Essential oil of Mentha pulegium induces anthelmintic effects and reduces parasite-associated oxidative stress in rodent model

Sebai, Essia; Abidi, Anouar; Serairi, Raja; Marzouki, Moomen; Saratsi, Katerina; Darghouth, Mohamed Aziz; Sotiraki, Smaragda; Akkari, Hafidh

doi:10.1016/j.exppara.2021.108105

Cited by 17 publications

(6 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results are further supported by scanning electron microscopy of larval and adult worm stages treated with the CS-PLGA particles, showing that adsorption of particles to the cuticle was associated with considerable structural damage, particularly with the nanoscale particles due to their ability to penetrate and permeate the cuticle. A similar observation was reported in a previous study [ 57 ], attributing this effect to the affinity of the hydrophobic EO components to the cell membrane, which alters the electrostatic balance and increases the cuticle’s permeability and damage of cuticular protein.…”

Section: Discussionsupporting

confidence: 88%

“…The inhibition of parasite motility is considered the gold standard for evaluating the anthelmintic activity of components of EOs either in non-encapsulated (free) [ 57 ] or encapsulated form [ 58 ]. In the present study, we found that free, combined EO components (linalool, geraniol, and eugenol) have a strong effect on larval motility, reaching 96.1% inhibition, compared with EO components encapsulated in uncoated and CS-PLGA particles (75% and 76.9%, respectively), which may be related to the slow release of EO components from the PLGA particles.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Microfluidic-Based Formulation of Essential Oils-Loaded Chitosan Coated PLGA Particles Enhances Their Bioavailability and Nematocidal Activity

et al. 2022

View full text Add to dashboard Cite

In this study, poly (lactic-co-glycolic) acid (PLGA) particles were synthesized and coated with chitosan. Three essential oil (EO) components (eugenol, linalool, and geraniol) were entrapped inside these PLGA particles by using the continuous flow-focusing microfluidic method and a partially water-miscible solvent mixture (dichloromethane: acetone mixture (1:10)). Encapsulation of EO components in PLGA particles was confirmed by Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, with encapsulation efficiencies 95.14%, 79.68%, and 71.34% and loading capacities 8.88%, 8.38%, and 5.65% in particles entrapped with eugenol, linalool, and geraniol, respectively. The EO components’ dissociation from the loaded particles exhibited an initial burst release in the first 8 h followed by a sustained release phase at significantly slower rates from the coated particles, extending beyond 5 days. The EO components encapsulated in chitosan coated particles up to 5 μg/mL were not cytotoxic to bovine gut cell line (FFKD-1-R) and had no adverse effect on cell growth and membrane integrity compared with free EO components or uncoated particles. Chitosan coated PLGA particles loaded with combined EO components (10 µg/mL) significantly inhibited the motility of the larval stage of Haemonchus contortus and Trichostrongylus axei by 76.9%, and completely inhibited the motility of adult worms (p < 0.05). This nematocidal effect was accompanied by considerable cuticular damage in the treated worms, reflecting a synergistic effect of the combined EO components and an additive effect of chitosan. These results show that encapsulation of EO components, with a potent anthelmintic activity, in chitosan coated PLGA particles improve the bioavailability and efficacy of EO components against ovine gastrointestinal nematodes.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Microfluidic-Based Formulation of Essential Oils-Loaded Chitosan Coated PLGA Particles Enhances Their Bioavailability and Nematocidal Activity

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Similar components were also found in the leaves and flowers of small wild plants growing in Saudi Arabia named Lantana camara , Anvillea garcinii , and Strychnos nux-vomica [ 8 , 9 , 10 ]. In vitro studies showed that mint extracts from the Tunisian genus exhibited important antioxidant, antiviral, antiallergenic, and antimicrobial activities [ 11 , 12 , 13 ]. In addition, previous studies have demonstrated the potent antitumor activities of the Mentha species [ 14 , 15 ] as an effective chemopreventive agent [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Tunisian Native Mentha pulegium L. Extracts: Phytochemical Composition and Biological Activities

et al. 2022

View full text Add to dashboard Cite

Mint species (Lamiaceae family) have been used as traditional remedies for the treatment of several diseases. In this work, we aimed to characterize the biological activities of the total phenolic and flavonoid contents of Mentha pulegium L. extracts collected from two different regions of Tunisia. The highest amounts of total phenols (74.45 ± 0.01 mg GAE/g DW), flavonoids (28.87 ± 0.02 mg RE/g DW), and condensed tannins (4.35 ± 0.02 mg CE/g DW) were found in the Bizerte locality. Methanolic leaf extracts were subjected to HPLC-UV analysis in order to identify and quantify the phenolic composition. This technique allowed us to identify seven phenolic compounds: two phenolic acids and five flavonoid compounds, such as eriocitrin, hesperidin, narirutin, luteolin, and isorhoifolin, which were found in both extracts with significant differences between samples collected from the different regions (p < 0.05). Furthermore, our results showed that the methanolic extract from leaves collected from Bizerte had the highest antioxidant activities (DPPH IC50 value of 16.31 μg/mL and 570.08 μmol Fe2+/g, respectively). Both extracts showed high radical-scavenging activity as well as significant antimicrobial activity against eight tested bacteria. The highest antimicrobial activities were observed against Gram-positive bacteria with inhibition zone diameters and MIC values ranging between 19 and 32 mm and 40 and 160 µg/mL, respectively. Interestingly, at 10 μg/mL, the extract had a significant effect on cell proliferation of U87 human glioblastoma cells. These findings open perspectives for the use of Mentha pulegium L. extract in green pharmacy, alternative/complementary medicine, and natural preventive therapies for the development of effective antioxidant, antibacterial, and/or antitumoral drugs.

show abstract

“…Similarly, the anesthetic potential of M. piperita was demonstrated by Brandão et al [9]. Interestingly, several phytochemical studies showed that the members of the genus Mentha contain important classes of volatile and phenolic components [10][11][12][13][14][15][16][17]. In particular, the essential oils were reported to be cytotoxic and antibacterial agents, whereas polar extracts showed antioxidant properties [7,[18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 95%

Novel Perceptions on Chemical Profile and Biopharmaceutical Properties of Mentha spicata Extracts: Adding Missing Pieces to the Scientific Puzzle

Zengin

Ceylan

et al. 2022

Plants

View full text Add to dashboard Cite

Mentha spicata is one of the most popular species in the genus, and it is of great interest as a gastrointestinal and sedative agent in the folk medicine system. In this study, different M. spicata extracts, obtained by the use of four solvents (hexane, chloroform, acetone and acetone/water) were chemically characterized using HPLC-ESI-MS n, which allowed for identification of 27 phenolic compounds. The extracts’ antioxidant and enzyme inhibitory properties were investigated. In addition, neuroprotective effects were evaluated in hypothalamic HypoE22 cells, and the ability of the extracts to prevent the hydrogen peroxide-induced degradation of dopamine and serotonin was observed. The best antioxidant effect was achieved for all the extraction methods using acetone/water as a solvent. These extracts were the richest in acacetin, eriodictyol, hesperidin, sagerinic acid, naringenin, luteolin, chlorogenic acid, chrysoeriol and apigenin. The intrinsic antioxidant and enzyme inhibition properties of the acetone/water extract could also explain, albeit partially, its efficacy in preventing prostaglandin E2 overproduction and dopamine depletion (82.9% turnover reduction) in HypoE22 cells exposed to hydrogen peroxide. Thus, our observations can provide a scientific confirmation of the neuromodulatory and neuroprotective effects of M. spicata.

show abstract

Essential oil of Mentha pulegium induces anthelmintic effects and reduces parasite-associated oxidative stress in rodent model

Cited by 17 publications

References 65 publications

Microfluidic-Based Formulation of Essential Oils-Loaded Chitosan Coated PLGA Particles Enhances Their Bioavailability and Nematocidal Activity

Microfluidic-Based Formulation of Essential Oils-Loaded Chitosan Coated PLGA Particles Enhances Their Bioavailability and Nematocidal Activity

Tunisian Native Mentha pulegium L. Extracts: Phytochemical Composition and Biological Activities

Novel Perceptions on Chemical Profile and Biopharmaceutical Properties of Mentha spicata Extracts: Adding Missing Pieces to the Scientific Puzzle

Contact Info

Product

Resources

About