Essential Oils ofProtiumof the Adolpho Ducke Forest Reserve:Protium crassipetalum, P. heptaphyllumsubs.ulei, P. pilosissimumandP. polybotryum

Carvalho, Loretta Ennes de; Magalhães, Lyege Amazonas Maciel; Lima, Maria da Paz; Marques, Márcia Ortiz Mayo; Facanali, Roselaine

doi:10.1080/0972060x.2013.831557

Cited by 6 publications

(3 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Relative concentration of spathulenol, by comparison with the concentration found in P. ovatum leaves (17.6 %), was lower in essential oils from P. hebetatum leaves and branches (Pinto et al 2010), P. pilosum leaves and P. strumosum resins (Zoghbi et al 2005). In the analysis of the chemical composition of the essential oil from P. heptaphyllum leaves, sesquiterpene spathulenol was identified as one of the major constituents (Carvalho et al 2013). Results of the evaluation of the leishmanicidal activity of the essential oil from P. ovatum leaves against the promastigote forms of L. amazonensis are shown in Table II. IC 50 value = 2.28 μg.mL -1 is considered to be very promising by comparison with that of Amphotericin B (IC 50 = 0.60 μg.mL -1 ) ( Table II).…”

Section: Resultsmentioning

confidence: 99%

In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae)

Estevam

Deus

Silva

et al. 2017

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

Leishmaniasis and trypanosomiasis are globally widespread parasitic diseases which have been responsible for high mortality rates. Since drugs available for their treatment are highly hepatotoxic, nephrotoxic and cardiotoxic, adherence to therapy has been affected. Thus, the search for new, more effective and safer drugs for the treatment of these diseases is necessary. Natural products have stood out as an alternative to searching for new bioactive molecules with therapeutic potential. In this study, the chemical composition and antiparasitic activity of the essential oil from Protium ovatum leaves against trypomastigote forms of Trypanosoma cruzi and the promastigote forms of Leishmania amazonensis were evaluated. The essential oil was promising against trypomastigote forms of T. cruzi (IC 50 = 28.55 μg.mL -1 ) and L.amazonensis promastigotes (IC 50 = 2.28 μg.mL -1 ). Eighteen chemical constituents were identified by GasChromatography coupled to Mass Spectrometry (GC-MS) in the essential oil, whose major constituents were spathulenol (17.6 %), caryophyllene oxide (16.4 %), β-caryophyllene (14.0 %) and myrcene (8.4 %).In addition, the essential oil from P. ovatum leaves had moderate cytotoxicity against LLCMK 2 adherent epithelial cell at the concentration range under analysis (CC 50 = 150.9 μg.mL -1 ). It should be highlighted that this is the first report of the chemical composition and anti-Trypanosoma cruzi and anti-Leishmania amazonensis activities of the essential oil from Protium ovatum leaves.

show abstract

Section: Resultsmentioning

confidence: 99%

In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae)

Estevam

Deus

Silva

et al. 2017

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

show abstract

“…In previous studies of the efficiency of the essential oil from leaves of P. ovatum, the result was 0.10 % and a complex mixture of terpene constituents was determined by 1 H NMR, 13 C NMR and IR (CASTELO et al 2010). Other species of Protium were analyzed and a complex mixture of monoterpenes and sesquiterpenes were determined in essential oils from resins, foliar rachises, branches and leaves (CARVALHO et al 2013;PINTO et al 2010;CARVALHO et al 2010). 139.0 (CH, C-9); 131.3 (C, C-3); 124.2 (CH, C-4); 115.5 (CH 2 , C-7); 112.7 (CH 2 , C-10); 31.4 (CH 2 , C-6); 26.9 (CH 2 , C-5); 25.7 (CH 3 , C-1) and 17.7 (CH 3 , C-2).…”

Section: Resultsmentioning

confidence: 99%

Chemical composition, anti-Trypanosoma cruzi and cytotoxic activities of the essential oil from green fruits of Protium ovatum (BURSERACEAE)

et al. 2018

View full text Add to dashboard Cite

-Chagas disease is a major neglected tropical disease caused by Trypanosoma cruzi. It has been treated with the antiparasitic drugs nifurtimox and benzonidazole, which cause several side effects. The market for natural products has considerably grown lately and the use of plants has become an alternative to the development of novel drugs to cure the disease. Therefore, this study aimed at describing the chemical analysis of the essential oil from green fruits of Protium ovatum and evaluating their trypanocidal and cytotoxic potential. The essential oil was obtained by Clevenger hydrodistillation whereas its chemical composition was determined by gas chromatography coupled to mass spectrometry (GC-MS). The major compounds found in the essential oil from green fruits of P. ovatum were β-myrcene (62.0 %), α-pinene (11.3 %) and limonene (7.3 %). To the best of our knowledge, this was the first time that the chemical composition of the essential oil from green fruits of P. ovatum was described. Results showed that the essential oil had strong trypanocidal activity against trypomastigote forms of theY strain of Trypanosoma cruzi (IC 50 = 1.2 µg/mL). In addition, the essential oil from green fruits of P. ovatum did not display cytotoxicity against LLCMK 2 adherent epithelial cell at the concentration range under analysis (CC 50 = 550.3 μg/mL). As a result, it is an excellent option for the development of novel antiparasitic drugs. Index terms: Protium ovatum, β-myrcene, fruits, essential oil, Trypanosoma cruzi, cytotoxic analysis. Composição química, atividades anti-Trypanosoma cruzi e citotóxica do óleo essencial dos frutos verdes de Protium ovatum (BURSERACEAE)Resumo -A doença de Chagas é uma das principais doenças tropicais negligenciadas causadas pelo Trypanosoma cruzi, e em seu tratamento utilizam-se medicamentos como o nifurtimox e o benzonidazol, que causam vários efeitos colaterais. O mercado de produtos naturais tem aumentado consideravelmente nos últimos anos, e o uso das plantas continua sendo uma alternativa para o desenvolvimento de novos medicamentos para cura de doenças. Portanto, este estudo aborda a composição química do óleo essencial dos frutos verdes de Protium ovatum e a avaliação de seus potenciais tripanocida e citotóxico. O óleo essencial foi obtido por hidrodestilação, utilizando o aparato do tipo Clevenger. A composição química foi determinada por cromatografia gasosa acoplada à espectrometria de massas (CG-EM). Os principais compostos encontrados no óleo essencial dos frutos verdes de P. ovatum foram: β-mirceno (62,0 %), α-pineno (11,3 %) e limoneno (7,3 %).

show abstract

“…The chemical composition of volatiles from Protium species from Ducke Reserve has been investigated and has shown that essential oils extracted by hydrodistillation are characterized by producing monoterpenes and sesquiterpenes. In most species, monoterpenes predominate in the essential oils of the resins (Pinto et al, 2010, Lima et al, 2016, Santana et al, 2020 and sesquiterpenes are predominant in aerial parts (Carvalho et al, 2009, Carvalho et al, 2013, Pinto et al, 2010, Oliveira et al, 2018, Lima et al, 2022. Now, three species from the Ducke Reserve have been selected for evaluation, namely, Protium divaricatum Engl.…”

Section: Introductionmentioning

confidence: 99%

Essential oils, chemical stimulation of resin and occurrence of galls in three Amazonian species of Protium

Lima¹,

Cunha²,

Melo³

et al. 2022

Acta Bras.

Self Cite

View full text Add to dashboard Cite

Protium is the most frequent genus of Burseraceae in the Adolpho Ducke Forest Reserve (Manaus, Amazonas), which is where its species were previously identified and mapped and this has favored various studies of this central Amazon forest. A number of P. divaricatum Engl., P. opacum Swart and P. strumosum Daly trees were selected with the objective of providing information about their chemical and biological aspects. Resin exudation was stimulated with the use of ethephon and the essential oils from the aerial parts and the resin were evaluated using GC. The occurrence of galls was also registered. The hydrocarbon sesquiterpene ß-caryophyllene was detected in high percentages in the essential oils of leaves and twigs of P. divaricatum (52.75% and 14.74%) and P. opacum (39.55% and 17.86%), respectively. The predominant oxygenated sesquiterpenes were spathulenol in the leaves (19.52%) and twigs (31.29%) of P. divaricatum, in addition to khusimone (32.36%) in the twigs of P. opacum. Monoterpenes were identified in the essential oils of the P. strumosum resin, with p-cymene (58.97%) predominating in the naturally exuded resin, limonene (61.45%) and p-cimen-8-ol (53.92%) in the resin of the first and second extraction with ethephon, respectively. On the trees, galls were identified that will help in the identification of the three species.

show abstract

Essential Oils ofProtiumof the Adolpho Ducke Forest Reserve:Protium crassipetalum, P. heptaphyllumsubs.ulei, P. pilosissimumandP. polybotryum

Cited by 6 publications

References 6 publications

In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae)

In vitro antiparasitic activity and chemical composition of the essential oil from Protium ovatum leaves (Burceraceae)

Chemical composition, anti-Trypanosoma cruzi and cytotoxic activities of the essential oil from green fruits of Protium ovatum (BURSERACEAE)

Essential oils, chemical stimulation of resin and occurrence of galls in three Amazonian species of Protium

Contact Info

Product

Resources

About