Essential oil of Chrysanthemum indicum L.: potential biocontrol agent against plant pathogen Phytophthora nicotianae

Han, Xiaobin; Zhao, Jian; Cao, Jianmin; Zhang, Chengsheng

doi:10.1007/s11356-019-04152-y

Cited by 37 publications

(42 citation statements)

References 36 publications

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“…The tested tobacco variety was Xiaohuangjin 1025, which is susceptible to tobacco black shank disease. P. nicotianae was cultured in oat medium (OA) according to a method described previously by Han et al [20].…”

Section: Methodsmentioning

confidence: 99%

“…Headspace solid phase microextraction (HS-SPME) was chosen as the method of volatile compounds sampling [20]. The so-called sandwich type (DVB/CAR/PDMS, Supelco, Bellefonte, PA, USA) fiber was used for the absorption of essential oil volatile compounds.…”

Section: Methodsmentioning

confidence: 99%

“…To evaluate DADS-induced cell membrane damage, a propidium iodide (PI) fluorescence assay was conducted [20,26]. Phytophthora nicotianae mycelia were harvested as follows.…”

Section: Methodsmentioning

confidence: 99%

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The Antifungal Effect of Garlic Essential Oil on Phytophthora nicotianae and the Inhibitory Component Involved

et al. 2019

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This study explored the chemical compositions of garlic essential oil, the inhibitory activity of garlic essential oil and diallyl disulfide (DADS) against Phytophthora nicotianae, and the effects on mycelial plasma membrane permeability and P. nicotianae inhibition. In total, 29 compounds were detected in garlic essential oil, of which 26 were detected by gas chromatography‒mass spectrometry (GC-MS) and 21 by headspace solid-phase microextraction (HS-SPME) GC-MS. DADS (60.12% and 19.09%) and trisulfide di-2-propenyl (14.18% and 17.98%) were the major components identified by HS-SPME GC-MS and GC-MS analysis, respectively. Half-inhibitory concentration (Ec50, antagonism) and minimum inhibitory concentration (MIC, fumigation) of DADS against P. nicotianae were 150.83 μL/L and 20 μL/L, respectively, while Ec50 of garlic essential oil was 1108.25 μL/L. Mycelial membrane permeability gradually increased in a concentration-dependent manner, and cell death increased at 450 μL/L DADS. Furthermore, DADS treatment significantly reduced the incidence of tobacco black shank and the number of P. nicotianae pathogens in rhizosphere soil. DADS also promoted root development of tobacco seedlings at low concentrations, which was inhibited at high concentrations. Therefore, DADS may play an important role in the antifungal effect against P. nicotianae by destroying mycelial cell membrane integrity, causing an increase in cell membrane permeability, and leading to cell death.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The Antifungal Effect of Garlic Essential Oil on Phytophthora nicotianae and the Inhibitory Component Involved

et al. 2019

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“…Through zoospores and oospores (Tan et al, 2018), pathogens infect the internal tissues of plants from roots, stems and leaves, thereby disrupting plant metabolism and leading to wilting, chlorisis and even death of the host plant (Gallup et al, 2018). Systemic diseases causing vascular bundle lesions in plants usually require the use of systemic fungicides for control (Han et al, 2019). Therefore, the selection of appropriate fungicides is of great significance for the control of diseases caused by P. nicotianae.…”

Section: Introductionmentioning

confidence: 99%

Effects of manganese and zinc on the growth process of Phytophthora nicotianae and the possible inhibitory mechanisms

Luo

Yao

Tan

et al. 2020

PeerJ

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Background Phytophthora nicotianae is a fungal soil-borne pathogen that damages various plant species. Mancozeb and Zineb, fungicides containing manganese (Mn) and zinc (Zn) as the main components, are widely used to control the diseases caused by Phytophthora. However, the inhibition mechanism is still unclear. The purpose of this study was to examine the effects of Mn and Zn on P. nicotianae and to determine possible inhibitory mechanisms of Mn and Zn on sporangiogenesis of P. nicotianae. Methods The mycelial growth, sporangium generation, zoosporogenesis and zoospore germination of P. nicotianae were observed under Mn and Zn treatments. The gene (csn4 and csn7) expression levels of P. nicotianae in different growth stages were examined. Csn4 and csn7 gene expression, superoxide dismutase (SOD) activity, catalase (CAT) activity and malondialdehyde (MDA) content were tested at the stage of sporangiogenesis under different Mn and Zn concentrations. Results Mycelial growth of P. nicotianae was significantly inhibited by Mn from ≥1 mg/L concentration and by Zn from ≥10 mg/L. The sporangia production, sporangia release, and zoospore germination of P. nicotianae were significantly reduced by Mn at all concentrations, while treatment with Zn from ≥0.5 mg/L concentration significantly inhibited the same processes. At the same concentration, the inhibition rate of Mn on the growth process of P. nicotianae was higher than that of Zn. The csn4 and csn7 gene transcription of P. nicotianae were significantly reduced by all treatments with Mn and Zn at the stage of sporangiogenesis. With the increase of Mn concentration, the activities of SOD and CAT increased to maxima and then decreased, and the content of MDA gradually increased during sporangiogenesis of P. nicotianae. The sporangia production of P. nicotianae was significantly positively correlated with the expression levels of the genes csn4 and csn7. Conclusion The inhibitory effect of Mn on the growth process of P. nicotianae was stronger than that of Zn, especially on sporangiogenesis and zoosporogenesis. A possible mechanism of the inhibitory effect on sporangiogenesis of P. nicotianae was that Mn and Zn acted by inhibiting the expression levels of the genes csn4 and csn7 and by affecting antioxidant enzyme activity (further resulting in lipid peroxidation) in the sporangium of P. nicotianae.

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“…Therefore, replacing synthetic chemical fungicides by botanical alternatives could be a suitable choice to avoid toxicological and environmental-related issues. For instance, the botanical secondary metabolites, such as alkaloids [25], flavonoids [26] and volatile organic compounds [27], may act as antibacterial active substances to inhibit plant diseases. Therefore, botanical fungicides have been considered as environmentally friendly alternatives to synthetic chemical fungicides.…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition of a Supercritical Fluid (Sfe-CO2) Extract from Baeckea frutescens L. Leaves and Its Bioactivity Against Two Pathogenic Fungi Isolated from the Tea Plant (Camellia sinensis (L.) O. Kuntze)

Jiang

Zhang

Qin

et al. 2020

Plants

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Colletotrichum gloeosporioides and Pseudopestalotiopsis camelliae-sinensis are the two most important tea plant (Camellia sinensis L.) pathogenic fungi. Interest in natural plant extracts as alternatives to synthetic chemical fungicides to control plant pathogens is growing. In this study, the volatile fraction of Baeckea frutescens L. was extracted by supercritical fluid extraction (SFE-CO2), and its chemical composition was analyzed, and investigated for its antifungal activity against C. gloeosporioides and P. camelliae. The major constituents of the volatile fraction were β-caryophyllene (28.05%), α-caryophyllene (24.02%), δ-cadinene (6.29%) and eucalyptol (5.46%) in B. frutescens SFE-CO2 extracts. The terpineol, linalool, terpinen-4-ol and eucalyptol showed strong contact antifungal activity against P. camelliae and C. gloeosporioides with median inhibitory concentration (MIC50) in the range of 0.69 μL/mL to 2.79 μL/mL and 0.62 μL/mL to 2.18 μL/mL, respectively. Additionally, the volatile fraction had high fumigation antifungal activity against P. camelliae and C. gloeosporioides with an inhibition rate between 20.87% and 92.91%. Terpineol presented the highest antifungal activity in the contact and fumigation toxicity assays. Terpineol, linalool, terpinen-4-ol and eucalyptol were associated with the most active chemical compounds in the volatile fraction against the fungi. The results suggest that B. frutescens SFE-CO2 extracts are potential ingredients to develop a natural fungicide for control of tea plant pathogens.

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Essential oil of Chrysanthemum indicum L.: potential biocontrol agent against plant pathogen Phytophthora nicotianae

Cited by 37 publications

References 36 publications

The Antifungal Effect of Garlic Essential Oil on Phytophthora nicotianae and the Inhibitory Component Involved

The Antifungal Effect of Garlic Essential Oil on Phytophthora nicotianae and the Inhibitory Component Involved

Effects of manganese and zinc on the growth process of Phytophthora nicotianae and the possible inhibitory mechanisms

Chemical Composition of a Supercritical Fluid (Sfe-CO2) Extract from Baeckea frutescens L. Leaves and Its Bioactivity Against Two Pathogenic Fungi Isolated from the Tea Plant (Camellia sinensis (L.) O. Kuntze)

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