Esophagus tissue engineering: in situ generation of rudimentary tubular vascularized esophageal conduit using the ovine model

Saxena, Amulya K.; Baumgart, Hinrich; Komann, Christian; Ainoedhofer, Herwig; Sołtysiak, Piotr; Kofler, Kristina; Höllwarth, Michael E.

doi:10.1016/j.jpedsurg.2010.02.005

Cited by 43 publications

(30 citation statements)

References 34 publications

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“…For example, Baumert et al [2007] and Saxena et al [2010] used the omentum and the body as an in vivo bioreactor for the tissue engineering of the bladder and cardiac tissue, respectively. Baumert et al [2007] used pig urothelial cells and smooth-muscle cells seeded into sphere-shaped, small-intestinal submucosa matrix grafts transferred into the omentum in the pig after 3 weeks.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Biodegradable Synthetic Vascular Networks and Their Use as a Model of Angiogenesis

Dew¹,

English²,

Ortega

et al. 2016

Cells Tissues Organs

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One of the greatest challenges currently faced in tissue engineering is the incorporation of vascular networks within tissue-engineered constructs. The aim of this study was to develop a technique for producing a perfusable, 3-dimensional, cell-friendly model of vascular structures that could be used to study the factors affecting angiogenesis and vascular biology in engineered systems in more detail. Initially, biodegradable synthetic pseudovascular networks were produced via the combination of robocasting and electrospinning techniques. The internal surfaces of the vascular channels were then recellularized with human dermal microvascular endothelial cells (HDMECs) with and without the presence of human dermal fibroblasts (HDFs) on the outer surface of the scaffold. After 7 days in culture, channels that had been reseeded with HDMECs alone demonstrated irregular cell coverage. However, when using a co-culture of HDMECs inside and HDFs outside the vascular channels, coverage was found to be continuous throughout the internal channel. Using this cell combination, collagen gels loaded with vascular endothelial growth factor were deposited onto the outer surface of the scaffold and cultured for a further 7 days. After this, endothelial cell outgrowth from within the channels into the collagen gel was observed, showing that the engineered vasculature maintains its capacity for angiogenesis. Furthermore, the HDMECs appeared to have formed perfusable tubules within the gel. These results show promising steps towards the development of an in vitro platform for studying angiogenesis and vascular biology in a tissue engineering context.

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Section: Introductionmentioning

confidence: 99%

Fabrication of Biodegradable Synthetic Vascular Networks and Their Use as a Model of Angiogenesis

Dew¹,

English²,

Ortega

et al. 2016

Cells Tissues Organs

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“…2 In some experiments in which a defect was patched, it is unclear if the esophagus was actually engineered or if tissue ingrowth from healthy surrounding tissue bridged the defect. Few techniques have developed any mesenchyme in their attempts and none have identified nerve elements or generated human TEE.…”

Section: Discussionmentioning

confidence: 99%

“…13 Although variable in percentage, the successful tissue engineering of multiple gastrointestinal segments on the same biomaterial highlights its versatility for several progenitor cell populations. This tissue engineering technique is simple and requires only one cell source, EOU, for all of the donor cells (epithelial, fibroblasts, smooth muscle, and nerve cells), unlike other more complex models 2,3,5,6,28,30,36,37 that require cells from numerous sites or even different animals.…”

Section: Ck13mentioning

confidence: 99%

“…1 Later techniques include wrapping absorbable cell-seeded scaffolds around nonabsorbable scaffold tubes and implanting them in vivo; however, this required the eventual removal of the nonabsorbable scaffold tube. 2,3 Previously, the experimental model to address full-thickness esophageal tissue loss has been seeding an inert scaffold with epithelial cells. This approach has the benefit of simplicity and a singular cell source but cannot generate a replacement organ with all cell layers and functions.…”

Section: Introductionmentioning

confidence: 99%

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Murine and Human Tissue-Engineered Esophagus Form from Sufficient Stem/Progenitor Cells and Do Not Require Microdesigned Biomaterials

Spurrier

Speer

Hou

et al. 2015

Tissue Engineering Part A

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Purpose: Tissue-engineered esophagus (TEE) may serve as a therapeutic replacement for absent foregut. Most prior esophagus studies have favored microdesigned biomaterials and yielded epithelial growth alone. None have generated human TEE with mesenchymal components. We hypothesized that sufficient progenitor cells might only require basic support for successful generation of murine and human TEE. Materials and Methods: Esophageal organoid units (EOUs) were isolated from murine or human esophagi and implanted on a polyglycolic acid/poly-l-lactic acid collagen-coated scaffold in adult allogeneic or immunedeficient mice. Alternatively, EOU were cultured for 10 days in vitro prior to implantation. Results: TEE recapitulated all key components of native esophagus with an epithelium and subjacent muscularis. Differentiated suprabasal and proliferative basal layers of esophageal epithelium, muscle, and nerve were identified. Lineage tracing demonstrated that multiple EOU could contribute to the epithelium and mesenchyme of a single TEE. Cultured murine EOU grew as an expanding sphere of proliferative basal cells on a neuromuscular network that demonstrated spontaneous peristalsis in culture. Subsequently, cultured EOU generated TEE. Conclusions: TEE forms after transplantation of mouse and human organ-specific stem/progenitor cells in vivo on a relatively simple biodegradable scaffold. This is a first step toward future human therapies.

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“…Long gap atresia, cancer, Barrett's esophagus, and esophageal strictures and stenosis are pathologic statuses that necessitate esophageal replacement [1]. Many attempts have been made to design and develop artificial esophageal prostheses using appropriate biomaterials.…”

mentioning

confidence: 99%

Tissue engineered esophagus by copper—small intestinal submucosa graft for esophageal repair in a canine model

Tan

Wang

Chen

et al. 2014

Sci. China Life Sci.

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Acellular porcine small intestinal submucosa (SIS) has been used for esophagoplasty with success in a canine model. However, it did not lead to complete epithelialization. For better reconstruction, a cellular component is required. Moreover, promotion of angiogenesis with copper has been widely recognized by basic research as well as clinical studies. In this study, we have evaluated the feasibility and effectiveness of combined Cu and SIS (SIS-Cu patch) for the esophageal repair using a canine model. Eighteen male beagle dogs were subjected to surgical resection to produce cervical esophageal defects (5 cm in length, 180° in range). SIS with Cu (5 or 25 μmol L 1 copper) or without Cu was patched on the esophageal defects. Barium esophagram and histology exam were carried out to evaluate the effectiveness of the therapy. As shown, the SIS-Cu graft promoted re-epithelialization, re-vascularization and muscular regeneration. SIS-Cu patch is more effective than SIS alone for esophageal repair, and the SIS+25 μmol L 1 Cu group demonstrated additional advantages over the SIS+5 μmol L 1

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Esophagus tissue engineering: in situ generation of rudimentary tubular vascularized esophageal conduit using the ovine model

Cited by 43 publications

References 34 publications

Fabrication of Biodegradable Synthetic Vascular Networks and Their Use as a Model of Angiogenesis

Fabrication of Biodegradable Synthetic Vascular Networks and Their Use as a Model of Angiogenesis

Murine and Human Tissue-Engineered Esophagus Form from Sufficient Stem/Progenitor Cells and Do Not Require Microdesigned Biomaterials

Tissue engineered esophagus by copper—small intestinal submucosa graft for esophageal repair in a canine model

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