Essential oil nanoemulsions: antibacterial activity in contaminated fruit juices

Abstract

Summary Many human acid tolerant bacterial and fungal pathogens can be transmitted through the consumption of the contaminated fruit juices. We aim to formulate essential oil nanoemulsions (basil, black seed, turmeric, clove & cinnamon), determine their ability to clear contamination by food borne bacterial pathogens from fruit juices. The antibacterial activity of the optimised formulations was tested in the fruit juices against bacterial pathogens causing gastrointestinal tract infections. The minimum bactericidal concentration (MBC) of clove emulsions ranged from 15.6 to 25 μL mL−1. Cinnamon oil emulsion had an MBC ranging between 15 and 31 μL mL−1. At MBC, cinnamon oil emulsions caused a 6log10 decrease in viable counts by 8 h and maintained the sterility of fruit juices for 7 days at ambient temperature. Thus, clove and cinnamon microemulsions can be used as juice additives to control food borne bacterial pathogens and maintain the bacterial sterility of fruit juices.

Anticancer effects of enrichment in the bioactive components of the functional beverage of Turkish gastronomy by supplementation with purple basil (Ocimum basilicumL.) and the ultrasound treatment

“…Ocimium Basilicum L. known as purple basil (PB) is a member of the Lamiaceae family that has long been used for both seasoning to enhance the flavors of foods and remedies of variety of diseases as complementary and alternative medicine. Previous studies suggested that PB could have potential clinical applications for treating diseases related to cancer, allergy, inflammation, and infections against bacterial, fungal, and viral due to its naturally occurring food compositions rich in polyphenol, aromatic compounds, and essential oils (Franklyne et al., 2019; Torpol et al., 2019; Złotek et al., 2016).…”

Synergistic properties of Eucalyptus caesia and Dracocephalum multicaule Montbr & Auch essential oils: Antimicrobial activity against food borne pathogens and antioxidant activity in pear slices

“…For P. aeruginosa , D. multicaule EO alone showed the least inhibitory effect and no significant difference was observed between the other treatments at 25°C ( p > .05). Franklyne, Iyer, Ebenazer, Mukherjee, and Chandrasekaran (2019) examined the antibacterial effect of turmeric, basil, clove, black seed, and cinnamon essential oils and their nanoemulsions in fruit juices. They reported that among the essential oils, clove, and cinnamon showed the highest antibacterial activity against the pathogenic bacteria and cinnamon oil emulsions maintained the sterility of fruit juices for 7 days at ambient temperature.…”

Nanotechnology Based Approaches in Phage Therapy: Overcoming the Pharmacological Barriers

“…Nanoemulsions thus offer a new method of encapsulating sensitive molecules such as proteins, enzymes, phages in a nanoporous matrix. The confinement of bio-molecules within nanoemulsions causes a change in the surrounding a w (water activity) which imparts higher stability during storage period ( Franklyne et al, 2019 ). Such multiple emulsion formulations can act as a suitable housing system shielding the phage from inactivation from immune system, outer proteolytic enzymes while maintaining their structural and functional activity ( Balcão et al, 2014 ; Esteban et al, 2014 ; Sawant et al, 2017 ).…”