Esophageal cancer: Epidemiology, risk factors and screening

Li, Jiang; Xu, Jianguo; Zheng, Yadi; Gao, Ya; He, Siyi; He, Li; Zou, Kaiyong; Li, Ni; Chen, Wanqing; He, Jie

doi:10.21147/j.issn.1000-9604.2021.05.01

Cited by 105 publications

(76 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Esophageal squamous cell carcinoma (ESCC), accounting for about 90% of esophageal cancer (ESCA) cases worldwide, is one of the most common and mortal tumors, especially in East Asia. 1,2 ESCC ranked sixth and fourth, respectively, for morbidity and mortality among all malignant tumors in China. 3 However, among the common tumor types, research on ESCC is comparatively insufficient.…”

Section: Introductionmentioning

confidence: 99%

TLR3 Expression is a Potential Prognosis Biomarker and Shapes the Immune-Active Tumor Microenvironment in Esophageal Squamous Cell Carcinoma

Su¹,

Cai²,

Xiong³

et al. 2022

JIR

View full text Add to dashboard Cite

Background: Toll-like receptor 3 (TLR3) not only plays a crucial role in innate immune and inflammation but also in anti-cancer immunity. Nevertheless, the clinicopathological outcome of TLR3 in ESCC is still ambiguous. Methods: Immunohistochemistry was performed to investigate TLR3 expression and its impact on survival in 137 ESCC patients (including paired esophageal tissues with different stages of early lesions from 37 patients). Furthermore, we downloaded ESCC RNAseq datasets (including phenotype and survival data) from The Cancer Genome Atlas (TCGA). The relationship between TLR3 and prognosis, biological landscape, and immune infiltration was assessed to verify the immunohistochemical results of our tissue samples, explore the possible mechanism of prognostic outcomes, and predict the sensitivity of immunotherapy. Results: TLR3 protein expression displayed an increasing trend in the progression through different grades of cellular atypia, from normal, esophageal simple hyperplasia (ESSH), intraepithelial neoplasia (IEN) to ESCC (P < 0.0083). TLR3 protein had a positive association with inflammation level (Rho = 0.341, P < 0.001). TLR3 mRNA expression was significantly higher in comparison to adjacent normal tissues (P < 0.001). Cox regression analysis indicated high TLR3 protein and mRNA expression conferred good prognosis in our samples and TCGA, especially for advanced ESCC patients (TNM stage III and IV). Overexpression of TLR3 resulted in an immune-active microenvironment via the recruitment of immune-active cells including cytotoxic lymphocytes (CTLs), CD8+ T cells, NK cells, dendritic cells, and M1-type macrophages. TLR3 expression was correlated with the pro-inflammatory cytokines and chemokines relating to anti-tumor immunity. Moreover, GSEA analysis indicated upregulated expression of TLR3 could activate the apoptotic pathway. Conclusion: High TLR3 expression in ESCC patients was associated with a more favorable prognosis, immune-active cell infiltration, and an activated apoptotic pathway. TLR3 has potential applications for immunotherapy and immune response prediction in patients with ESCC.

show abstract

Section: Introductionmentioning

confidence: 99%

TLR3 Expression is a Potential Prognosis Biomarker and Shapes the Immune-Active Tumor Microenvironment in Esophageal Squamous Cell Carcinoma

Su¹,

Cai²,

Xiong³

et al. 2022

JIR

View full text Add to dashboard Cite

show abstract

“… 8 Under this strategy, over 2 million endoscopies have been performed while the detection rate was only 0.9%, less than 2.9% even in regions with extremely high incidence. 6 , 8 , 9 Since screening for ESCC is resource-intensive and has the potential for harm due to the invasive nature of endoscopic examination and biopsy, a precision screening strategy is needed for exclusion of low-risk subgroups from initial screening. 10 The questionnaire-based risk assessment has been accepted as a promising risk enrichment approach to accurately and conveniently identify subjects at high risk for ESCC prior to large-scale endoscopic screening.…”

Section: Introductionmentioning

confidence: 99%

Update and validation of a diagnostic model to identify prevalent malignant lesions in esophagus in general population

et al. 2022

View full text Add to dashboard Cite

“…The incidence and mortality rate of esophageal cancer are the seventh and sixth worldwide, respectively ( 1 ). Esophageal cancer is a highly malignant tumor with a median survival of less than one year and a five-year survival rate of less than 30% for patients with intermediate to advanced disease ( 2 - 4 ).…”

Section: Introductionmentioning

confidence: 99%

Deregulated expression and subcellular localization of CPSF6, a circRNA-binding protein, promote malignant development of esophageal squamous cell carcinoma

Guo¹,

Wang²,

Zhao³

et al. 2022

Chinese Journal of Cancer Research

View full text Add to dashboard Cite

Objective Cleavage and polyadenylation specific factor 6 (CPSF6) has been documented as an oncoprotein in different types of cancer. However, functions of CPSF6 have not been investigated yet in esophageal squamous cell carcinoma (ESCC). Here, we aimed to investigate the potential clinical values and biological functions of CPSF6 in ESCC. Methods For determining the expression level of CPSF6 in ESCC patients, we analyzed published data, performed quantitative real-time polymerase chain reaction (RT-qPCR) and immunohistochemistry assays. Kaplan-Meier curves and log-rank tests were used for survival analyses. GO and KEGG analyses were done for CPSF6-related genes. Cell proliferation, colony formation and xenograft assays were conducted to verify the effects of CPSF6 on ESCC. In addition, cell cycle and apoptosis assays were also performed to manifest the functions of CPSF6 and circCPSF6. RNA pulldown and radioimmunoprecipitation (RIP) assays were used for confirming the interaction between circCPSF6 (hsa_circ_0000417) and CPSF6 protein. The regulatory relationship between CPSF6 protein and circCPSF6 was determined by RT-qPCR. Results We found that CPSF6 was upregulated in ESCC tissues and overexpression of cytoplasmic CPSF6 was associated with poor prognosis. GO and KEGG analyses suggested that CPSF6 could mainly affect cell division in ESCC. Further experiments manifested that CPSF6 promoted cell proliferation and colony formation in vitro . Xenograft assay showed that knockdown of CPSF6 significantly decreased tumor growth rate in vivo . Subsequently, we verified that depletion of CPSF6 led to cell cycle arrest and apoptosis. Finally, we validated that CPSF6, as a circRNA-binding protein, interacted with and regulated its circular isoform circCPSF6 (hsa_circ_0000417), of which depletion also resulted in cell cycle arrest and cell apoptosis in ESCC. Conclusions These findings gave us insight that overexpression of cytoplasmic CPSF6 protein is associated with poor prognosis in ESCC and CPSF6 may function as an oncoprotein, at least in part, through regulating circCPSF6 expression.

show abstract

Esophageal cancer: Epidemiology, risk factors and screening

Cited by 105 publications

References 89 publications

TLR3 Expression is a Potential Prognosis Biomarker and Shapes the Immune-Active Tumor Microenvironment in Esophageal Squamous Cell Carcinoma

TLR3 Expression is a Potential Prognosis Biomarker and Shapes the Immune-Active Tumor Microenvironment in Esophageal Squamous Cell Carcinoma

Update and validation of a diagnostic model to identify prevalent malignant lesions in esophagus in general population

Deregulated expression and subcellular localization of CPSF6, a circRNA-binding protein, promote malignant development of esophageal squamous cell carcinoma

Contact Info

Product

Resources

About