Essential oils of two Nepeta species inhibit growth and induce oxidative stress in ragweed (Ambrosia artemisiifolia L.) shoots in vitro

Cytotoxic activity of Nepeta rtanjensis Diklić & Milojević essential oil and its mode of action

“…Analysis of N. rtanjensis EO was performed by gas chromatog-raphy (GC) using two types of detectors, flame ionization (FID) and mass-spectrometric (MSD) as previously described (Dmitrović et al, 2015). GC-FID analysis of the EO was carried out on an AgilentTechnologies, model 5890A gas chromatograph on HP-5 column (25 m × 0.32 mm, 0.53 μm film thickness).…”

“…Antimicrobial activity againsta variety of bacteria and fungi, antioxidant and phytotoxic activi-ties of Nepeta plants have been well documented (Stojanović et al,2005;Tepe et al, 2007;Ljaljević Grbić et al, 2008;Nestorović et al,2010;Kumar et al, 2014). Furthermore, these plants have shown antiviral, phytotoxic and allelopathiceffects (Kobaisy et al, 2005;Formisano et al, 2011;Dmitrović et al,2015;Nestorović Zivković et al, 2016). Several studies investigatedthe cytostatic and cytotoxic activities of various Nepeta species Tsuruoka et al, 2012;Kahkeshani et al, 2014;Shakeri et al, 2014Shakeri et al, , 2016.…”

“…It is a rare and strictly endemicperennial found only in a few localities of Mt Rtanj in North-EastSerbia (Diklić, 1999;Mišić et al, 2005). Therefore, in vitro plantpropagation, field cultivation and reintroduction were performed (Mišić et al, 2005) in order to obtain sufficient plant material forthe investigation of biological activities (Ljaljević Grbić et al, 2008;Nestorović et al, 2010;Dmitrović et al, 2015;Nestorović Živković cet al, 2016). Until now various biological activities of N. rtanjen-sis are well demonstrated: antibacterial (Stojanović et al, 2005;Nestorović et al, 2010), antifungal (Stojanović et al, 2005;Ljaljević Grbić et al, 2008;Nestorović et al, 2010), allelopathic (Nestorović Živković et al, 2016), and phytotoxic (Dmitrović et al, 2015).…”

“…Therefore, in vitro plantpropagation, field cultivation and reintroduction were performed (Mišić et al, 2005) in order to obtain sufficient plant material forthe investigation of biological activities (Ljaljević Grbić et al, 2008;Nestorović et al, 2010;Dmitrović et al, 2015;Nestorović Živković cet al, 2016). Until now various biological activities of N. rtanjen-sis are well demonstrated: antibacterial (Stojanović et al, 2005;Nestorović et al, 2010), antifungal (Stojanović et al, 2005;Ljaljević Grbić et al, 2008;Nestorović et al, 2010), allelopathic (Nestorović Živković et al, 2016), and phytotoxic (Dmitrović et al, 2015). Theessential oil (EO) of N. rtanjensis is characterized by a high content oftrans-cis nepetalactone which is considered to be the main compo-nent principally responsible for the biological activities (Stojanovi´cet al, 2005;Nestorovi´c et al, 2010;Mišić et al, 2015).…”

Elicitation effects of synthetic 1,2,4,5-tetraoxane and 2,5-diphenyltiophene in shoot cultures of two Nepeta species

“…1 In folk medicine Nepeta species are used in the treatment of gastrointestinal disorders, cough and asthma due to their spasmolytic and myorelaxant activities. 2 Their anti-inflammatory, 3 cytostatic activity, 4 as well as phytotoxic effect on plants, [5][6][7][8] behavioral effect on cats, 9,10 and repellent activity against some insects 11 are well known. The plethora of published literature data explain antibacterial, antifungal and antiviral activity of many different species from genus Nepeta.…”