Essential dynamic interdependence of FtsZ and SepF for Z-ring and septum formation in Corynebacterium glutamicum

Sogues, Adrià; Martínez, Mariano; Gaday, Quentin; Assaya, Mathilde Ben; Graña, Martín; Voegele, Alexis; VanNieuwenhze, Michael S.; England, Patrick; Haouz, Ahmed; Chenal, Alexandre; Trépout, Sylvain; Durán, Rosario; Wehenkel, Annemarie; Alzari, P.M.

doi:10.1038/s41467-020-15490-8

Cited by 34 publications

(78 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For ectopic expression in C. glutamicum , the pbp2m gene was amplified from C. diphtheriae strain FRC0402 and put under the control of the inducible P gntK promoter on the shuttle vector pTGR5 [ 58 ] (Additional file 4 : Fig. S1) .…”

Section: Methodsmentioning

confidence: 99%

Population genomics and antimicrobial resistance in Corynebacterium diphtheriae

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background Corynebacterium diphtheriae, the agent of diphtheria, is a genetically diverse bacterial species. Although antimicrobial resistance has emerged against several drugs including first-line penicillin, the genomic determinants and population dynamics of resistance are largely unknown for this neglected human pathogen. Methods Here, we analyzed the associations of antimicrobial susceptibility phenotypes, diphtheria toxin production, and genomic features in C. diphtheriae. We used 247 strains collected over several decades in multiple world regions, including the 163 clinical isolates collected prospectively from 2008 to 2017 in France mainland and overseas territories. Results Phylogenetic analysis revealed multiple deep-branching sublineages, grouped into a Mitis lineage strongly associated with diphtheria toxin production and a largely toxin gene-negative Gravis lineage with few toxin-producing isolates including the 1990s ex-Soviet Union outbreak strain. The distribution of susceptibility phenotypes allowed proposing ecological cutoffs for most of the 19 agents tested, thereby defining acquired antimicrobial resistance. Penicillin resistance was found in 17.2% of prospective isolates. Seventeen (10.4%) prospective isolates were multidrug-resistant (≥ 3 antimicrobial categories), including four isolates resistant to penicillin and macrolides. Homologous recombination was frequent (r/m = 5), and horizontal gene transfer contributed to the emergence of antimicrobial resistance in multiple sublineages. Genome-wide association mapping uncovered genetic factors of resistance, including an accessory penicillin-binding protein (PBP2m) located in diverse genomic contexts. Gene pbp2m is widespread in other Corynebacterium species, and its expression in C. glutamicum demonstrated its effect against several beta-lactams. A novel 73-kb C. diphtheriae multiresistance plasmid was discovered. Conclusions This work uncovers the dynamics of antimicrobial resistance in C. diphtheriae in the context of phylogenetic structure, biovar, and diphtheria toxin production and provides a blueprint to analyze re-emerging diphtheria.

show abstract

Section: Methodsmentioning

confidence: 99%

Population genomics and antimicrobial resistance in Corynebacterium diphtheriae

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…In B. subtilis , SepF is non-essential and has an overlapping role with FtsA, as overexpression of SepF can rescue an FtsA depletion strain 16 , 17 . In contrast, in other bacterial lineages where FtsA is absent, such as most Actinobacteria and Cyanobacteria , SepF is an essential component of the early divisome, orchestrating Z-ring assembly and participating in membrane remodeling 18 – 21 . The first crystal structure of a bacterial SepF-FtsZ complex was only recently obtained from the Actinobacterium Corynebacterium glutamicum 21 .…”

Section: Introductionmentioning

confidence: 98%

“…In contrast, in other bacterial lineages where FtsA is absent, such as most Actinobacteria and Cyanobacteria , SepF is an essential component of the early divisome, orchestrating Z-ring assembly and participating in membrane remodeling 18 – 21 . The first crystal structure of a bacterial SepF-FtsZ complex was only recently obtained from the Actinobacterium Corynebacterium glutamicum 21 . It showed that SepF forms a functional dimer that is required for FtsZ binding 21 .…”

Section: Introductionmentioning

confidence: 98%

“…The first crystal structure of a bacterial SepF-FtsZ complex was only recently obtained from the Actinobacterium Corynebacterium glutamicum 21 . It showed that SepF forms a functional dimer that is required for FtsZ binding 21 . C. glutamicum SepF binds to the FtsZ CTD through a conserved pocket and interacts with residues at the α-helical interface of the functional dimer 21 .…”

Section: Introductionmentioning

confidence: 99%

“…It showed that SepF forms a functional dimer that is required for FtsZ binding 21 . C. glutamicum SepF binds to the FtsZ CTD through a conserved pocket and interacts with residues at the α-helical interface of the functional dimer 21 .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

SepF is the FtsZ anchor in archaea, with features of an ancestral cell division system

et al. 2021

Self Cite

View full text Add to dashboard Cite

Most archaea divide by binary fission using an FtsZ-based system similar to that of bacteria, but they lack many of the divisome components described in model bacterial organisms. Notably, among the multiple factors that tether FtsZ to the membrane during bacterial cell constriction, archaea only possess SepF-like homologs. Here, we combine structural, cellular, and evolutionary analyses to demonstrate that SepF is the FtsZ anchor in the human-associated archaeon Methanobrevibacter smithii. 3D super-resolution microscopy and quantitative analysis of immunolabeled cells show that SepF transiently co-localizes with FtsZ at the septum and possibly primes the future division plane. M. smithii SepF binds to membranes and to FtsZ, inducing filament bundling. High-resolution crystal structures of archaeal SepF alone and in complex with the FtsZ C-terminal domain (FtsZCTD) reveal that SepF forms a dimer with a homodimerization interface driving a binding mode that is different from that previously reported in bacteria. Phylogenetic analyses of SepF and FtsZ from bacteria and archaea indicate that the two proteins may date back to the Last Universal Common Ancestor (LUCA), and we speculate that the archaeal mode of SepF/FtsZ interaction might reflect an ancestral feature. Our results provide insights into the mechanisms of archaeal cell division and pave the way for a better understanding of the processes underlying the divide between the two prokaryotic domains.

show abstract

Creating Polyploid Escherichia Coli and Its Application in Efficient L‐Threonine Production

Wang,

Chen,

Jin

et al. 2023

Advanced Science

View full text Add to dashboard Cite

Prokaryotic genomes are generally organized in haploid. In synthetic biological research, efficient chassis cells must be constructed to produce bio‐based products. Here, the essential division of the ftsZ gene to create functional polyploid E. coli is regulated. The artificial polyploid E. coli containing 2–4 chromosomes is confirmed through PCR amplification, terminator localization, and flow cytometry. The polyploid E. coli exhibits a larger cell size, and its low pH tolerance and acetate resistance are stronger than those of haploid E. coli. Transcriptome analysis shows that the genes of the cell's main functional pathways are significantly upregulated in the polyploid E. coli. These advantages of the polyploid E. coli results in the highest reported L‐threonine yield (160.3 g L−1) in fed‐batch fermentation to date. In summary, an easy and convenient method for constructing polyploid E. coli and demonstrated its application in L‐threonine production is developed. This work provides a new approach for creating an excellent host strain for biochemical production and studying the evolution of prokaryotes and their chromosome functions.

show abstract

Essential dynamic interdependence of FtsZ and SepF for Z-ring and septum formation in Corynebacterium glutamicum

Cited by 34 publications

References 82 publications

Population genomics and antimicrobial resistance in Corynebacterium diphtheriae

Population genomics and antimicrobial resistance in Corynebacterium diphtheriae

SepF is the FtsZ anchor in archaea, with features of an ancestral cell division system

Creating Polyploid Escherichia Coli and Its Application in Efficient L‐Threonine Production

Contact Info

Product

Resources

About