Essay on the nucleoli survey by the α- and β-satellite DNA probes of the acrocentric chromosomes in mitogen-stimulated human lymphocytes

Demirtaş, Halil; Candemir, Zuhal; Cücer, Nurhan; İmamoğlu, Nalan; Dönmez, Hamiyet; Bökesoy, Işık

doi:10.1016/s0003-3995(00)01014-5

Cited by 9 publications

(3 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The activities of the rRNA genes can be analysed particularly in both interphase and metaphase by using AgNOR or silver staining techniques. Currently, AgNOR/silver staining is the most calculable and reliable method used to determine nucleoli in interphase (Demirtas et al, ; Imamoglu et al, , ; Trerè, ) or active NOR bearing chromosomes at metaphase (Imamoglu et al, ; Sumner, ).…”

Section: Introductionmentioning

confidence: 99%

Nuclear AgNOR protein enhancement in nucleoplasms of peripheral blood lymphocytes of babies/children with down syndrome

Imamoglu

Eröz

Canatan

et al. 2016

Microscopy Res & Technique

Self Cite

View full text Add to dashboard Cite

Down syndrome (DS) is one of the most common chromosomal disorders. The factors contributing to the mental retardation together with other defects in this syndrome have not been fully explained. Individuals with DS have extra rRNA gene family since they carry an extra chromosome 21. The few reports available are on the relationship between the nucleolus organizer regions (NORs) and DS phenotype. The in vivo regulation of NORs expression on the extra chromosome 21 is not completely understood. Previous studies have shown that nucleoli of lymphocytes from infants (mostly neonates) with DS contain more in vivo and in vitro nucleolar AgNOR proteins when compared with healthy infants. The objective of this study is to compare the in vivo nuclear AgNOR protein level in nucleoplasms (also called as karyoplasm) of nonstimulated peripheral blood lymphocytes from babies/children with DS and healthy controls. Peripheral blood samples obtained from 20 babies/children with DS and 20 matched healthy controls were smeared on clean glass slides and then AgNOR staining was performed. The AgNOR protein level in nucleoplasms of lymphocytes from both groups was calculated using a computer program. Nearly 100 interphase nuclei per individual were analysed. Average nuclear AgNOR protein levels in nucleoplasms of lymphocytes from babies/children with DS were found to be significantly higher than those of the controls (P < 0.001). On the basis of our present results, we propose that the increase of nuclear AgNOR protein in in vivo conditions may contribute to the formation of DS phenotypes.

show abstract

Section: Introductionmentioning

confidence: 99%

Nuclear AgNOR protein enhancement in nucleoplasms of peripheral blood lymphocytes of babies/children with down syndrome

Imamoglu

Eröz

Canatan

et al. 2016

Microscopy Res & Technique

Self Cite

View full text Add to dashboard Cite

show abstract

“…AgNOR staining is the most reliable method currently used to visualize/identify nucleoli in interphase nuclei (6,7) or to determine active NOR-bearing chromosomes at metaphase (3). Its evolution as a method of an active ribosomal gene indicator has been explained (8) and reviewed (9).…”

mentioning

confidence: 99%

NOR expression increases on metaphase chromosomes of down syndrome lymphocytes in concordance with mitogen concentration in culture medium

İmamoğlu

Demirtaş

Dönmez-Altuntaş

et al. 2005

Cytometry Part B Clinical

Self Cite

View full text Add to dashboard Cite

“…İnterfazdaki AgNOR boyama, NOR+ durumdaki akrosentriklerin çekirdek içindeki toptan konumlarını da gösterir ve bunlar, ince yapısı önemli ölçüde bilinen çekirdekçikle birlikte bulunurlar. Çekirdekçik yapısı da sabit olmayıp, hücrenin interfazdaki evrelerine göre sürekli bir değişim içindedir (8). Bu da aktif NOR taşıyan akrosentriklerin, çekirdek ve çekirdekçik içindeki konumlarının interfaz evresine göre değişebileceklerini işaret eder.…”

Section: Introductionunclassified

Comparative Studies of the AgNORS Motifs in Phytohemagglutinin-Stimulated Human T-Lymphocytes with T-Lymphocyte Subgroups

Bıtgen¹,

Dönmez-Altuntaş²,

Hamurcu³

et al. 2012

Erciyes Med J

Self Cite

View full text Add to dashboard Cite

Objective: Using of argyrophilic nucleolar organiser region (AgNOR) patterns, instead of the fluorescent T-lymphocyte markers, in specification of subgroups of T-lymphocyte was researched in the study. For this, it was aimed to indicate that AgNOR pattern of each T-lymphocyte subgroup remains stable in a stage of cell cycle stimulated by phytohaemagglutinin (PHA) and that these patterns are different from each other. Material and Methods: Peripheral blood samples of four healthy volunteers were cultured according to whole blood and/or isolated blood culture methods. The slides were stained by using AgNOR and immunohistochemical staining methods. Results: As we expected, the CD8 staining is not observed in a single style of the nucleolar organiser region (NOR) motif, but rather occurred in the forms of different kinds of nucleolus. It was found that the different types of NOR motifs formed with AgNOR staining are not related to the T-lymphocyte subgroup. Conclusion: It was concluded that the NOR motifs are not specific to subgroups of T-lymphocytes. Further investigations are needed to support our results.

show abstract

Essay on the nucleoli survey by the α- and β-satellite DNA probes of the acrocentric chromosomes in mitogen-stimulated human lymphocytes

Cited by 9 publications

References 52 publications

Nuclear AgNOR protein enhancement in nucleoplasms of peripheral blood lymphocytes of babies/children with down syndrome

Nuclear AgNOR protein enhancement in nucleoplasms of peripheral blood lymphocytes of babies/children with down syndrome

NOR expression increases on metaphase chromosomes of down syndrome lymphocytes in concordance with mitogen concentration in culture medium

Comparative Studies of the AgNORS Motifs in Phytohemagglutinin-Stimulated Human T-Lymphocytes with T-Lymphocyte Subgroups

Contact Info

Product

Resources

About