catalyzes the oxidative degradation of Fe (III) protoporphyrin IX to biliverdin IX (BV), carbon monoxide (CO) and iron in the presence of a reducing equivalent (NADPH) [ 14 , 15 , 16 ].…”

Nitric oxide mitigates salt-induced oxidative stress in Brassica juncea seedlings by regulating ROS metabolism and antioxidant defense system

“…Increasing salinization of soil and water is a global concern which affects the agricultural production worldwide. This problem becomes greater in the region with dry climate, infrequent rainfall and extreme temperature leading to high evapotranspiration rate (Hasanuzzaman et al 2018;Khator et al 2019;Mahawar and Shekhawat 2019). All of these characteristics are common in arid and semiarid regions of India.…”

Exogenous hemin improves Cd2+ tolerance and remediation potential in Vigna radiata by intensifying the HO-1 mediated antioxidant defence system

“…The TI was measured according to the Wilkins 58 method and presented as percent tolerance. The LWC was calculated from seedling biomass by utilizing the equation (fresh weight − dry weight/fresh weight) × 100 59 .…”

“…To check the reproducibility of results, three independent biological replicates were studied following completely randomized design (CRD). The significant variations among treated and untreated seedlings were illustrated at 0.05% significant level by applying Duncan’s Multiple Range test (DMRT) 17 , 59 .…”