Essential Oil from the Leaves of Annona vepretorum: Chemical Composition and Bioactivity

Abstract

The essential oil from the leaves of Annona vepretorun was obtained by hydrodistillation using a Clevenger-type apparatus and analyzed by GC-MS and GC-FID. Eighteen compounds representing 98.1% of the crude essential oil were identified. The major compounds identified were bicyclogermacrene (43.7%), spathulenol (11.4%), -felandrene (10.0%), -pinene (7.1%), (E)-β-ocimene (6.8%), germacrene D (5.8%), and p-cymene (4.2%). The trypanocidal activity against Trypanosoma cruzi epimastigote forms, as well as, the antimicrobial and antioxidant proprieties was investigated. The essential oil showed a potent trypanocidal activity with IC 50 value of 31.9±1.3µg.mL-1. For antimicrobial activity, the best result was observed against Candida tropicalis with a MIC value of 100µg.mL-1. For antioxidant capacity the essential oil showed weak activity.

Chemical composition of essential oils fromAnnona vepretorumMart. andAnnona squamosaL. (Annonaceae) leaves and their antimalarial and trypanocidal activities

“…Although the essential oils were less effective than benznidazole or mefloquine in the biological assays, it is important to say that these reference drugs are highly toxic to hosts and showing a variable efficacy in several strains that have some degree of resistance to these drugs (18,19). Furthermore, results presented in this study are considered very promising when compared with other results of Annonaceae essential oils with trypanocidal and antimalarial activity (6,16,20). Journal of Essential Oil Research 5 After confirming that AVOE and ASOE were able to kill parasites, our next step was to understand how they affect parasite cells through assays with trypomastigotes forms of T. cruzi, once the trypanocidal activity of species of Annonaceae is better documented than the antimalarial activity.…”

“…The major compounds identified in ASOE essential oil were (E)-caryophyllene (27.4%), germacrene D (17.1%), bicyclogermacrene (10.8%), (Z)-caryophyllene (7.3%), β-elemene (6.2%), α-humulene (5.7%), epi-α-cadinol (4.3%), γ-cadinene (4.2%) and δ-elemene (4.1%) ( Table 1). Recently, Costa et al (6) described the chemical composition of the essential oil from the leaves of AVOE, identifying eighteen compounds less than this study. These variations, or detector sensitivity or sample dilution in the composition of the major constituents, as well as the contents of all components, can be related to soil and climate conditions, water stress, collection place, nutrition and other abiotic factors (16).…”

“…Previous chemical and pharmacological investigations of some species from this genus have indicated the presence of important bioactive compounds (alkaloids, acetogenins and terpenes) that exhibit various pharmacological activities, including cytotoxicity against tumor cell lines, antimicrobial and particularly trypanocidal properties (5)(6)(7).…”

Antitumour Activity of the Microencapsulation of Annona vepretorum Essential Oil

“…Four aporphine alkaloids, liriodenine, oxonantenine, lanuginosine and lysicamine, as well as 1,3,6,6‐tetramethyl‐5,6,7,8‐tetrahydro‐isoquinolin‐8‐one and vomifoliol, were also found in A. vepretorum leaves . Additionally, the leaf essential oil shows trypanocidal, antimalarial, antifungal and antioxidant properties, and it primarily includes the presence of sesquiterpene and monoterpene compounds . The chemical composition and antitumour potential of essential oil of A. vepretorum leaf alone (free EO) and complexed with β‐cyclodextrin (EO complexed) were investigated in this study.…”

Orofacial antinociceptive effect of the ethanolic extract of Annona vepretorum Mart. (Annonaceae)

“…Annona vepretorum is an endemic species of Brazil (Caatinga biome), popularly known as "araticum" and "pinha da Caatinga", and is widely used in human nutrition. The fruits are usually consumed as such or in juices [9]. The roots are popularly used against bee stings and are employed as a natural anti-inflammatory [10].…”